Restriction map for
pFN21AB7577
(Circular) (Six-base) MAPSORT of: pFN21AB7577.seq Check: 2544 from: 1 to: 5359
pFN21AB7577 5359 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3422 4806 5074 1632
Size: 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AlwNI CAG_nnn'CTG
Cuts at: 2177 2408 3102 4674 2177
Size: 231 694 1572 2862
Fragments arranged by size:
2862 1572 694 231
ApaI G_GGCC'C
Cuts at: 2072 2072
Size: 5359
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2486 2668 2894 3046 5155 1493
Size: 993 182 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2065 2684 1870
Size: 73 90 32 619 4545
Fragments arranged by size:
4545 619 90 73 32
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2260 2680 4135 618
Size: 325 217 627 254 219 420 1455 1842
Fragments arranged by size:
1842 1455 627 420 325 254 219 217
BanII G_rGCy'C
Cuts at: 1391 2072 2678 1391
Size: 681 606 4072
Fragments arranged by size:
4072 681 606
BbeI G_GCGC'C
Cuts at: 2045 2264 2045
Size: 219 5140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4388
BbvCI CC'TCA_GC
Cuts at: 2615 2615
Size: 5359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 3970 136
Size: 107 122 71 631 605 388 1910 1525
Fragments arranged by size:
1910 1525 631 605 388 122 107 71
BglII A'GATC_T
Cuts at: 5354 5354
Size: 5359
BlpI GC'TnA_GC
Cuts at: 2220 2776 2220
Size: 556 4803
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2186 2540 3420 4576 1790
Size: 282 114 354 880 1156 2573
Fragments arranged by size:
2573 1156 880 354 282 114
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 4354
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3090 4048 442
Size: 416 53 83 399 24 1673 958 1753
Fragments arranged by size:
1753 1673 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2092 2187 4019 1239
Size: 853 95 1832 2579
Fragments arranged by size:
2579 1832 853 95
Bpu10I CC'TnA_GC
Cuts at: 2473 2615 2473
Size: 142 5217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3484 4349 4647 3484
Size: 865 298 4196
Fragments arranged by size:
4196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2089 4022 882
Size: 617 590 1933 2219
Fragments arranged by size:
2219 1933 617 590
BsaAI yAC'GTr
Cuts at: 493 1204 2296 3150 493
Size: 711 1092 854 2702
Fragments arranged by size:
2702 1092 854 711
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2261 3166 3548
Size: 53 83 186 936 509 219 905 382
Cuts at: 3548 4183 275
Size: 635 1451
Fragments arranged by size:
1451 936 905 635 509 382 219 186
83 53
BsaWI w'CCGG_w
Cuts at: 2239 3792 4190 4464 4611 5022 2239
Size: 1553 398 274 147 411 2576
Fragments arranged by size:
2576 1553 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5134
Fragments arranged by size:
5134 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5359
BseYI C'CCAG_C
Cuts at: 2177 4562 5058 2177
Size: 2385 496 2478
Fragments arranged by size:
2478 2385 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4786
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3420 3505 4576 3420
Size: 85 1071 4203
Fragments arranged by size:
4203 1071 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
BsmBI CGTCTCn'nnnn_
Cuts at: 2313 2313
Size: 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2186 2540 2678 3420 3505 4576
Size: 399 282 114 354 138 742 85 1071
Cuts at: 4576 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 354 282 138 114
85
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2714 844
Size: 1870 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 3246 1394
Size: 429 401 1022 3507
Fragments arranged by size:
3507 1022 429 401
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4003 4190 1700
Size: 110 234 1959 187 2869
Fragments arranged by size:
2869 1959 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5359
BssSI C'ACGA_G
Cuts at: 3419 4431 3419
Size: 1012 4347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5359
BstYI r'GATC_y
Cuts at: 1972 2689 3056 3441 3458 4910 4996 5008 5354
Size: 717 367 385 17 1452 86 12 346
Cuts at: 5354 1972
Size: 1977
Fragments arranged by size:
1977 1452 717 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2118 2139 2331 2592 513
Size: 552 1053 21 192 261 3280
Fragments arranged by size:
