Restriction map for
pFN21AB7556
(Circular) (Six-base) MAPSORT of: pFN21AB7556.seq Check: 617 from: 1 to: 5518
pFN21AB7556 5518 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 2152 2627 3261 4833 2152
Size: 475 634 1572 2837
Fragments arranged by size:
2837 1572 634 475
ApaI G_GGCC'C
Cuts at: 2187 2260 2187
Size: 73 5445
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2843 1870
Size: 73 89 811 4545
Fragments arranged by size:
4545 811 89 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2567 2839 4294 618
Size: 325 217 627 329 451 272 1455 1842
Fragments arranged by size:
1842 1455 627 451 329 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2039 2187 2260 2837 1391
Size: 648 148 73 577 4072
Fragments arranged by size:
4072 648 577 148 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2698 928
Size: 971 799 3748
Fragments arranged by size:
3748 971 799
BbvCI CC'TCA_GC
Cuts at: 2740 2740
Size: 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2499 2533 3709 3743 1903
Size: 34 562 34 1176 34 3678
Fragments arranged by size:
3678 1176 562 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 136
Size: 107 122 71 631 605 2457 1525
Fragments arranged by size:
2457 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2260 3579 4735 1790
Size: 397 73 1319 1156 2573
Fragments arranged by size:
2573 1319 1156 397 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 4178 1239
Size: 1017 1922 2579
Fragments arranged by size:
2579 1922 1017
Bpu10I CC'TnA_GC
Cuts at: 2727 2740 2727
Size: 13 5505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2766 4181 882
Size: 617 1267 1415 2219
Fragments arranged by size:
2219 1415 1267 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3325 3707 4342 275
Size: 53 83 186 936 1792 382 635 1451
Fragments arranged by size:
1792 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2376 3951 4349 4623 4770 5181 2376
Size: 1575 398 274 147 411 2713
Fragments arranged by size:
2713 1575 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 1178
Size: 30 165 30 738 30 4525
Fragments arranged by size:
4525 738 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2474 2798 1186
Size: 1147 141 324 3906
Fragments arranged by size:
3906 1147 324 141
BseYI C'CCAG_C
Cuts at: 2097 2186 2696 2717 4721 5217 2097
Size: 89 510 21 2004 496 2398
Fragments arranged by size:
2398 2004 510 496 89 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 2633 1163
Size: 573 653 244 4048
Fragments arranged by size:
4048 653 573 244
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 2968 3729 4757 1540
Size: 275 186 81 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2039 3579 3664 4735 2039
Size: 1540 85 1071 2822
Fragments arranged by size:
2822 1540 1071 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2187 2260 2837 3579 3664 4735
Size: 399 249 148 73 577 742 85 1071
Cuts at: 4735 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 577 399 249 148 85
73
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 2664 3407 5137 2664
Size: 743 1730 3045
Fragments arranged by size:
3045 1730 743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 3405 1394
Size: 429 521 1061 3507
Fragments arranged by size:
3507 1061 521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4162 4349 1700
Size: 110 2352 187 2869
Fragments arranged by size:
2869 2352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2640 2640
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4966
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2732 487
Size: 683 56 1506 3273
Fragments arranged by size:
3273 1506 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2645 3034 3828 3848 2162
Size: 483 389 794 20 3832
Fragments arranged by size:
3832 794 483 389 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DraIII CAC_nnn'GTG
Cuts at: 2116 2116
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2240 2361
Size: 54 1425 50 275 18 249 161 121
Cuts at: 2361 2965 3854 5483 8
Size: 604 889 1629 43
Fragments arranged by size:
1629 1425 889 604 275 249 161 121
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 2965 1537
Size: 275 267 886 4090
Fragments arranged by size:
4090 886 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 3448 1353
Size: 957 1138 3423
Fragments arranged by size:
3423 1138 957
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2423 4105 4479 4625 387
Size: 1064 138 834 1682 374 146 1280
Fragments arranged by size:
1682 1280 1064 834 374 146 138
EcoICRI GAG'CTC
Cuts at: 2037 2037
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 3581 4178 4965 5233 1239
Size: 393 624 1325 597 787 268 1524
Fragments arranged by size:
1524 1325 787 624 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2183 2257 2624 2183
Size: 74 367 5077
Fragments arranged by size:
5077 367 74
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2326 2485 4665 1678
Size: 648 159 2180 2531
Fragments arranged by size:
2531 2180 648 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 2200 2232 2420
Size: 32 135 32 556 32 40 32 188
Cuts at: 2420 2452 2765 2797 4159 4191 4233 4265 1373
Size: 32 313 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1362 556 313 188 135 42 40
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2825 4632 4816 1305
Size: 1520 1807 184 2007
Fragments arranged by size:
2007 1807 1520 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 3476 3835 3994 518
Size: 648 1497 813 359 159 2042
Fragments arranged by size:
2042 1497 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2769 2934 3611 4759 5004 1606
Size: 1163 165 677 1148 245 2120
Fragments arranged by size:
2120 1163 1148 677 245 165
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4966
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2876 4421 1132
Size: 97 1647 1545 2229
Fragments arranged by size:
2229 1647 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2260 2621 1348
Size: 912 361 4245
Fragments arranged by size:
4245 912 361
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PfoI T'CCnGG_A
Cuts at: 2039 2526 2039
Size: 487 5031
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PspOMI G'GGCC_C
Cuts at: 2183 2256 2183
Size: 73 5445
PstI C_TGCA'G
Cuts at: 830 2157 2406 2448 2469 2870 830
Size: 1327 249 42 21 401 3478
Fragments arranged by size:
3478 1327 401 249 42 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
SacI G_AGCT'C
Cuts at: 2039 2039
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2406 2870 2406
Size: 464 5054
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2112 2112
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2153 2402 2444 2465 2866 4001 4682
Size: 219 1108 249 42 21 401 1135 681
Cuts at: 4682 4873 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1108 681 401 249 219 191
42 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 2203 2290 1517
Size: 686 87 4745
Fragments arranged by size:
4745 686 87
StyI C'CwwG_G
Cuts at: 513 1065 2538 2791 2957 513
Size: 552 1473 253 166 3074
Fragments arranged by size:
3074 1473 552 253 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 3545 3562 3715 3900 4345 325
Size: 1255 828 1137 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1137 828 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 3764 5476
Size: 274 80 33 51 1443 243 1544 1712
Cuts at: 5476 96
Size: 138
Fragments arranged by size:
1712 1544 1443 274 243 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 5478 5510 2141
Size: 32 3305 32 2149
Fragments arranged by size:
3305 2149 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI