Restriction map for
pFN21AB7587
(Circular) (Six-base) MAPSORT of: pFN21AB7587.seq Check: 2111 from: 1 to: 5377
pFN21AB7587 5377 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2720 1937
Size: 783 4594
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 2676 3504 3877 1563
Size: 1113 828 373 3063
Fragments arranged by size:
3063 1113 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 3440 4824 5092 1632
Size: 704 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1104 704 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 4276 5253 1203
Size: 3073 977 1327
Fragments arranged by size:
3073 1327 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5377
AlwNI CAG_nnn'CTG
Cuts at: 2200 2350 2407 3120 4692 2200
Size: 150 57 713 1572 2885
Fragments arranged by size:
2885 1572 713 150 57
ApaI G_GGCC'C
Cuts at: 2151 2466 2151
Size: 315 5062
ApaLI G'TGCA_C
Cuts at: 2529 3434 4590 2529
Size: 905 1156 3316
Fragments arranged by size:
3316 1156 905
ApoI r'AATT_y
Cuts at: 1493 2686 2912 3064 5173 1493
Size: 1193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2177 2702 1870
Size: 73 192 42 525 4545
Fragments arranged by size:
4545 525 192 73 42
BamHI G'GATC_C
Cuts at: 2707 3074 2707
Size: 367 5010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2476 2698 4153 618
Size: 325 217 627 689 222 1455 1842
Fragments arranged by size:
1842 1455 689 627 325 222 217
BanII G_rGCy'C
Cuts at: 1391 2124 2135 2151 2466 2696 1391
Size: 733 11 16 315 230 4072
Fragments arranged by size:
4072 733 315 230 16 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3568 3602 1903
Size: 34 1631 34 3678
Fragments arranged by size:
3678 1631 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 3988 136
Size: 107 122 71 631 605 637 1679 1525
Fragments arranged by size:
1679 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5372 5372
Size: 5377
BlpI GC'TnA_GC
Cuts at: 2411 2794 2411
Size: 383 4994
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2466 2481 2533 3438 4594 1790
Size: 361 315 15 52 905 1156 2573
Fragments arranged by size:
2573 1156 905 361 315 52 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2384 2555 4037 1239
Size: 830 315 171 1482 2579
Fragments arranged by size:
2579 1482 830 315 171
Bpu10I CC'TnA_GC
Cuts at: 2129 2129
Size: 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2270 3502 4367 4665 2174
Size: 96 1232 865 298 2886
Fragments arranged by size:
2886 1232 865 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3184 3566 4201 275
Size: 53 83 186 936 1651 382 635 1451
Fragments arranged by size:
1651 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3810 4208 4482 4629 5040 3810
Size: 398 274 147 411 4147
Fragments arranged by size:
4147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 2585 2615 1178
Size: 30 165 30 1086 30 66 30 3940
Fragments arranged by size:
3940 1086 165 66 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 4119
BseYI C'CCAG_C
Cuts at: 2167 2466 2480 4580 5076 2167
Size: 299 14 2100 496 2468
Fragments arranged by size:
2468 2100 496 299 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2582 1163
Size: 573 846 3958
Fragments arranged by size:
3958 846 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3588 4616 1540
Size: 275 186 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2184 2533 3438 3523 4594 2124
Size: 60 349 905 85 1071 2907
Fragments arranged by size:
2907 1071 905 349 85 60
BsmI GAATG_Cn'
Cuts at: 2492 2888 2981 2492
Size: 396 93 4888
Fragments arranged by size:
4888 396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2135 2151 2184 2466 2481 2533
Size: 399 334 11 16 33 282 15 52
Cuts at: 2533 2696 3438 3523 4594 1391
Size: 163 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 282 163 85
52 33 16 15 11
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2604 2732 844
Size: 1235 525 128 3489
Fragments arranged by size:
3489 1235 525 128
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 3264 1394
Size: 429 290 1151 3507
Fragments arranged by size:
3507 1151 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2247 4021 4208 1700
Size: 110 437 1774 187 2869
Fragments arranged by size:
2869 1774 437 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5377
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5377
BstBI TT'CG_AA
Cuts at: 2094 4181 2094
Size: 2087 3290
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5377
BstYI r'GATC_y
Cuts at: 1972 2364 2707 3074 3459 3476 4928 5014 5026
Size: 392 343 367 385 17 1452 86 12
Cuts at: 5026 5372 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 343 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2204 2241 513
Size: 552 939 200 37 3649
Fragments arranged by size:
3649 939 552 200 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2380 2533 2893 3687 3707 2380
Size: 153 360 794 20 4050
Fragments arranged by size:
4050 794 360 153 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2824 3713
Size: 54 1425 50 275 18 429 565 889
Cuts at: 3713 5342 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 565 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2824 1537
Size: 275 1012 4090
Fragments arranged by size:
4090 1012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 3307 1353
Size: 959 995 3423
Fragments arranged by size:
3423 995 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 4338 4484 387
Size: 1064 138 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2122
Size: 5377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2336 2384 2555 3440 4037 4824
Size: 393 437 267 48 171 885 597 787
Cuts at: 4824 5092 1239
Size: 268 1524
Fragments arranged by size:
1524 885 787 597 437 393 268 267
171 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 2423
Size: 5377
EcoO109I rG'GnC_Cy
Cuts at: 2140 2147 2148 2172 2404 2463 2140
Size: 7 1 24 232 59 5054
Fragments arranged by size:
5054 232 59 24 7 1
EcoRI G'AATT_C
Cuts at: 2686 3064 2686
Size: 378 4999
FspI TGC'GCA
Cuts at: 2372 3883 2372
Size: 1511 3866
HaeII r_GCGC'y
Cuts at: 1678 2398 2505 2547 4524 1678
Size: 720 107 42 1977 2531
Fragments arranged by size:
2531 1977 720 107 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4018 4050 4092 4124 1373
Size: 32 135 32 2446 32 42 32 2626
Fragments arranged by size:
2626 2446 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2721 2967 669
Size: 988 281 783 246 3079
Fragments arranged by size:
3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4491 4675 1305
Size: 3186 184 2007
Fragments arranged by size:
3186 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3555
Fragments arranged by size:
3555 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3335 3694 3853 518
Size: 648 1464 705 359 159 2042
Fragments arranged by size:
2042 1464 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2347 2793 3470 4618 4863 1606
Size: 741 446 677 1148 245 2120
Fragments arranged by size:
2120 1148 741 677 446 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3886
Fragments arranged by size:
3886 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2735 4280 1132
Size: 97 1506 1545 2229
Fragments arranged by size:
2229 1545 1506 97
PasI CC'CwG_GG
Cuts at: 1348 2144 2552 1348
Size: 796 408 4173
Fragments arranged by size:
4173 796 408
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5377
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2172 2172
Size: 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PspOMI G'GGCC_C
Cuts at: 2147 2462 2147
Size: 315 5062
PstI C_TGCA'G
Cuts at: 830 2513 2729 830
Size: 1683 216 3478
Fragments arranged by size:
3478 1683 216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
SacI G_AGCT'C
Cuts at: 2124 2124
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SanDI GG'GwC_CC
Cuts at: 2172 2172
Size: 5377
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2509 2580 2725 3860 4541 4732 826
Size: 219 1464 71 145 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 681 219 191 145 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5377
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2285 3481 4382 4644 820
Size: 197 926 210 132 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 262 210 197 132
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2735 1132
Size: 1603 3774
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 2303 1517
Size: 786 4591
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 2816 513
Size: 552 939 351 461 3074
Fragments arranged by size:
3074 939 552 461 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3421 3574 3759 4204 325
Size: 1255 1824 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 3623 5335
Size: 274 80 33 51 1443 408 1238 1712
Cuts at: 5335 96
Size: 138
Fragments arranged by size:
1712 1443 1238 408 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 5337 5369 2489
Size: 32 2816 32 2497
Fragments arranged by size:
2816 2497 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI