Restriction map for
pFN21AB7588
(Circular) (Six-base) MAPSORT of: pFN21AB7588.seq Check: 8501 from: 1 to: 5572
pFN21AB7588 5572 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2823 2823
Size: 5572
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2075 3382 278
Size: 53 83 186 936 539 1307 2468
Fragments arranged by size:
2468 1307 936 539 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 1937
Size: 978 4594
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 3635 5019 5287 1632
Size: 477 1526 1384 268 1917
Fragments arranged by size:
1917 1526 1384 477 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2759 2791 2555
Size: 32 172 32 5336
Fragments arranged by size:
5336 172 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3315 4887 3315
Size: 1572 4000
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2378 2897 1870
Size: 73 174 261 519 4545
Fragments arranged by size:
4545 519 261 174 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2773 2806 2773
Size: 33 5539
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2321 2893 4348 618
Size: 325 217 627 534 572 1455 1842
Fragments arranged by size:
1842 1455 627 572 534 325 217
BanII G_rGCy'C
Cuts at: 1391 2167 2379 2682 2690 2831 2891 1391
Size: 776 212 303 8 141 60 4072
Fragments arranged by size:
4072 776 303 212 141 60 8
BbeI G_GCGC'C
Cuts at: 2325 2325
Size: 5572
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2115 2483 928
Size: 971 216 368 4017
Fragments arranged by size:
4017 971 368 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4674 3464
Size: 1210 4362
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2513 4183 136
Size: 107 122 71 631 605 841 1670 1525
Fragments arranged by size:
1670 1525 841 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2702 2989 2702
Size: 287 5285
Bme1580I G_kGCm'C
Cuts at: 1790 3633 4789 1790
Size: 1843 1156 2573
Fragments arranged by size:
2573 1843 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2301 1056
Size: 1245 4327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2614 2649 2783 4232 1239
Size: 1375 35 134 1449 2579
Fragments arranged by size:
2579 1449 1375 134 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3697 4562 4860 3697
Size: 865 298 4409
Fragments arranged by size:
4409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4235 882
Size: 617 2736 2219
Fragments arranged by size:
2736 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3363 493
Size: 711 2159 2702
Fragments arranged by size:
2702 2159 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2322 3379 3761
Size: 53 83 186 936 539 250 1057 382
Cuts at: 3761 4396 275
Size: 635 1451
Fragments arranged by size:
1451 1057 936 635 539 382 250 186
83 53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 1178
Size: 30 165 30 882 30 4435
Fragments arranged by size:
4435 882 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5572
BseYI C'CCAG_C
Cuts at: 2033 2266 2524 2624 2746 4775 5271 2033
Size: 233 258 100 122 2029 496 2334
Fragments arranged by size:
2334 2029 496 258 233 122 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2811 1163
Size: 573 1075 3924
Fragments arranged by size:
3924 1075 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3022 3783 4811 1540
Size: 275 186 1021 761 1028 2301
Fragments arranged by size:
2301 1028 1021 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 3633 3718 4789 2619
Size: 1014 85 1071 3402
Fragments arranged by size:
3402 1071 1014 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
BsmBI CGTCTCn'nnnn_
Cuts at: 2100 2100
Size: 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2167 2379 2619 2682 2690 2831 2891
Size: 399 377 212 240 63 8 141 60
Cuts at: 2891 3633 3718 4789 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 377 240 212 141
85 63 60 8
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 2589 3461 5191 2589
Size: 872 1730 2970
Fragments arranged by size:
2970 1730 872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 2927 844
Size: 1979 104 3489
Fragments arranged by size:
3489 1979 104
BsrBI CCG'CTC
Cuts at: 1394 1823 2697 3459 1394
Size: 429 874 762 3507
Fragments arranged by size:
3507 874 762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4067 4241 65
Size: 1183 2819 174 1396
Fragments arranged by size:
2819 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4216 4403 1700
Size: 110 2406 187 2869
Fragments arranged by size:
2869 2406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5572
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstXI CCAn_nnnn'nTGG
Cuts at: 2673 2761 2673
Size: 88 5484
BstYI r'GATC_y
Cuts at: 1972 2570 2902 3269 3654 3671 5123 5209 5221
Size: 598 332 367 385 17 1452 86 12
Cuts at: 5221 5567 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 598 385 367 346 332 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5020
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3882 3902 3088
Size: 794 20 4758
Fragments arranged by size:
4758 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 2242 2242
Size: 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2027 4579 809
Size: 507 711 2552 1802
Fragments arranged by size:
2552 1802 711 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2451 2514 3019
Size: 54 1425 50 275 18 621 63 505
Cuts at: 3019 3908 5537 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 621 505 275 63 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 1537
Size: 275 1207 4090
Fragments arranged by size:
4090 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3502 1353
Size: 2149 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 4159 4533 4679 387
Size: 1064 138 653 1917 374 146 1280
Fragments arranged by size:
1917 1280 1064 653 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2614 2649 2783 3635 4232 5019
Size: 393 477 505 35 134 852 597 787
Cuts at: 5019 5287 1239
Size: 268 1524
Fragments arranged by size:
1524 852 787 597 505 477 393 268
134 35
EcoO109I rG'GnC_Cy
Cuts at: 2029 2029
Size: 5572
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 2325 2346 2852 4719 1678
Size: 647 21 506 1867 2531
Fragments arranged by size:
2531 1867 647 506 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2562 2594 4213 4245 4287
Size: 32 135 32 990 32 1619 32 42
Cuts at: 4287 4319 1373
Size: 32 2626
Fragments arranged by size:
2626 1619 990 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3162 669
Size: 988 281 978 246 3079
Fragments arranged by size:
3079 988 978 281 246
HindIII A'AGCT_T
Cuts at: 748 2274 748
Size: 1526 4046
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KasI G'GCGC_C
Cuts at: 2321 2321
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2753 2760 4686 4870 1305
Size: 1448 7 1926 184 2007
Fragments arranged by size:
2007 1926 1448 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3750
Fragments arranged by size:
3750 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2715 3530 3889 4048 518
Size: 648 1549 815 359 159 2042
Fragments arranged by size:
2042 1549 815 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2669 2845 2988 3665 4813 5058 1606
Size: 843 220 176 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 245 220 176 143
NarI GG'CG_CC
Cuts at: 2322 2322
Size: 5572
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5020
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 2297 1052
Size: 1245 4327
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2930 4475 1132
Size: 97 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2683 1348
Size: 1335 4237
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5572
PfoI T'CCnGG_A
Cuts at: 2858 2858
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2029 2029
Size: 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2924 830
Size: 2094 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
PvuII CAG'CTG
Cuts at: 2449 2449
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SanDI GG'GwC_CC
Cuts at: 2029 2029
Size: 5572
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SfcI C'TryA_G
Cuts at: 826 1045 2146 2287 2920 4055 4736 4927 826
Size: 219 1101 141 633 1135 681 191 1471
Fragments arranged by size:
1471 1135 1101 681 633 219 191 141
SfoI GGC'GCC
Cuts at: 2323 2323
Size: 5572
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3676 4577 4839 820
Size: 197 926 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 3011 513
Size: 552 1946 3074
Fragments arranged by size:
3074 1946 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2387 3599 3616 3769 3954 4399 325
Size: 1255 807 1212 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1212 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3818 5530 96
Size: 274 80 33 51 1443 1841 1712 138
Fragments arranged by size:
1841 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5532 5564 5532
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5572
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2638 2446
Size: 192 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2073 3380 276
Size: 53 83 186 936 539 1307 2468
Fragments arranged by size:
2468 1307 936 539 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI