Restriction map for
pFN21AB7565
(Circular) (Six-base) MAPSORT of: pFN21AB7565.seq Check: 8624 from: 1 to: 5332
pFN21AB7565 5332 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3142 278
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 1937
Size: 738 4594
Acc65I G'GTAC_C
Cuts at: 2653 2653
Size: 5332
AclI AA'CG_TT
Cuts at: 1563 3459 3832 1563
Size: 1896 373 3063
Fragments arranged by size:
3063 1896 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3395 4779 5047 1632
Size: 1763 1384 268 1917
Fragments arranged by size:
1917 1763 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5135
AflIII A'CryG_T
Cuts at: 1203 4231 5208 1203
Size: 3028 977 1327
Fragments arranged by size:
3028 1327 977
AgeI A'CCGG_T
Cuts at: 4163 4163
Size: 5332
AhdI GACnn_n'nnGTC
Cuts at: 4061 4061
Size: 5332
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5332
AlwNI CAG_nnn'CTG
Cuts at: 3075 4647 3075
Size: 1572 3760
ApaI G_GGCC'C
Cuts at: 2528 2528
Size: 5332
ApaLI G'TGCA_C
Cuts at: 3389 4545 3389
Size: 1156 4176
ApoI r'AATT_y
Cuts at: 1493 2123 2641 2867 3019 5128 1493
Size: 630 518 226 152 2109 1697
Fragments arranged by size:
2109 1697 630 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3886 160
Size: 872 2854 1606
Fragments arranged by size:
2854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
AvaI C'yCGr_G
Cuts at: 1870 1943 2657 1870
Size: 73 714 4545
Fragments arranged by size:
4545 714 73
BamHI G'GATC_C
Cuts at: 2662 3029 2662
Size: 367 4965
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2653 4108 618
Size: 325 217 627 866 1455 1842
Fragments arranged by size:
1842 1455 866 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2528 2651 1391
Size: 1137 123 4072
Fragments arranged by size:
4072 1137 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 928
Size: 971 260 4101
Fragments arranged by size:
4101 971 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 1903
Size: 34 1586 34 3678
Fragments arranged by size:
3678 1586 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2368 3224 4434 2368
Size: 856 1210 3266
Fragments arranged by size:
3266 1210 856
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5332
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3943 136
Size: 107 122 71 631 605 2271 1525
Fragments arranged by size:
2271 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5327 5327
Size: 5332
BlpI GC'TnA_GC
Cuts at: 2451 2749 2451
Size: 298 5034
Bme1580I G_kGCm'C
Cuts at: 1790 2528 3393 4549 1790
Size: 738 865 1156 2573
Fragments arranged by size:
2573 1156 865 738
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3063 4021 442
Size: 416 53 83 399 24 1646 958 1753
Fragments arranged by size:
1753 1646 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3992 1239
Size: 2753 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3457 4322 4620 3457
Size: 865 298 4169
Fragments arranged by size:
4169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2010 3995 882
Size: 617 511 1985 2219
Fragments arranged by size:
2219 1985 617 511
BsaAI yAC'GTr
Cuts at: 493 1204 3123 493
Size: 711 1919 2702
Fragments arranged by size:
2702 1919 711
BsaBI GATnn'nnATC
Cuts at: 5326 5326
Size: 5332
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3139 3521 4156 275
Size: 53 83 186 936 1606 382 635 1451
Fragments arranged by size:
1606 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3765 4163 4437 4584 4995 3765
Size: 398 274 147 411 4102
Fragments arranged by size:
4102 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5107
Fragments arranged by size:
5107 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 2591 2594 2597 2621 1186
Size: 1402 3 3 3 24 3897
Fragments arranged by size:
3897 1402 24 3 3 3
BseYI C'CCAG_C
Cuts at: 4535 5031 4535
Size: 496 4836
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 1163
Size: 573 348 4411
Fragments arranged by size:
4411 573 348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2782 3543 4571 1540
Size: 275 186 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 3393 3478 4549 2085
Size: 1308 85 1071 2868
Fragments arranged by size:
2868 1308 1071 85
BsiWI C'GTAC_G
Cuts at: 2049 2049
Size: 5332
BsmI GAATG_Cn'
Cuts at: 2843 2936 2843
Size: 93 5239
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2528 2651 3393 3478 4549 1391
Size: 399 295 443 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 295 123 85
BspEI T'CCGG_A
Cuts at: 4995 4995
Size: 5332
BspHI T'CATG_A
Cuts at: 3221 4951 3221
Size: 1730 3602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 2687 844
Size: 1234 609 3489
Fragments arranged by size:
3489 1234 609
BsrBI CCG'CTC
Cuts at: 1394 1823 3219 1394
Size: 429 1396 3507
Fragments arranged by size:
3507 1396 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3827 4001 65
Size: 1183 2579 174 1396
Fragments arranged by size:
2579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3976 4163 1700
Size: 110 2166 187 2869
Fragments arranged by size:
2869 2166 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5332
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5332
BssSI C'ACGA_G
Cuts at: 3392 4404 3392
Size: 1012 4320
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2492 794
Size: 1698 3634
BstBI TT'CG_AA
Cuts at: 4136 4136
Size: 5332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5332
BstYI r'GATC_y
Cuts at: 1972 2662 3029 3414 3431 4883 4969 4981 5327
Size: 690 367 385 17 1452 86 12 346
Cuts at: 5327 1972
Size: 1977
Fragments arranged by size:
1977 1452 690 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 513
Size: 552 939 26 3815
Fragments arranged by size:
3815 939 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2315 487
Size: 683 56 1089 3504
Fragments arranged by size:
3504 1089 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 3642 3662 2848
Size: 794 20 4518
Fragments arranged by size:
4518 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5297
DraI TTT'AAA
Cuts at: 2636 2636
Size: 5332
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 4339 809
Size: 507 792 2231 1802
Fragments arranged by size:
2231 1802 792 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2779 3668 5297
Size: 54 1425 50 275 18 949 889 1629
Cuts at: 5297 8
Size: 43
Fragments arranged by size:
1629 1425 949 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2779 1537
Size: 275 967 4090
Fragments arranged by size:
4090 967 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2110 2607 3262 1353
Size: 720 37 497 655 3423
Fragments arranged by size:
3423 720 655 497 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3919 4293 4439 387
Size: 1064 138 2330 374 146 1280
Fragments arranged by size:
2330 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3395 3992 4779 5047 1239
Size: 393 1763 597 787 268 1524
Fragments arranged by size:
1763 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2247 2247
Size: 5332
EcoO109I rG'GnC_Cy
Cuts at: 2275 2524 2525 2275
Size: 249 1 5082
Fragments arranged by size:
5082 249 1
EcoRI G'AATT_C
Cuts at: 2123 2641 3019 2123
Size: 518 378 4436
Fragments arranged by size:
4436 518 378
FspI TGC'GCA
Cuts at: 3838 3838
Size: 5332
HaeII r_GCGC'y
Cuts at: 1678 4479 1678
Size: 2801 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3973 4005 4047 4079 1373
Size: 32 135 32 2401 32 42 32 2626
Fragments arranged by size:
2626 2401 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2676 2922 669
Size: 988 281 738 246 3079
Fragments arranged by size:
3079 988 738 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5332
HpaI GTT'AAC
Cuts at: 2922 2922
Size: 5332
KpnI G_GTAC'C
Cuts at: 2657 2657
Size: 5332
MfeI C'AATT_G
Cuts at: 2931 2931
Size: 5332
MluI A'CGCG_T
Cuts at: 5208 5208
Size: 5332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4446 4630 1305
Size: 3141 184 2007
Fragments arranged by size:
3141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3510
Fragments arranged by size:
3510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3290 3649 3808 518
Size: 648 2124 359 159 2042
Fragments arranged by size:
2124 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2748 3425 4573 4818 1606
Size: 426 716 677 1148 245 2120
Fragments arranged by size:
2120 1148 716 677 426 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3841
Fragments arranged by size:
3841 939 552
NdeI CA'TA_TG
Cuts at: 387 2434 387
Size: 2047 3285
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5332
NotI GC'GGCC_GC
Cuts at: 2779 2779
Size: 5332
NspI r_CATG'y
Cuts at: 1132 1229 2690 4235 1132
Size: 97 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5332
PciI A'CATG_T
Cuts at: 4231 4231
Size: 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5332
PmeI GTTT'AAAC
Cuts at: 2636 2636
Size: 5332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 4914 4946 3401
Size: 32 1481 32 3787
Fragments arranged by size:
3787 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2275
Size: 5332
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5332
PsiI TTA'TAA
Cuts at: 2902 2902
Size: 5332
PspOMI G'GGCC_C
Cuts at: 2524 2524
Size: 5332
PstI C_TGCA'G
Cuts at: 830 2684 830
Size: 1854 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5332
SacII CC_GC'GG
Cuts at: 2033 2033
Size: 5332
SalI G'TCGA_C
Cuts at: 1936 2674 1936
Size: 738 4594
SapI GCTCTTCn'nnn_
Cuts at: 2073 2073
Size: 5332
SbfI CC_TGCA'GG
Cuts at: 2684 2684
Size: 5332
ScaI AGT'ACT
Cuts at: 3580 5292 3580
Size: 1712 3620
SfcI C'TryA_G
Cuts at: 826 1045 2680 3815 4496 4687 826
Size: 219 1635 1135 681 191 1471
Fragments arranged by size:
1635 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5332
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5332
SmaI CCC'GGG
Cuts at: 1872 2659 1872
Size: 787 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3436 4337 4599 820
Size: 197 926 1493 901 262 1553
Fragments arranged by size:
1553 1493 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3123 493
Size: 2630 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5332
SphI G_CATG'C
Cuts at: 1132 2690 1132
Size: 1558 3774
SspI AAT'ATT
Cuts at: 5 52 3256 5
Size: 47 3204 2081
Fragments arranged by size:
3204 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5332
StyI C'CwwG_G
Cuts at: 513 1065 2004 2496 2771 513
Size: 552 939 492 275 3074
Fragments arranged by size:
3074 939 552 492 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 3359 3376 3529 3714 4159 325
Size: 1255 801 978 17 153 185 445 1498
Fragments arranged by size:
1498 1255 978 801 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3578 5290 96
Size: 274 80 33 51 1443 1601 1712 138
Fragments arranged by size:
1712 1601 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5292 5324 5292
Size: 32 5300
Tth111I GACn'n_nGTC
Cuts at: 1316 2158 1316
Size: 842 4490
XbaI T'CTAG_A
Cuts at: 2668 2668
Size: 5332
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5332
XmaI C'CCGG_G
Cuts at: 1870 2657 1870
Size: 787 4545
XmnI GAAnn'nnTTC
Cuts at: 3461 3461
Size: 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3140 276
Size: 53 83 186 936 1606 2468
Fragments arranged by size:
2468 1606 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI XcmI