Restriction map for
pFN21AB7564
(Circular) (Six-base) MAPSORT of: pFN21AB7564.seq Check: 5841 from: 1 to: 5530
pFN21AB7564 5530 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 2606 3593 4977 5245 1632
Size: 878 96 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 878 268 96
AfeI AGC'GCT
Cuts at: 2564 2564
Size: 5530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 4429 5406 1203
Size: 3226 977 1327
Fragments arranged by size:
3226 1327 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 2364 1166
Size: 1198 4332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2631 1184
Size: 1447 4083
AlwNI CAG_nnn'CTG
Cuts at: 3273 4845 3273
Size: 1572 3958
ApaI G_GGCC'C
Cuts at: 2273 2675 2273
Size: 402 5128
ApaLI G'TGCA_C
Cuts at: 2449 3587 4743 2449
Size: 1138 1156 3236
Fragments arranged by size:
3236 1156 1138
ApoI r'AATT_y
Cuts at: 1493 2839 3065 3217 5326 1493
Size: 1346 226 152 2109 1697
Fragments arranged by size:
2109 1697 1346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2234 2249 2855 1870
Size: 73 192 99 15 606 4545
Fragments arranged by size:
4545 606 192 99 73 15
AvrII C'CTAG_G
Cuts at: 2110 2110
Size: 5530
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4306 618
Size: 325 217 627 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2256 2273 2675 2849 1391
Size: 679 186 17 402 174 4072
Fragments arranged by size:
4072 679 402 186 174 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2008 928
Size: 971 109 4450
Fragments arranged by size:
4450 971 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3721 3755 1903
Size: 34 50 34 1700 34 3678
Fragments arranged by size:
3678 1700 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5525 5525
Size: 5530
BlpI GC'TnA_GC
Cuts at: 2947 2947
Size: 5530
Bme1580I G_kGCm'C
Cuts at: 1790 2273 2453 2675 2713 3591 4747 1790
Size: 483 180 222 38 878 1156 2573
Fragments arranged by size:
2573 1156 878 483 222 180 38
BmgBI CAC'GTC
Cuts at: 1318 2500 1318
Size: 1182 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2096 3261 4219
Size: 416 53 83 399 24 679 1165 958
Cuts at: 4219 442
Size: 1753
Fragments arranged by size:
1753 1165 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 2594 4190 1239
Size: 981 374 1596 2579
Fragments arranged by size:
2579 1596 981 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2402 3655 4520 4818 2402
Size: 1253 865 298 3114
Fragments arranged by size:
3114 1253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2050 4193 882
Size: 617 551 2143 2219
Fragments arranged by size:
2219 2143 617 551
BsaAI yAC'GTr
Cuts at: 493 1204 2340 2701 3321 493
Size: 711 1136 361 620 2702
Fragments arranged by size:
2702 1136 711 620 361
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 3337 3719 4354
Size: 53 83 186 936 729 1075 382 635
Cuts at: 4354 275
Size: 1451
Fragments arranged by size:
1451 1075 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3963 4361 4635 4782 5193 3963
Size: 398 274 147 411 4300
Fragments arranged by size:
4300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 1178
Size: 30 165 30 1272 30 4003
Fragments arranged by size:
4003 1272 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2047 2217 2828 2831 2834 1186
Size: 858 3 170 611 3 3 3882
Fragments arranged by size:
3882 858 611 170 3 3 3
BseYI C'CCAG_C
Cuts at: 2058 2559 4733 5229 2058
Size: 501 2174 496 2359
Fragments arranged by size:
2359 2174 501 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2362 2624 2681 1163
Size: 573 626 262 57 4012
Fragments arranged by size:
4012 626 573 262 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2307 2980 3741 4769 1540
Size: 275 186 306 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 306 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2453 3591 3676 4747 2070
Size: 383 1138 85 1071 2853
Fragments arranged by size:
2853 1138 1071 383 85
BsmI GAATG_Cn'
Cuts at: 2517 3041 3134 2517
Size: 524 93 4913
Fragments arranged by size:
4913 524 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2256 2273 2453 2675 2713 2849
Size: 399 280 186 17 180 222 38 136
Cuts at: 2849 3591 3676 4747 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 280 222 186 180
136 85 38 17
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 3419 5149 3419
Size: 1730 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2703 2885 844
Size: 1859 182 3489
Fragments arranged by size:
3489 1859 182
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4025 4199 65
Size: 1183 2777 174 1396
Fragments arranged by size:
2777 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4174 4361 1700
Size: 110 2364 187 2869
Fragments arranged by size:
2869 2364 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5530
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 3590 4602 3590
Size: 1012 4518
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 2457 2541 794
Size: 1607 56 84 3783
Fragments arranged by size:
3783 1607 84 56
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525
Size: 888 367 385 17 1452 86 12 346
Cuts at: 5525 1972
Size: 1977
Fragments arranged by size:
1977 1452 888 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2596 513
Size: 552 1531 3447
Fragments arranged by size:
3447 1531 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4791
Fragments arranged by size:
4791 683 56
BtsI GCAGTG_nn'
Cuts at: 3046 3840 3860 3046
Size: 794 20 4716
Fragments arranged by size:
4716 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5495
DraI TTT'AAA
Cuts at: 2834 2834
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 809
Size: 507 3221 1802
Fragments arranged by size:
3221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2977 3866 5495
Size: 54 1425 50 275 18 1147 889 1629
Cuts at: 5495 8
Size: 43
Fragments arranged by size:
1629 1425 1147 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2977 1537
Size: 275 1165 4090
Fragments arranged by size:
4090 1165 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2486 3460 1353
Size: 668 465 974 3423
Fragments arranged by size:
3423 974 668 465
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4117 4491 4637 387
Size: 1064 138 2528 374 146 1280
Fragments arranged by size:
2528 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2068
Size: 5530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2510 2594 2606 3593 4190 4977
Size: 393 588 290 84 12 987 597 787
Cuts at: 4977 5245 1239
Size: 268 1524
Fragments arranged by size:
1524 987 787 597 588 393 290 268
84 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2810 2765
Size: 45 5485
EcoO109I rG'GnC_Cy
Cuts at: 2269 2270 2672 2686 2269
Size: 1 402 14 5113
Fragments arranged by size:
5113 402 14 1
EcoRI G'AATT_C
Cuts at: 2839 3217 2839
Size: 378 5152
FspI TGC'GCA
Cuts at: 4036 4036
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 2302 2566 4677 1678
Size: 624 264 2111 2531
Fragments arranged by size:
2531 2111 624 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 4171 4203 4245
Size: 32 135 32 732 32 1835 32 42
Cuts at: 4245 4277 1373
Size: 32 2626
Fragments arranged by size:
2626 1835 732 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2874 3120 669
Size: 988 281 936 246 3079
Fragments arranged by size:
3079 988 936 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5530
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4644 4828 1305
Size: 3339 184 2007
Fragments arranged by size:
3339 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3708
Fragments arranged by size:
3708 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2364 3488 3847 4006 518
Size: 648 936 262 1124 359 159 2042
Fragments arranged by size:
2042 1124 936 648 359 262 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2169 2175 2364 2629 2946 3623 4771
Size: 452 111 6 189 265 317 677 1148
Cuts at: 4771 5016 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 452 317 265 245 189
111 6
NcoI C'CATG_G
Cuts at: 513 1065 2596 513
Size: 552 1531 3447
Fragments arranged by size:
3447 1531 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2404 2888 4433 1132
Size: 97 1175 484 1545 2229
Fragments arranged by size:
2229 1545 1175 484 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5530
PciI A'CATG_T
Cuts at: 4429 4429
Size: 5530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5530
PfoI T'CCnGG_A
Cuts at: 2070 2070
Size: 5530
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PmlI CAC'GTG
Cuts at: 2340 2701 2340
Size: 361 5169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2686
Size: 5530
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PspOMI G'GGCC_C
Cuts at: 2269 2671 2269
Size: 402 5128
PstI C_TGCA'G
Cuts at: 830 2397 2769 2882 830
Size: 1567 372 113 3478
Fragments arranged by size:
3478 1567 372 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
PvuII CAG'CTG
Cuts at: 2058 2364 2058
Size: 306 5224
RsrII CG'GwC_CG
Cuts at: 2050 2050
Size: 5530
SacI G_AGCT'C
Cuts at: 2070 2070
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 3778 5490 3778
Size: 1712 3818
SexAI A'CCwGG_T
Cuts at: 2430 2430
Size: 5530
SfcI C'TryA_G
Cuts at: 826 1045 2393 2765 2878 4013 4694 4885 826
Size: 219 1348 372 113 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 681 372 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2857 1872
Size: 985 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2417 3634 4535 4797 820
Size: 197 926 474 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 474 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2404 2888 1132
Size: 1272 484 3774
Fragments arranged by size:
3774 1272 484
SspI AAT'ATT
Cuts at: 5 52 3454 5
Size: 47 3402 2081
Fragments arranged by size:
3402 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2110 2596 2969 513
Size: 552 1045 486 373 3074
Fragments arranged by size:
3074 1045 552 486 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2573 2693 3557 3574 3727 3912
Size: 1255 458 535 120 864 17 153 185
Cuts at: 3912 4357 325
Size: 445 1498
Fragments arranged by size:
1498 1255 864 535 458 445 185 153
120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3776 5488 96
Size: 274 80 33 51 1443 1799 1712 138
Fragments arranged by size:
1799 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5490 5522 5490
Size: 32 5498
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5530
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2017
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2855 1870
Size: 985 4545
XmnI GAAnn'nnTTC
Cuts at: 3659 3659
Size: 5530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI SacII SanDI SapI SfiI SfoI
SrfI SwaI