Restriction map for
pFN21AB7562
(Circular) (Six-base) MAPSORT of: pFN21AB7562.seq Check: 9738 from: 1 to: 5527
pFN21AB7562 5527 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3590 4974 5242 1632
Size: 458 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 2013 1166
Size: 847 4680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2045 2745 1184
Size: 861 700 3966
Fragments arranged by size:
3966 861 700
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 2490
Size: 32 5495
AlwNI CAG_nnn'CTG
Cuts at: 2200 3270 4842 2200
Size: 1070 1572 2885
Fragments arranged by size:
2885 1572 1070
ApaI G_GGCC'C
Cuts at: 2330 2330
Size: 5527
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2383 2630 2852 1870
Size: 73 440 247 222 4545
Fragments arranged by size:
4545 440 247 222 73
BamHI G'GATC_C
Cuts at: 2767 2857 3224 2767
Size: 90 367 5070
Fragments arranged by size:
5070 367 90
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2848 4303 618
Size: 325 217 627 1061 1455 1842
Fragments arranged by size:
1842 1455 1061 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2292 2322 2330 2846 1391
Size: 901 30 8 516 4072
Fragments arranged by size:
4072 901 516 30 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 928
Size: 971 660 3896
Fragments arranged by size:
3896 971 660
BbvCI CC'TCA_GC
Cuts at: 2313 2313
Size: 5527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3718 3752 1903
Size: 34 50 34 1697 34 3678
Fragments arranged by size:
3678 1697 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2213 4138 136
Size: 107 122 71 631 605 541 1925 1525
Fragments arranged by size:
1925 1525 631 605 541 122 107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2278 2403 2944 2278
Size: 125 541 4861
Fragments arranged by size:
4861 541 125
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2330 2459 2587 3588 4744 1790
Size: 468 72 129 128 1001 1156 2573
Fragments arranged by size:
2573 1156 1001 468 129 128 72
BmgBI CAC'GTC
Cuts at: 1318 2705 1318
Size: 1387 4140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2268 2387 2400 2435 2573 2657 4187 1239
Size: 1029 119 13 35 138 84 1530 2579
Fragments arranged by size:
2579 1530 1029 138 119 84 35 13
Bpu10I CC'TnA_GC
Cuts at: 2313 2313
Size: 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2799 3652 4517 4815 2799
Size: 853 865 298 3511
Fragments arranged by size:
3511 865 853 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2239 3318 493
Size: 711 1035 1079 2702
Fragments arranged by size:
2702 1079 1035 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3334 3716 4351
Size: 53 83 186 936 600 1201 382 635
Cuts at: 4351 275
Size: 1451
Fragments arranged by size:
1451 1201 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2602 2632 1178
Size: 30 165 30 777 30 392 30 4073
Fragments arranged by size:
4073 777 392 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2797 1186
Size: 1252 359 3916
Fragments arranged by size:
3916 1252 359
BseYI C'CCAG_C
Cuts at: 2097 2301 2398 2441 2519 2594 2659 2771 4730
Size: 204 97 43 78 75 65 112 1959
Cuts at: 4730 5226 2097
Size: 496 2398
Fragments arranged by size:
2398 1959 496 204 112 97 78 75
65 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2219 1163
Size: 573 387 96 4471
Fragments arranged by size:
4471 573 387 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3588 3673 4744 2724
Size: 864 85 1071 3507
Fragments arranged by size:
3507 1071 864 85
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2292 2322 2330 2459 2587 2724
Size: 399 468 34 30 8 129 128 137
Cuts at: 2724 2846 3588 3673 4744 1391
Size: 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 137 129 128
122 85 34 30 8
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 2431 3416 5146 2431
Size: 985 1730 2812
Fragments arranged by size:
2812 1730 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2737 2882 844
Size: 1893 145 3489
Fragments arranged by size:
3489 1893 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2410 3414 1394
Size: 429 587 1004 3507
Fragments arranged by size:
3507 1004 587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2029 4022 4196 65
Size: 1183 781 1993 174 1396
Fragments arranged by size:
1993 1396 1183 781 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 4171 4358 1700
Size: 110 296 2065 187 2869
Fragments arranged by size:
2869 2065 296 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 2150 3587 4599 2150
Size: 1437 1012 3078
Fragments arranged by size:
3078 1437 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2190 2767 2857 3224 3609 3626 5078 5164
Size: 218 577 90 367 385 17 1452 86
Cuts at: 5164 5176 5522 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 577 385 367 346 218 90
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2259 513
Size: 552 1194 3781
Fragments arranged by size:
3781 1194 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3837 3857 3043
Size: 794 20 4713
Fragments arranged by size:
4713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2974 3863 5492
Size: 54 1425 50 275 18 1144 889 1629
Cuts at: 5492 8
Size: 43
Fragments arranged by size:
1629 1425 1144 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3457 1353
Size: 2104 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2268 2387 2400 2435 2573 2657
Size: 393 458 178 119 13 35 138 84
Cuts at: 2657 3590 4187 4974 5242 1239
Size: 933 597 787 268 1524
Fragments arranged by size:
1524 933 787 597 458 393 268 178
138 119 84 35 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 2183
Size: 5527
EcoO109I rG'GnC_Cy
Cuts at: 2327 2664 2327
Size: 337 5190
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2251 4674 1678
Size: 573 2423 2531
Fragments arranged by size:
2531 2423 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2180 2212 2255 2287 4168
Size: 32 135 32 608 32 43 32 1881
Cuts at: 4168 4200 4242 4274 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1881 608 135 43 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2013 2241 3485 3844 4003 518
Size: 648 847 228 1244 359 159 2042
Fragments arranged by size:
2042 1244 847 648 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2365 2486 2512 2943 3620 4768 5013
Size: 462 297 121 26 431 677 1148 245
Cuts at: 5013 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 462 431 297 245 121
26
NaeI GCC'GGC
Cuts at: 2108 2108
Size: 5527
NcoI C'CATG_G
Cuts at: 513 1065 2259 513
Size: 552 1194 3781
Fragments arranged by size:
3781 1194 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NgoMIV G'CCGG_C
Cuts at: 2106 2106
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2692 1348
Size: 1344 4183
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PmlI CAC'GTG
Cuts at: 2239 2239
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2664 2664
Size: 5527
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2326 2326
Size: 5527
PspXI vC'TCGA_Gb
Cuts at: 2630 2630
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2205 2562 2712 2879 830
Size: 1375 357 150 167 3478
Fragments arranged by size:
3478 1375 357 167 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
PvuII CAG'CTG
Cuts at: 2068 2365 2512 2068
Size: 297 147 5083
Fragments arranged by size:
5083 297 147
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SbfI CC_TGCA'GG
Cuts at: 2205 2562 2879 2205
Size: 357 317 4853
Fragments arranged by size:
4853 357 317
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2201 2558 2708 2875 4010 4691 4882
Size: 219 1156 357 150 167 1135 681 191
Cuts at: 4882 826
Size: 1471
Fragments arranged by size:
1471 1156 1135 681 357 219 191 167
150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2385 2854 1872
Size: 513 469 4545
Fragments arranged by size:
4545 513 469
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2630 2814 3631 4532 4794 820
Size: 197 926 687 184 817 901 262 1553
Fragments arranged by size:
1553 926 901 817 687 262 197 184
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2049 2259 2476 2966 513
Size: 552 984 210 217 490 3074
Fragments arranged by size:
3074 984 552 490 217 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3773 5485 96
Size: 274 80 33 51 1443 1796 1712 138
Fragments arranged by size:
1796 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 5487 5519 2534
Size: 32 2921 32 2542
Fragments arranged by size:
2921 2542 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2656 2656
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 2630 1943
Size: 687 4840
XmaI C'CCGG_G
Cuts at: 1870 2383 2852 1870
Size: 513 469 4545
Fragments arranged by size:
4545 513 469
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI