Restriction map for
pFN21AB7578
(Circular) (Six-base) MAPSORT of: pFN21AB7578.seq Check: 2592 from: 1 to: 5557
pFN21AB7578 5557 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2814 3620 5004 5272 1632
Size: 1182 806 1384 268 1917
Fragments arranged by size:
1917 1384 1182 806 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 2715 4456 5433 1203
Size: 1512 1741 977 1327
Fragments arranged by size:
1741 1512 1327 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2077 2309 2512 2797 3300 4872 2077
Size: 232 203 285 503 1572 2762
Fragments arranged by size:
2762 1572 503 285 232 203
ApaI G_GGCC'C
Cuts at: 2234 2316 2234
Size: 82 5475
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 2176 2317 2882 1870
Size: 73 212 21 141 565 4545
Fragments arranged by size:
4545 565 212 141 73 21
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2296 2330 2643 2676 2878
Size: 325 217 627 509 34 313 33 202
Cuts at: 2878 4333 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 509 325 313 217 202
34 33
BanII G_rGCy'C
Cuts at: 1391 2234 2316 2844 2876 1391
Size: 843 82 528 32 4072
Fragments arranged by size:
4072 843 528 82 32
BbeI G_GCGC'C
Cuts at: 2300 2334 2647 2300
Size: 34 313 5210
Fragments arranged by size:
5210 313 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2156 3449 4659 2156
Size: 1293 1210 3054
Fragments arranged by size:
3054 1293 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 2439 2734
Size: 107 122 71 631 605 566 201 295
Cuts at: 2734 4168 136
Size: 1434 1525
Fragments arranged by size:
1525 1434 631 605 566 295 201 122
107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2234 2316 3618 4774 1790
Size: 444 82 1302 1156 2573
Fragments arranged by size:
2573 1302 1156 444 82
BmgBI CAC'GTC
Cuts at: 1318 2490 1318
Size: 1172 4385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 4246 442
Size: 416 53 83 399 24 1871 958 1753
Fragments arranged by size:
1871 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 4217 1239
Size: 1295 1683 2579
Fragments arranged by size:
2579 1683 1295
Bpu10I CC'TnA_GC
Cuts at: 2078 2078
Size: 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3348 493
Size: 711 2144 2702
Fragments arranged by size:
2702 2144 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2297 2331 2644 3364
Size: 53 83 186 936 764 34 313 720
Cuts at: 3364 3746 4381 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 764 720 635 382 313 186
83 53 34
BsaWI w'CCGG_w
Cuts at: 2222 2457 3990 4388 4662 4809 5220 2222
Size: 235 1533 398 274 147 411 2559
Fragments arranged by size:
2559 1533 411 398 274 235 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 1178
Size: 30 165 30 616 30 4686
Fragments arranged by size:
4686 616 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2672 1186
Size: 1486 4071
BseYI C'CCAG_C
Cuts at: 2020 2288 4760 5256 2020
Size: 268 2472 496 2321
Fragments arranged by size:
2472 2321 496 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2750 1163
Size: 573 1014 3970
Fragments arranged by size:
3970 1014 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2377 2729 2738 3007 3768 4796
Size: 275 186 376 352 9 269 761 1028
Cuts at: 4796 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 376 352 275 269 186
9
BsiHKAI G_wGCw'C
Cuts at: 3618 3703 4774 3618
Size: 85 1071 4401
Fragments arranged by size:
4401 1071 85
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2316 2844 2876 3618 3703 4774
Size: 399 444 82 528 32 742 85 1071
Cuts at: 4774 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 528 444 399 85 82
32
BspEI T'CCGG_A
Cuts at: 2222 5220 2222
Size: 2998 2559
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2805 2912 844
Size: 1961 107 3489
Fragments arranged by size:
3489 1961 107
BsrBI CCG'CTC
Cuts at: 1394 1823 2082 2502 2524 2674 3444 1394
Size: 429 259 420 22 150 770 3507
Fragments arranged by size:
3507 770 429 420 259 150 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 2394 2688 4201 4388 1700
Size: 110 382 202 294 1513 187 2869
Fragments arranged by size:
2869 1513 382 294 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 2045 3617 4629 2045
Size: 1572 1012 2973
Fragments arranged by size:
2973 1572 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2797 794
Size: 2003 3554
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstXI CCAn_nnnn'nTGG
Cuts at: 2135 2135
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2073 2887 3254 3639 3656 5108 5194 5206
Size: 101 814 367 385 17 1452 86 12
Cuts at: 5206 5552 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 814 385 367 346 101 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2167 2723 513
Size: 552 939 163 556 3347
Fragments arranged by size:
3347 939 556 552 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 3073 3867 3887 2310
Size: 763 794 20 3980
Fragments arranged by size:
3980 794 763 20
ClaI AT'CG_AT
Cuts at: 1554 2067 1554
Size: 513 5044
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DraIII CAC_nnn'GTG
Cuts at: 2637 2637
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2603 4564 809
Size: 507 1287 1961 1802
Fragments arranged by size:
1961 1802 1287 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2374 2574 2726
Size: 54 1425 50 275 18 544 200 152
Cuts at: 2726 2735 3004 3893 5522 8
Size: 9 269 889 1629 43
Fragments arranged by size:
1629 1425 889 544 275 269 200 152
54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2374 2726 2735 3004 1537
Size: 275 562 352 9 269 4090
Fragments arranged by size:
4090 562 352 275 269 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2786 3487 1353
Size: 1433 701 3423
Fragments arranged by size:
3423 1433 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2669 4144 4518 4664 387
Size: 1064 138 1080 1475 374 146 1280
Fragments arranged by size:
1475 1280 1080 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 2814 3620 4217 5004 5272 1239
Size: 393 902 280 806 597 787 268 1524
Fragments arranged by size:
1524 902 806 787 597 393 280 268
EcoO109I rG'GnC_Cy
Cuts at: 2313 2313
Size: 5557
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FspI TGC'GCA
Cuts at: 2259 2588 4063 2259
Size: 329 1475 3753
Fragments arranged by size:
3753 1475 329
HaeII r_GCGC'y
Cuts at: 1678 2300 2334 2530 2573 2647 4704 1678
Size: 622 34 196 43 74 2057 2531
Fragments arranged by size:
2531 2057 622 196 74 43 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 4198 4230 4272
Size: 32 135 32 436 32 2158 32 42
Cuts at: 4272 4304 1373
Size: 32 2626
Fragments arranged by size:
2626 2158 436 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KasI G'GCGC_C
Cuts at: 2296 2330 2643 2296
Size: 34 313 5210
Fragments arranged by size:
5210 313 34
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 2715 5433 2715
Size: 2718 2839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2499 4671 4855 1305
Size: 1194 2172 184 2007
Fragments arranged by size:
2172 2007 1194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 10
Size: 54 1425 343 744 2991
Fragments arranged by size:
2991 1425 744 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3515 3874 4033 518
Size: 648 2349 359 159 2042
Fragments arranged by size:
2349 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 2269 2288 2373 2731 2743 2973 3650
Size: 603 60 19 85 358 12 230 677
Cuts at: 3650 4798 5043 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 603 358 245 230 85
60 19 12
NaeI GCC'GGC
Cuts at: 2194 2690 2194
Size: 496 5061
NarI GG'CG_CC
Cuts at: 2297 2331 2644 2297
Size: 34 313 5210
Fragments arranged by size:
5210 313 34
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NgoMIV G'CCGG_C
Cuts at: 2192 2688 2192
Size: 496 5061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 2374 2735 3004 2374
Size: 361 269 4927
Fragments arranged by size:
4927 361 269
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2096 2805 1348
Size: 748 709 4100
Fragments arranged by size:
4100 748 709
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PspOMI G'GGCC_C
Cuts at: 2230 2312 2230
Size: 82 5475
PstI C_TGCA'G
Cuts at: 830 2305 2709 2793 2802 2909 830
Size: 1475 404 84 9 107 3478
Fragments arranged by size:
3478 1475 404 107 84 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SapI GCTCTTCn'nnn_
Cuts at: 2786 2786
Size: 5557
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2301 2705 2789 2798 2905 4040 4721
Size: 219 1256 404 84 9 107 1135 681
Cuts at: 4721 4912 826
Size: 191 1471
Fragments arranged by size:
1471 1256 1135 681 404 219 191 107
84 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2734 2734
Size: 5557
SfoI GGC'GCC
Cuts at: 2298 2332 2645 2298
Size: 34 313 5210
Fragments arranged by size:
5210 313 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3661 4562 4824 820
Size: 197 926 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 2112 2340 2582 1517
Size: 595 228 242 4492
Fragments arranged by size:
4492 595 242 228
StyI C'CwwG_G
Cuts at: 513 1065 2004 2388 2577 2996 513
Size: 552 939 384 189 419 3074
Fragments arranged by size:
3074 939 552 419 384 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2609 3584 3601 3754 3939 4384 325
Size: 1255 1029 975 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1029 975 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 5517 5549 2019
Size: 32 3466 32 2027
Fragments arranged by size:
3466 2027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SrfI SwaI XcmI