3280 1053 552 261 192 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 2521 2875 3669 3689 2409
Size: 112 354 794 20 4079
Fragments arranged by size:
4079 794 354 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2806 3695
Size: 54 1425 50 275 18 480 496 889
Cuts at: 3695 5324 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 496 480 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3289 1353
Size: 1936 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 3946 4320 4466 387
Size: 1064 138 452 1905 374 146 1280
Fragments arranged by size:
1905 1280 1064 452 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2187 3422 4019 4806 5074 1239
Size: 393 460 95 1235 597 787 268 1524
Fragments arranged by size:
1524 1235 787 597 460 393 268 95
EcoO109I rG'GnC_Cy
Cuts at: 2069 2156 2189 2069
Size: 87 33 5239
Fragments arranged by size:
5239 87 33
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2045 2264 4506 1678
Size: 367 219 2242 2531
Fragments arranged by size:
2531 2242 367 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373
Size: 32 135 32 2428 32 42 32 2626
Fragments arranged by size:
2626 2428 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KasI G'GCGC_C
Cuts at: 2041 2260 2041
Size: 219 5140
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4473 4657 1305
Size: 3168 184 2007
Fragments arranged by size:
3168 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3537
Fragments arranged by size:
3537 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 3317 3676 3835 518
Size: 648 1467 684 359 159 2042
Fragments arranged by size:
2042 1467 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2120 2141 2150 2174 2219 2371 2775
Size: 410 104 21 9 24 45 152 404
Cuts at: 2775 3452 4600 4845 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 410 404 245 152 104
45 24 21 9
NaeI GCC'GGC
Cuts at: 2046 2046
Size: 5359
NarI GG'CG_CC
Cuts at: 2042 2261 2042
Size: 219 5140
NcoI C'CATG_G
Cuts at: 513 1065 2592 513
Size: 552 1527 3280
Fragments arranged by size:
3280 1527 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NgoMIV G'CCGG_C
Cuts at: 2044 2044
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2717 4262 1132
Size: 97 1488 1545 2229
Fragments arranged by size:
2229 1545 1488 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5359
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5359
PfoI T'CCnGG_A
Cuts at: 2555 2555
Size: 5359
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2156 2189 2156
Size: 33 5326
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PspOMI G'GGCC_C
Cuts at: 2068 2068
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2015 2711 830
Size: 1185 696 3478
Fragments arranged by size:
3478 1185 696
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
PvuII CAG'CTG
Cuts at: 2174 2219 2371 2174
Size: 45 152 5162
Fragments arranged by size:
5162 152 45
SacII CC_GC'GG
Cuts at: 2121 2142 2121
Size: 21 5338
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SanDI GG'GwC_CC
Cuts at: 2189 2189
Size: 5359
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2011 2516 2707 3842 4523 4714 826
Size: 219 966 505 191 1135 681 191 1471
Fragments arranged by size:
1471 1135 966 681 505 219 191 191
SfoI GGC'GCC
Cuts at: 2043 2262 2043
Size: 219 5140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2067 2686 1872
Size: 195 619 4545
Fragments arranged by size:
4545 619 195
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 4364 4626 820
Size: 197 926 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2296 3150 493
Size: 1803 854 2702
Fragments arranged by size:
2702 1803 854
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 2441 1517
Size: 924 4435
StyI C'CwwG_G
Cuts at: 513 1065 2193 2592 2798 513
Size: 552 1128 399 206 3074
Fragments arranged by size:
3074 1128 552 399 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3605 5317
Size: 274 80 33 51 1443 603 1025 1712
Cuts at: 5317 96
Size: 138
Fragments arranged by size:
1712 1443 1025 603 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2347
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2065 2684 1870
Size: 195 619 4545
Fragments arranged by size:
4545 619 195
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI