Restriction map for
pFN21AB7552
(Circular) (Six-base) MAPSORT of: pFN21AB7552.seq Check: 4301 from: 1 to: 5509
pFN21AB7552 5509 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2852 1937
Size: 915 4594
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 3572 4956 5224 1632
Size: 914 1026 1384 268 1917
Fragments arranged by size:
1917 1384 1026 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 2806 4408 5385 1203
Size: 1603 1602 977 1327
Fragments arranged by size:
1603 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2475 1184
Size: 1291 4218
AlwNI CAG_nnn'CTG
Cuts at: 2595 3252 4824 2595
Size: 657 1572 3280
Fragments arranged by size:
3280 1572 657
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2818 3044 3196 5305 1493
Size: 1325 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2834 1870
Size: 73 891 4545
Fragments arranged by size:
4545 891 73
BamHI G'GATC_C
Cuts at: 2839 3206 2839
Size: 367 5142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2519 2830 4285 618
Size: 325 217 627 732 311 1455 1842
Fragments arranged by size:
1842 1455 732 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2695 2828 1391
Size: 1304 133 4072
Fragments arranged by size:
4072 1304 133
BbeI G_GCGC'C
Cuts at: 2523 2523
Size: 5509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4538
BbvCI CC'TCA_GC
Cuts at: 2147 2147
Size: 5509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3700 3734 1903
Size: 34 50 34 1679 34 3678
Fragments arranged by size:
3678 1679 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2231 3401 4611 2231
Size: 1170 1210 3129
Fragments arranged by size:
3129 1210 1170
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BfrBI ATG'CAT
Cuts at: 2533 2533
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2568 5504 2568
Size: 2936 2573
BlpI GC'TnA_GC
Cuts at: 2926 2926
Size: 5509
Bme1580I G_kGCm'C
Cuts at: 1790 3570 4726 1790
Size: 1780 1156 2573
Fragments arranged by size:
2573 1780 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3240 4198
Size: 416 53 83 399 24 1041 782 958
Cuts at: 4198 442
Size: 1753
Fragments arranged by size:
1753 1041 958 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2286 2131
Size: 155 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2740 4169 1239
Size: 1501 1429 2579
Fragments arranged by size:
2579 1501 1429
Bpu10I CC'TnA_GC
Cuts at: 2147 2152 2147
Size: 5 5504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3634 4499 4797 3634
Size: 865 298 4346
Fragments arranged by size:
4346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 3316 3698 4333
Size: 53 83 186 936 987 796 382 635
Cuts at: 4333 275
Size: 1451
Fragments arranged by size:
1451 987 936 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2234 3942 4340 4614 4761 5172 2234
Size: 1708 398 274 147 411 2571
Fragments arranged by size:
2571 1708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2176 2206 2273 2303 1178
Size: 30 165 30 773 30 67 30 4384
Fragments arranged by size:
4384 773 165 67 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2217 2233 2262 2278 1186
Size: 1031 16 29 16 4417
Fragments arranged by size:
4417 1031 29 16 16
BseYI C'CCAG_C
Cuts at: 2205 4712 5208 2205
Size: 2507 496 2506
Fragments arranged by size:
2507 2506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 2756 1163
Size: 573 699 321 3916
Fragments arranged by size:
3916 699 573 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 3720 4748 1540
Size: 275 186 958 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3570 3655 4726 3570
Size: 85 1071 4353
Fragments arranged by size:
4353 1071 85
BsmI GAATG_Cn'
Cuts at: 3020 3113 3020
Size: 93 5416
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2695 2828 3570 3655 4726 1391
Size: 399 905 133 742 85 1071 2174
Fragments arranged by size:
2174 1071 905 742 399 133 85
BspEI T'CCGG_A
Cuts at: 2234 5172 2234
Size: 2938 2571
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2864 844
Size: 2020 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4004 4178 65
Size: 1183 2756 174 1396
Fragments arranged by size:
2756 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4153 4340 1700
Size: 110 2343 187 2869
Fragments arranged by size:
2869 2343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 3569 4581 3569
Size: 1012 4497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2400 2571 2739 794
Size: 1606 171 168 3564
Fragments arranged by size:
3564 1606 171 168
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2142 2568 2839 3206 3591 3608 5060 5146
Size: 170 426 271 367 385 17 1452 86
Cuts at: 5146 5158 5504 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 426 385 367 346 271 170
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4957
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2472 487
Size: 683 56 1246 3524
Fragments arranged by size:
3524 1246 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 2653 3025 3819 3839 2452
Size: 201 372 794 20 4122
Fragments arranged by size:
4122 794 372 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DraIII CAC_nnn'GTG
Cuts at: 2178 2178
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2433 2956 3845
Size: 54 1425 50 275 18 603 523 889
Cuts at: 3845 5474 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 603 523 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 1537
Size: 275 1144 4090
Fragments arranged by size:
4090 1144 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 3439 1353
Size: 972 1114 3423
Fragments arranged by size:
3423 1114 972
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2740 3572 4169 4956 5224 1239
Size: 393 914 194 832 597 787 268 1524
Fragments arranged by size:
1524 914 832 787 597 393 268 194
EcoRI G'AATT_C
Cuts at: 2818 3196 2818
Size: 378 5131
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 2523 4656 1678
Size: 845 2133 2531
Fragments arranged by size:
2531 2133 845
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 2450 2482 2680
Size: 32 135 32 701 32 145 32 198
Cuts at: 2680 2712 4150 4182 4224 4256 1373
Size: 32 1438 32 42 32 2626
Fragments arranged by size:
2626 1438 701 198 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2853 3099 669
Size: 988 281 915 246 3079
Fragments arranged by size:
3079 988 915 281 246
HindIII A'AGCT_T
Cuts at: 748 2351 748
Size: 1603 3906
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KasI G'GCGC_C
Cuts at: 2519 2519
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4623 4807 1305
Size: 3318 184 2007
Fragments arranged by size:
3318 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 3084
Fragments arranged by size:
3084 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2457 2712 2778 3467 3826 3985
Size: 648 1008 283 255 66 689 359 159
Cuts at: 3985 518
Size: 2042
Fragments arranged by size:
2042 1008 689 648 359 283 255 159
66
MspA1I CmG'CkG
Cuts at: 1606 2189 2449 2484 2925 3602 4750 4995 1606
Size: 583 260 35 441 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 441 260 245 35
NarI GG'CG_CC
Cuts at: 2520 2520
Size: 5509
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4957
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NsiI A_TGCA'T
Cuts at: 2535 2535
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2867 4412 1132
Size: 97 1638 1545 2229
Fragments arranged by size:
2229 1638 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2595 1108
Size: 1487 4022
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 5091 5123 3578
Size: 32 1481 32 3964
Fragments arranged by size:
3964 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2344 1613
Size: 731 4778
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2703 2861 830
Size: 1873 158 3478
Fragments arranged by size:
3478 1873 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
PvuII CAG'CTG
Cuts at: 2449 2484 2449
Size: 35 5474
SalI G'TCGA_C
Cuts at: 1936 2851 1936
Size: 915 4594
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2059 2342 2699 2857 3992 4673 4864
Size: 219 1014 283 357 158 1135 681 191
Cuts at: 4864 826
Size: 1471
Fragments arranged by size:
1471 1135 1014 681 357 283 219 191
158
SfoI GGC'GCC
Cuts at: 2521 2521
Size: 5509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3613 4514 4776 820
Size: 197 926 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5509
StyI C'CwwG_G
Cuts at: 513 1065 2292 2948 513
Size: 552 1227 656 3074
Fragments arranged by size:
3074 1227 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 3553 3706 3891 4336 325
Size: 1255 1956 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3755 5467 96
Size: 274 80 33 51 1443 1778 1712 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5469 5501 5469
Size: 32 5477
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5509
XbaI T'CTAG_A
Cuts at: 2468 2845 2468
Size: 377 5132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2213 2213
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 2033 2385 3638 2033
Size: 352 1253 3904
Fragments arranged by size:
3904 1253 352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI