Restriction map for
pFN21AB7558
(Circular) (Six-base) MAPSORT of: pFN21AB7558.seq Check: 9760 from: 1 to: 5518
pFN21AB7558 5518 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 2381 2435 2544 3581 4965 5233 1632
Size: 612 137 54 109 1037 1384 268 1917
Fragments arranged by size:
1917 1384 1037 612 268 137 109 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 3261 4833 3261
Size: 1572 3946
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2202 2257 2416 2827 3053 3205 5314 1493
Size: 709 55 159 411 226 152 2109 1697
Fragments arranged by size:
2109 1697 709 411 226 159 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2685 2843 1870
Size: 73 742 158 4545
Fragments arranged by size:
4545 742 158 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2008 2041 2008
Size: 33 5485
BamHI G'GATC_C
Cuts at: 2172 2848 3215 2172
Size: 676 367 4475
Fragments arranged by size:
4475 676 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 2839 4294 618
Size: 325 217 627 397 655 1455 1842
Fragments arranged by size:
1842 1455 655 627 397 325 217
BanII G_rGCy'C
Cuts at: 1391 2074 2837 1391
Size: 683 763 4072
Fragments arranged by size:
4072 763 683
BbeI G_GCGC'C
Cuts at: 2188 2188
Size: 5518
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2493 3579 4735 1790
Size: 703 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 703
BmgBI CAC'GTC
Cuts at: 1318 2031 1318
Size: 713 4805
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 4178 1239
Size: 1044 1895 2579
Fragments arranged by size:
2579 1895 1044
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2185 3325 3707 4342
Size: 53 83 186 936 652 1140 382 635
Cuts at: 4342 275
Size: 1451
Fragments arranged by size:
1451 1140 936 652 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2143 1186
Size: 949 8 4561
Fragments arranged by size:
4561 949 8
BseYI C'CCAG_C
Cuts at: 2492 4721 5217 2492
Size: 2229 496 2793
Fragments arranged by size:
2793 2229 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4945
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 3579 3664 4735 2074
Size: 1505 85 1071 2857
Fragments arranged by size:
2857 1505 1071 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
BsmBI CGTCTCn'nnnn_
Cuts at: 2241 2241
Size: 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2493 2837 3579 3664 4735 1391
Size: 399 284 419 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 399 344 284 85
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2337 4013 4187 65
Size: 1183 1089 1676 174 1396
Fragments arranged by size:
1676 1396 1183 1089 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4162 4349 1700
Size: 110 2352 187 2869
Fragments arranged by size:
2869 2352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2172 2544 2794 2848 3215 3600 3617 5069
Size: 200 372 250 54 367 385 17 1452
Cuts at: 5069 5155 5167 5513 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 372 367 346 250 200
86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2167 513
Size: 552 939 163 3864
Fragments arranged by size:
3864 939 552 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3828 3848 3034
Size: 794 20 4704
Fragments arranged by size:
4704 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483
Size: 54 1425 50 275 18 1135 889 1629
Cuts at: 5483 8
Size: 43
Fragments arranged by size:
1629 1425 1135 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2769 3448 1353
Size: 1416 679 3423
Fragments arranged by size:
3423 1416 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2072 2072
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2283 2381 2435 2544 3581 4178
Size: 393 612 39 98 54 109 1037 597
Cuts at: 4178 4965 5233 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1037 787 612 597 393 268 109
98 54 39
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2188 4665 1678
Size: 510 2477 2531
Fragments arranged by size:
2531 2477 510
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2581 2613 2727
Size: 32 135 32 457 32 520 32 114
Cuts at: 2727 2759 4159 4191 4233 4265 1373
Size: 32 1400 32 42 32 2626
Fragments arranged by size:
2626 1400 520 457 135 114 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KasI G'GCGC_C
Cuts at: 2184 2184
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 2642 3117 2642
Size: 475 5043
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2321 4632 4816 1305
Size: 858 158 2311 184 2007
Fragments arranged by size:
2311 2007 858 184 158
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3476 3835 3994 518
Size: 648 1119 1191 359 159 2042
Fragments arranged by size:
2042 1191 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2322 2934 3611 4759 5004 1606
Size: 716 612 677 1148 245 2120
Fragments arranged by size:
2120 1148 716 677 612 245
NarI GG'CG_CC
Cuts at: 2185 2185
Size: 5518
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2876 4421 1132
Size: 97 1647 1545 2229
Fragments arranged by size:
2229 1647 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PfoI T'CCnGG_A
Cuts at: 2745 2745
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
SacI G_AGCT'C
Cuts at: 2074 2074
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 2428 3766 5478 2428
Size: 1338 1712 2468
Fragments arranged by size:
2468 1712 1338
SexAI A'CCwGG_T
Cuts at: 2050 2050
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2866 4001 4682 4873 826
Size: 219 1821 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2186 2186
Size: 5518
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2685 3622 4523 4785 820
Size: 197 926 742 937 901 262 1553
Fragments arranged by size:
1553 937 926 901 742 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2957 513
Size: 552 939 953 3074
Fragments arranged by size:
3074 953 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 2426 3764
Size: 274 80 33 51 1443 409 40 1338
Cuts at: 3764 5476 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1338 409 274 138 80 51
40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2500 2500
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 2685 1943
Size: 742 4776
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SfiI SrfI SwaI