Restriction map for
pFN21AB7573
(Circular) (Six-base) MAPSORT of: pFN21AB7573.seq Check: 8906 from: 1 to: 5341
pFN21AB7573 5341 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2608 2608
Size: 5341
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3151 278
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 2684 1937
Size: 616 131 4594
Fragments arranged by size:
4594 616 131
Acc65I G'GTAC_C
Cuts at: 2352 2662 2352
Size: 310 5031
AclI AA'CG_TT
Cuts at: 1563 3468 3841 1563
Size: 1905 373 3063
Fragments arranged by size:
3063 1905 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3404 4788 5056 1632
Size: 1772 1384 268 1917
Fragments arranged by size:
1917 1772 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5144
AflIII A'CryG_T
Cuts at: 1203 4240 5217 1203
Size: 3037 977 1327
Fragments arranged by size:
3037 1327 977
AgeI A'CCGG_T
Cuts at: 4172 4172
Size: 5341
AhdI GACnn_n'nnGTC
Cuts at: 4070 4070
Size: 5341
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5341
AlwNI CAG_nnn'CTG
Cuts at: 3084 4656 3084
Size: 1572 3769
ApaI G_GGCC'C
Cuts at: 2403 2403
Size: 5341
ApaLI G'TGCA_C
Cuts at: 3398 4554 3398
Size: 1156 4185
ApoI r'AATT_y
Cuts at: 1493 2650 2876 3028 5137 1493
Size: 1157 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3895 160
Size: 872 2863 1606
Fragments arranged by size:
2863 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
AvaI C'yCGr_G
Cuts at: 1870 1943 2340 2409 2477 2666 1870
Size: 73 397 69 68 189 4545
Fragments arranged by size:
4545 397 189 73 69 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2559 2592 2559
Size: 33 5308
BamHI G'GATC_C
Cuts at: 2671 3038 2671
Size: 367 4974
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 2352 2620 2662 4117
Size: 325 217 627 558 7 268 42 1455
Cuts at: 4117 618
Size: 1842
Fragments arranged by size:
1842 1455 627 558 325 268 217 42
7
BanII G_rGCy'C
Cuts at: 1391 2073 2403 2521 2660 1391
Size: 682 330 118 139 4072
Fragments arranged by size:
4072 682 330 139 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3532 3566 1903
Size: 34 1595 34 3678
Fragments arranged by size:
3678 1595 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3233 4443 3233
Size: 1210 4131
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3952 136
Size: 107 122 71 631 605 2280 1525
Fragments arranged by size:
2280 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5336 5336
Size: 5341
BlpI GC'TnA_GC
Cuts at: 2758 2758
Size: 5341
Bme1580I G_kGCm'C
Cuts at: 1790 2348 2387 2403 2410 3402 4558 1790
Size: 558 39 16 7 992 1156 2573
Fragments arranged by size:
2573 1156 992 558 39 16 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2397 3072 4030
Size: 416 53 83 399 24 980 675 958
Cuts at: 4030 442
Size: 1753
Fragments arranged by size:
1753 980 958 675 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2485 4001 1239
Size: 1246 1516 2579
Fragments arranged by size:
2579 1516 1246
Bpu10I CC'TnA_GC
Cuts at: 2319 2319
Size: 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 3466 4331 4629 2282
Size: 1184 865 298 2994
Fragments arranged by size:
2994 1184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 4004 882
Size: 617 831 1674 2219
Fragments arranged by size:
2219 1674 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 3132 493
Size: 711 1928 2702
Fragments arranged by size:
2702 1928 711
BsaBI GATnn'nnATC
Cuts at: 5335 5335
Size: 5341
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 3530 4165 275
Size: 53 83 186 936 1615 382 635 1451
Fragments arranged by size:
1615 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3774 4172 4446 4593 5004 3774
Size: 398 274 147 411 4111
Fragments arranged by size:
4111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 1178
Size: 30 165 30 1014 30 4072
Fragments arranged by size:
4072 1014 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5341
BseYI C'CCAG_C
Cuts at: 2560 2630 4544 5040 2560
Size: 70 1914 496 2861
Fragments arranged by size:
2861 1914 496 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4768
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 2791 3552 4580 1540
Size: 275 186 150 640 761 1028 2301
Fragments arranged by size:
2301 1028 761 640 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 2073 3402 3487 4558 2073
Size: 1329 85 1071 2856
Fragments arranged by size:
2856 1329 1071 85
BsmI GAATG_Cn'
Cuts at: 2852 2945 2852
Size: 93 5248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2348 2387 2403 2410 2521 2660
Size: 399 283 275 39 16 7 111 139
Cuts at: 2660 3402 3487 4558 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 275 139 111
85 39 16 7
BspEI T'CCGG_A
Cuts at: 5004 5004
Size: 5341
BspHI T'CATG_A
Cuts at: 3230 4960 3230
Size: 1730 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2608 2696 844
Size: 1764 88 3489
Fragments arranged by size:
3489 1764 88
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 1394
Size: 429 1405 3507
Fragments arranged by size:
3507 1405 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3836 4010 65
Size: 1183 2588 174 1396
Fragments arranged by size:
2588 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2623 3985 4172 1700
Size: 110 813 1362 187 2869
Fragments arranged by size:
2869 1362 813 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5341
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5341
BssSI C'ACGA_G
Cuts at: 3401 4413 3401
Size: 1012 4329
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2620 794
Size: 1826 3515
BstBI TT'CG_AA
Cuts at: 4145 4145
Size: 5341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5341
BstYI r'GATC_y
Cuts at: 1972 2601 2671 3038 3423 3440 4892 4978 4990
Size: 629 70 367 385 17 1452 86 12
Cuts at: 4990 5336 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 629 385 367 346 86 70
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2571 513
Size: 552 939 567 3283
Fragments arranged by size:
3283 939 567 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4602
Fragments arranged by size:
4602 683 56
BtsI GCAGTG_nn'
Cuts at: 2532 2857 3651 3671 2532
Size: 325 794 20 4202
Fragments arranged by size:
4202 794 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5341
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5306
DraI TTT'AAA
Cuts at: 2645 2645
Size: 5341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 4348 809
Size: 507 704 2328 1802
Fragments arranged by size:
2328 1802 704 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2148 2788
Size: 54 1425 50 275 18 207 111 640
Cuts at: 2788 3677 5306 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 640 275 207 111 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 2788 1537
Size: 275 336 640 4090
Fragments arranged by size:
4090 640 336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2597 3271 1353
Size: 1244 674 3423
Fragments arranged by size:
3423 1244 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3928 4302 4448 387
Size: 1064 138 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 5341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2485 3404 4001 4788 5056 1239
Size: 393 853 919 597 787 268 1524
Fragments arranged by size:
1524 919 853 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 2375
Size: 5341
EcoO109I rG'GnC_Cy
Cuts at: 2399 2399
Size: 5341
EcoRI G'AATT_C
Cuts at: 2650 3028 2650
Size: 378 4963
FspI TGC'GCA
Cuts at: 3847 3847
Size: 5341
HaeII r_GCGC'y
Cuts at: 1678 4488 1678
Size: 2810 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373
Size: 32 135 32 2410 32 42 32 2626
Fragments arranged by size:
2626 2410 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2685 2931 669
Size: 988 281 747 246 3079
Fragments arranged by size:
3079 988 747 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5341
HpaI GTT'AAC
Cuts at: 2931 2931
Size: 5341
KpnI G_GTAC'C
Cuts at: 2356 2666 2356
Size: 310 5031
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 5341
MluI A'CGCG_T
Cuts at: 5217 5217
Size: 5341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4455 4639 1305
Size: 3150 184 2007
Fragments arranged by size:
3150 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3519
Fragments arranged by size:
3519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2420 3299 3658 3817 518
Size: 648 843 411 879 359 159 2042
Fragments arranged by size:
2042 879 843 648 411 359 159
MspA1I CmG'CkG
Cuts at: 1606 2757 3434 4582 4827 1606
Size: 1151 677 1148 245 2120
Fragments arranged by size:
2120 1151 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2571 513
Size: 552 939 567 3283
Fragments arranged by size:
3283 939 567 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5341
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5341
NotI GC'GGCC_GC
Cuts at: 2788 2788
Size: 5341
NspI r_CATG'y
Cuts at: 1132 1229 2699 4244 1132
Size: 97 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5341
PciI A'CATG_T
Cuts at: 4240 4240
Size: 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2584 1108
Size: 1476 3865
PmeI GTTT'AAAC
Cuts at: 2645 2645
Size: 5341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 4923 4955 3410
Size: 32 1481 32 3796
Fragments arranged by size:
3796 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5341
PsiI TTA'TAA
Cuts at: 2911 2911
Size: 5341
PspOMI G'GGCC_C
Cuts at: 2399 2399
Size: 5341
PspXI vC'TCGA_Gb
Cuts at: 2477 2477
Size: 5341
PstI C_TGCA'G
Cuts at: 830 2693 830
Size: 1863 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5341
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 5341
SalI G'TCGA_C
Cuts at: 1936 2683 1936
Size: 747 4594
SbfI CC_TGCA'GG
Cuts at: 2693 2693
Size: 5341
ScaI AGT'ACT
Cuts at: 2360 3589 5301 2360
Size: 1229 1712 2400
Fragments arranged by size:
2400 1712 1229
SexAI A'CCwGG_T
Cuts at: 2348 2348
Size: 5341
SfcI C'TryA_G
Cuts at: 826 1045 2486 2689 3824 4505 4696 826
Size: 219 1441 203 1135 681 191 1471
Fragments arranged by size:
1471 1441 1135 681 219 203 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5341
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5341
SmaI CCC'GGG
Cuts at: 1872 2342 2411 2668 1872
Size: 470 69 257 4545
Fragments arranged by size:
4545 470 257 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 2477 3445 4346 4608 820
Size: 197 926 354 180 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 354 262 197 180
SnaBI TAC'GTA
Cuts at: 493 3132 493
Size: 2639 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5341
SphI G_CATG'C
Cuts at: 1132 2699 1132
Size: 1567 3774
SspI AAT'ATT
Cuts at: 5 52 3265 5
Size: 47 3213 2081
Fragments arranged by size:
3213 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2170 2571 2780 513
Size: 552 939 157 9 401 209 3074
Fragments arranged by size:
3074 939 552 401 209 157 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 3385 3538 3723 4168 325
Size: 1255 1788 17 153 185 445 1498
Fragments arranged by size:
1788 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2358 2580 3587
Size: 274 80 33 51 1443 381 222 1007
Cuts at: 3587 5299 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1007 381 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5301 5333 5301
Size: 32 5309
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5341
XbaI T'CTAG_A
Cuts at: 2677 2677
Size: 5341
XhoI C'TCGA_G
Cuts at: 1943 2477 1943
Size: 534 4807
XmaI C'CCGG_G
Cuts at: 1870 2340 2409 2666 1870
Size: 470 69 257 4545
Fragments arranged by size:
4545 470 257 69
XmnI GAAnn'nnTTC
Cuts at: 3470 3470
Size: 5341
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3149 276
Size: 53 83 186 936 1615 2468
Fragments arranged by size:
2468 1615 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI