Restriction map for
pFN21AB7574
(Circular) (Six-base) MAPSORT of: pFN21AB7574.seq Check: 6455 from: 1 to: 5548
pFN21AB7574 5548 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 2847 3675 4048 1563
Size: 1284 828 373 3063
Fragments arranged by size:
3063 1284 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2769 3611 4995 5263 1632
Size: 1137 842 1384 268 1917
Fragments arranged by size:
1917 1384 1137 842 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 2506 3291 4863 2506
Size: 785 1572 3191
Fragments arranged by size:
3191 1572 785
ApaLI G'TGCA_C
Cuts at: 2669 3605 4761 2669
Size: 936 1156 3456
Fragments arranged by size:
3456 1156 936
ApoI r'AATT_y
Cuts at: 1493 2225 2857 3083 3235 5344 1493
Size: 732 632 226 152 2109 1697
Fragments arranged by size:
2109 1697 732 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2130 4102 160
Size: 872 1098 1972 1606
Fragments arranged by size:
1972 1606 1098 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2252 2873 1870
Size: 73 309 621 4545
Fragments arranged by size:
4545 621 309 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 2869 4324 618
Size: 325 217 627 292 790 1455 1842
Fragments arranged by size:
1842 1455 790 627 325 292 217
BanII G_rGCy'C
Cuts at: 1391 2867 1391
Size: 1476 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2661 928
Size: 971 762 3815
Fragments arranged by size:
3815 971 762
BbvCI CC'TCA_GC
Cuts at: 2814 2814
Size: 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4159 136
Size: 107 122 71 631 605 2487 1525
Fragments arranged by size:
2487 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2243 5543 2243
Size: 3300 2248
BlpI GC'TnA_GC
Cuts at: 2499 2965 2499
Size: 466 5082
Bme1580I G_kGCm'C
Cuts at: 1790 2673 3609 4765 1790
Size: 883 936 1156 2573
Fragments arranged by size:
2573 1156 936 883
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2031 2300 3279
Size: 416 53 83 399 24 614 269 979
Cuts at: 3279 4237 442
Size: 958 1753
Fragments arranged by size:
1753 979 958 614 416 399 269 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 2187
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 4208 1239
Size: 1362 1607 2579
Fragments arranged by size:
2579 1607 1362
Bpu10I CC'TnA_GC
Cuts at: 2814 2814
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3673 4538 4836 3673
Size: 865 298 4385
Fragments arranged by size:
4385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2079 2179 4211 882
Size: 617 580 100 2032 2219
Fragments arranged by size:
2219 2032 617 580 100
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3355 3737 4372 275
Size: 53 83 186 936 1822 382 635 1451
Fragments arranged by size:
1822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2025 2055 1178
Size: 30 165 30 622 30 4671
Fragments arranged by size:
4671 622 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2075 1186
Size: 847 42 4659
Fragments arranged by size:
4659 847 42
BseYI C'CCAG_C
Cuts at: 4751 5247 4751
Size: 496 5052
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4975
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2021 2998 3759 4787 1540
Size: 275 186 20 977 761 1028 2301
Fragments arranged by size:
2301 1028 977 761 275 186 20
BsiHKAI G_wGCw'C
Cuts at: 2500 2673 2684 2780 3609 3694 4765 2500
Size: 173 11 96 829 85 1071 3283
Fragments arranged by size:
3283 1071 829 173 96 85 11
BsmI GAATG_Cn'
Cuts at: 2574 3059 3152 2574
Size: 485 93 4970
Fragments arranged by size:
4970 485 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2057 2057
Size: 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2500 2673 2684 2780 2867 3609 3694
Size: 399 710 173 11 96 87 742 85
Cuts at: 3694 4765 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 710 399 173 96 87
85 11
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2466 2601 4043 4217 65
Size: 1183 1218 135 1442 174 1396
Fragments arranged by size:
1442 1396 1218 1183 174 135
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 4192 4379 1700
Size: 110 210 2172 187 2869
Fragments arranged by size:
2869 2172 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 2146 96
Size: 2050 3498
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2098 2098
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2233 2243 2387 2878 3245 3630 3647 5099
Size: 261 10 144 491 367 385 17 1452
Cuts at: 5099 5185 5197 5543 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 491 385 367 346 261 144
86 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2796 2796
Size: 5548
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4996
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3858 3878 3064
Size: 794 20 4734
Fragments arranged by size:
4734 794 20
ClaI AT'CG_AT
Cuts at: 1554 2697 1554
Size: 1143 4405
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2995 3884
Size: 54 1425 50 275 18 188 977 889
Cuts at: 3884 5513 8
Size: 1629 43
Fragments arranged by size:
1629 1425 977 889 275 188 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2018 2995 1537
Size: 275 206 977 4090
Fragments arranged by size:
4090 977 275 206
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2770 3478 1353
Size: 824 593 708 3423
Fragments arranged by size:
3423 824 708 593
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2601 2769 3611 4208 4995 5263 1239
Size: 393 969 168 842 597 787 268 1524
Fragments arranged by size:
1524 969 842 787 597 393 268 168
EcoO109I rG'GnC_Cy
Cuts at: 2104 2760 2792 2104
Size: 656 32 4860
Fragments arranged by size:
4860 656 32
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2849 2849
Size: 5548
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 4695 1678
Size: 3017 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2767 2799 4189
Size: 32 135 32 504 32 659 32 1390
Cuts at: 4189 4221 4263 4295 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1390 659 504 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2517 2892 3138 669
Size: 988 281 579 375 246 3079
Fragments arranged by size:
3079 988 579 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 2517 3138 2517
Size: 621 4927
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 3506 3865 4024 518
Size: 648 930 1410 359 159 2042
Fragments arranged by size:
2042 1410 930 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2506 2964 3641 4789 5034 1606
Size: 411 489 458 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 458 411 245
NaeI GCC'GGC
Cuts at: 2022 2022
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 2548 387
Size: 2161 3387
NgoMIV G'CCGG_C
Cuts at: 2020 2020
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PacI TTA_AT'TAA
Cuts at: 2487 2487
Size: 5548
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2760 2760
Size: 5548
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2900 830
Size: 2070 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2506 2506
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2432 2896 4031 4712 4903 826
Size: 219 1387 464 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 681 464 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2254 2875 1872
Size: 382 621 4545
Fragments arranged by size:
4545 621 382
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3652 4553 4815 820
Size: 197 926 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 2582 1517
Size: 1065 4483
StyI C'CwwG_G
Cuts at: 513 1065 2987 513
Size: 552 1922 3074
Fragments arranged by size:
3074 1922 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2146 3794 5506
Size: 274 80 33 51 1443 169 1648 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1712 1648 1443 274 169 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2044
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2252 2873 1870
Size: 382 621 4545
Fragments arranged by size:
4545 621 382
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI