Restriction map for
pFN21AB7566
(Circular) (Six-base) MAPSORT of: pFN21AB7566.seq Check: 4046 from: 1 to: 5536
pFN21AB7566 5536 bp
With 182 enzymes: SgfI *
March 28, 2012 12:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2087 3346 278
Size: 53 83 186 936 551 1259 2468
Fragments arranged by size:
2468 1259 936 551 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2879 1937
Size: 942 4594
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2699 3599 4983 5251 1632
Size: 1067 900 1384 268 1917
Fragments arranged by size:
1917 1384 1067 900 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 3279 4851 3279
Size: 1572 3964
ApaI G_GGCC'C
Cuts at: 2289 2531 2289
Size: 242 5294
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2845 3071 3223 5332 1493
Size: 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 226 152
AscI GG'CGCG_CC
Cuts at: 2376 2376
Size: 5536
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2165 2470 2861 1870
Size: 73 77 145 305 391 4545
Fragments arranged by size:
4545 391 305 145 77 73
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2340 2857 4312 618
Size: 325 217 627 341 212 517 1455 1842
Fragments arranged by size:
1842 1455 627 517 341 325 217 212
BanII G_rGCy'C
Cuts at: 1391 2259 2289 2531 2855 1391
Size: 868 30 242 324 4072
Fragments arranged by size:
4072 868 324 242 30
BbeI G_GCGC'C
Cuts at: 2132 2344 2132
Size: 212 5324
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 928
Size: 971 188 4377
Fragments arranged by size:
4377 971 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2300 2811
Size: 107 122 71 631 605 379 249 511
Cuts at: 2811 4147 136
Size: 1336 1525
Fragments arranged by size:
1525 1336 631 605 511 379 249 122
107 71
BglII A'GATC_T
Cuts at: 2481 5531 2481
Size: 3050 2486
BlpI GC'TnA_GC
Cuts at: 2036 2953 2036
Size: 917 4619
Bme1580I G_kGCm'C
Cuts at: 1790 2047 2289 2531 3597 4753 1790
Size: 257 242 242 1066 1156 2573
Fragments arranged by size:
2573 1156 1066 257 242 242
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2404 2513 4196 1239
Size: 1165 109 1683 2579
Fragments arranged by size:
2579 1683 1165 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 3661 4526 4824 2753
Size: 908 865 298 3465
Fragments arranged by size:
3465 908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 882
Size: 617 2700 2219
Fragments arranged by size:
2700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 2129 2341 2424
Size: 53 83 186 936 551 45 212 83
Cuts at: 2424 2639 2667 2829 3343 3725 4360 275
Size: 215 28 162 514 382 635 1451
Fragments arranged by size:
1451 936 635 551 514 382 215 212
186 162 83 83 53 45 28
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2624 2654 1178
Size: 30 165 30 1221 30 4060
Fragments arranged by size:
4060 1221 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5536
BseYI C'CCAG_C
Cuts at: 2137 2557 4739 5235 2137
Size: 420 2182 496 2438
Fragments arranged by size:
2438 2182 496 420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2390 1163
Size: 573 309 345 4309
Fragments arranged by size:
4309 573 345 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2118 2295 2418 2986 3747 4775
Size: 275 186 117 177 123 568 761 1028
Cuts at: 4775 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 568 275 186 177 123
117
BsiHKAI G_wGCw'C
Cuts at: 3597 3682 4753 3597
Size: 85 1071 4380
Fragments arranged by size:
4380 1071 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
BsmBI CGTCTCn'nnnn_
Cuts at: 2549 2549
Size: 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2259 2289 2531 2855 3597 3682
Size: 399 257 212 30 242 324 742 85
Cuts at: 3682 4753 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 257 242 212
85 30
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 844
Size: 2047 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 3423 1394
Size: 429 341 1259 3507
Fragments arranged by size:
3507 1259 429 341
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 2199 2270 2417 2721 4180 4367
Size: 110 307 82 71 147 304 1459 187
Cuts at: 4367 1700
Size: 2869
Fragments arranged by size:
2869 1459 307 304 187 147 110 82
71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 2151 2303 2376 2536 2538 1925
Size: 226 152 73 160 2 4923
Fragments arranged by size:
4923 226 160 152 73 2
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2521 2323
Size: 198 5338
BstYI r'GATC_y
Cuts at: 1972 2481 2866 3233 3618 3635 5087 5173 5185
Size: 509 385 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 509 385 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2205 2289 513
Size: 552 939 201 84 3760
Fragments arranged by size:
3760 939 552 201 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 2047 3052 3846 3866 2047
Size: 1005 794 20 3717
Fragments arranged by size:
3717 1005 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2070 2115
Size: 54 1425 50 275 18 222 18 45
Cuts at: 2115 2292 2325 2415 2643 2983 3872 5501 8
Size: 177 33 90 228 340 889 1629 43
Fragments arranged by size:
1629 1425 889 340 275 228 222 177
90 54 50 45 43 33 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2115 2292 2415 2983 1537
Size: 275 303 177 123 568 4090
Fragments arranged by size:
4090 568 303 275 177 123
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 1353
Size: 2113 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 2645 4123 4497 4643 387
Size: 1064 138 668 388 1478 374 146 1280
Fragments arranged by size:
1478 1280 1064 668 388 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2404 2513 2699 3599 4196 4983 5251
Size: 393 772 109 186 900 597 787 268
Cuts at: 5251 1239
Size: 1524
Fragments arranged by size:
1524 900 787 772 597 393 268 186
109
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 5536
EcoO109I rG'GnC_Cy
Cuts at: 2143 2169 2224 2389 2143
Size: 26 55 165 5290
Fragments arranged by size:
5290 165 55 26
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FseI GG_CCGG'CC
Cuts at: 2203 2203
Size: 5536
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 2132 2344 2798 4683 1678
Size: 454 212 454 1885 2531
Fragments arranged by size:
2531 1885 454 454 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2723 2755 4177
Size: 32 135 32 1052 32 67 32 1422
Cuts at: 4177 4209 4251 4283 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 1052 135 67 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KasI G'GCGC_C
Cuts at: 2128 2340 2128
Size: 212 5324
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4650 4834 1305
Size: 3345 184 2007
Fragments arranged by size:
3345 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3714
Fragments arranged by size:
3714 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3494 3853 4012 518
Size: 648 2328 359 159 2042
Fragments arranged by size:
2328 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2291 2297 2952 3629 4777 5022 1606
Size: 444 241 6 655 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 655 444 245 241 6
NaeI GCC'GGC
Cuts at: 2119 2201 2272 2419 2119
Size: 82 71 147 5236
Fragments arranged by size:
5236 147 82 71
NarI GG'CG_CC
Cuts at: 2129 2341 2129
Size: 212 5324
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4045
Fragments arranged by size:
4045 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NgoMIV G'CCGG_C
Cuts at: 2117 2199 2270 2417 2117
Size: 82 71 147 5236
Fragments arranged by size:
5236 147 82 71
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2292 2983 2292
Size: 691 4845
NspI r_CATG'y
Cuts at: 1132 1229 2894 4439 1132
Size: 97 1665 1545 2229
Fragments arranged by size:
2229 1665 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5536
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2355 1108
Size: 1247 4289
PfoI T'CCnGG_A
Cuts at: 2427 2427
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2169 2389 2169
Size: 220 5316
PshAI GACnn'nnGTC
Cuts at: 1613 2394 1613
Size: 781 4755
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PspOMI G'GGCC_C
Cuts at: 2285 2527 2285
Size: 242 5294
PstI C_TGCA'G
Cuts at: 830 2109 2888 830
Size: 1279 779 3478
Fragments arranged by size:
3478 1279 779
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
SacII CC_GC'GG
Cuts at: 2292 2292
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SanDI GG'GwC_CC
Cuts at: 2169 2389 2169
Size: 220 5316
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2105 2884 4019 4700 4891 826
Size: 219 1060 779 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 779 681 219 191
SfoI GGC'GCC
Cuts at: 2130 2342 2130
Size: 212 5324
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 2270 1700
Size: 570 4966
SmaI CCC'GGG
Cuts at: 1872 2022 2863 1872
Size: 150 841 4545
Fragments arranged by size:
4545 841 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 3640 4541 4803 820
Size: 197 926 825 872 901 262 1553
Fragments arranged by size:
1553 926 901 872 825 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SrfI GCCC'GGGC
Cuts at: 2022 2022
Size: 5536
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5536
StyI C'CwwG_G
Cuts at: 513 1065 2004 2832 2975 513
Size: 552 939 828 143 3074
Fragments arranged by size:
3074 939 828 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 3563 3580 3733 3918 4363 325
Size: 1255 1002 981 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1002 981 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3782 5494 96
Size: 274 80 33 51 1443 1805 1712 138
Fragments arranged by size:
1805 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2020 2861 1870
Size: 150 841 4545
Fragments arranged by size:
4545 841 150
XmnI GAAnn'nnTTC
Cuts at: 2501 3665 2501
Size: 1164 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2085 3344 276
Size: 53 83 186 936 551 1259 2468
Fragments arranged by size:
2468 1259 936 551 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SapI SexAI SfiI SwaI XcmI