Restriction map for
pFN21AB7581
(Circular) (Six-base) MAPSORT of: pFN21AB7581.seq Check: 5604 from: 1 to: 5230
pFN21AB7581 5230 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 3293 4677 4945 1632
Size: 494 1167 1384 268 1917
Fragments arranged by size:
1917 1384 1167 494 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 2126 1166
Size: 960 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2040 2149 2973 4545 2040
Size: 109 824 1572 2725
Fragments arranged by size:
2725 1572 824 109
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BamHI G'GATC_C
Cuts at: 2501 2560 2927 2501
Size: 59 367 4804
Fragments arranged by size:
4804 367 59
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2486 2515 2549 1391
Size: 1095 29 34 4072
Fragments arranged by size:
4072 1095 34 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 928
Size: 971 162 4097
Fragments arranged by size:
4097 971 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 136
Size: 107 122 71 631 605 2169 1525
Fragments arranged by size:
2169 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 2961 3919
Size: 416 53 83 399 24 1064 480 958
Cuts at: 3919 442
Size: 1753
Fragments arranged by size:
1753 1064 958 480 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2529 3890 1239
Size: 1290 1361 2579
Fragments arranged by size:
2579 1361 1290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 3893 882
Size: 617 945 1449 2219
Fragments arranged by size:
2219 1449 945 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3037 3419 4054 275
Size: 53 83 186 936 1504 382 635 1451
Fragments arranged by size:
1504 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2473 2503 2514 2544 1178
Size: 30 165 30 1070 30 11 30 3864
Fragments arranged by size:
3864 1070 165 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2309 1186
Size: 919 204 4107
Fragments arranged by size:
4107 919 204
BseYI C'CCAG_C
Cuts at: 2382 2477 4433 4929 2382
Size: 95 1956 496 2683
Fragments arranged by size:
2683 1956 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2410 2428 1163
Size: 573 674 18 3965
Fragments arranged by size:
3965 674 573 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 3441 4469 1540
Size: 275 186 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 3291 3376 4447 3291
Size: 85 1071 4074
Fragments arranged by size:
4074 1071 85
BsmI GAATG_Cn'
Cuts at: 2326 2741 2834 2326
Size: 415 93 4722
Fragments arranged by size:
4722 415 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2486 2515 2549 3291 3376 4447 1391
Size: 399 696 29 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 399 85 34 29
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 844
Size: 1741 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 1394
Size: 429 1294 3507
Fragments arranged by size:
3507 1294 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 3874 4061 1700
Size: 110 349 1715 187 2869
Fragments arranged by size:
2869 1715 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5230
BstYI r'GATC_y
Cuts at: 1972 2501 2560 2927 3312 3329 4781 4867 4879
Size: 529 59 367 385 17 1452 86 12
Cuts at: 4879 5225 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 529 385 367 346 86 59
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 3619
Fragments arranged by size:
3619 939 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2231 487
Size: 683 56 1005 3486
Fragments arranged by size:
3486 1005 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2677 3566
Size: 54 1425 50 275 18 177 670 889
Cuts at: 3566 5195 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 670 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 1537
Size: 275 865 4090
Fragments arranged by size:
4090 865 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 3160 1353
Size: 1089 718 3423
Fragments arranged by size:
3423 1089 718
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3817 4191 4337 387
Size: 1064 138 2228 374 146 1280
Fragments arranged by size:
2228 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2529 3293 3890 4677 4945 1239
Size: 393 494 403 764 597 787 268 1524
Fragments arranged by size:
1524 787 764 597 494 403 393 268
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 2025 2462 4377 1678
Size: 347 437 1915 2531
Fragments arranged by size:
2531 1915 437 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2473 2505 3871
Size: 32 135 32 479 32 390 32 1366
Cuts at: 3871 3903 3945 3977 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1366 479 390 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2574 2820 669
Size: 988 281 636 246 3079
Fragments arranged by size:
3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4528 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 3188 3547 3706 518
Size: 648 960 1062 359 159 2042
Fragments arranged by size:
2042 1062 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2386 2646 3323 4471 4716 1606
Size: 431 349 260 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 431 349 260 245
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 5230
NcoI C'CATG_G
Cuts at: 513 1065 2004 2124 513
Size: 552 939 120 3619
Fragments arranged by size:
3619 939 552 120
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PfoI T'CCnGG_A
Cuts at: 2268 2268
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2578 3713 4394 4585 826
Size: 219 1533 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2325 2669 513
Size: 552 939 120 201 344 3074
Fragments arranged by size:
3074 939 552 344 201 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3274 3427 3612 4057 325
Size: 1255 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3476 5188 96
Size: 274 80 33 51 1443 1499 1712 138
Fragments arranged by size:
1712 1499 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI