Restriction map for
pFN21AB7542
(Circular) (Six-base) MAPSORT of: pFN21AB7542.seq Check: 9950 from: 1 to: 5479
pFN21AB7542 5479 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2658 2658
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2675 3542 4926 5194 1632
Size: 725 318 867 1384 268 1917
Fragments arranged by size:
1917 1384 867 725 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 2127 2214 4378 5355 1203
Size: 924 87 2164 977 1327
Fragments arranged by size:
2164 1327 977 924 87
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 2549 1166
Size: 1383 4096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 2292
Size: 32 5447
AlwNI CAG_nnn'CTG
Cuts at: 3222 4794 3222
Size: 1572 3907
ApaLI G'TGCA_C
Cuts at: 3536 4692 3536
Size: 1156 4323
ApoI r'AATT_y
Cuts at: 1493 2150 2788 3014 3166 5275 1493
Size: 657 638 226 152 2109 1697
Fragments arranged by size:
2109 1697 657 638 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2804 1870
Size: 73 861 4545
Fragments arranged by size:
4545 861 73
BamHI G'GATC_C
Cuts at: 2236 2809 3176 2236
Size: 573 367 4539
Fragments arranged by size:
4539 573 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2800 4255 618
Size: 325 217 627 243 770 1455 1842
Fragments arranged by size:
1842 1455 770 627 325 243 217
BanII G_rGCy'C
Cuts at: 1391 2532 2798 1391
Size: 1141 266 4072
Fragments arranged by size:
4072 1141 266
BbeI G_GCGC'C
Cuts at: 2034 2034
Size: 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4508
BbvCI CC'TCA_GC
Cuts at: 2091 2754 2091
Size: 663 4816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2458 2492 3670 3704 1903
Size: 34 50 34 437 34 1178 34 3678
Fragments arranged by size:
3678 1178 437 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 2436 1548
Size: 888 4591
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4090 136
Size: 107 122 71 631 605 2418 1525
Fragments arranged by size:
2418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5474 5474
Size: 5479
BlpI GC'TnA_GC
Cuts at: 2526 2896 2526
Size: 370 5109
Bme1580I G_kGCm'C
Cuts at: 1790 3540 4696 1790
Size: 1750 1156 2573
Fragments arranged by size:
2573 1750 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5479
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2105 1056
Size: 1049 4430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2789
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 4139 1239
Size: 926 1974 2579
Fragments arranged by size:
2579 1974 926
Bpu10I CC'TnA_GC
Cuts at: 2091 2754 2091
Size: 663 4816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3604 4469 4767 3604
Size: 865 298 4316
Fragments arranged by size:
4316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 4142 882
Size: 617 1113 1530 2219
Fragments arranged by size:
2219 1530 1113 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 3270 493
Size: 711 1377 689 2702
Fragments arranged by size:
2702 1377 711 689
BsaBI GATnn'nnATC
Cuts at: 5473 5473
Size: 5479
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 3286 3668 4303
Size: 53 83 186 936 498 1255 382 635
Cuts at: 4303 275
Size: 1451
Fragments arranged by size:
1451 1255 936 635 498 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2178 3912 4310 4584 4731 5142 2178
Size: 1734 398 274 147 411 2515
Fragments arranged by size:
2515 1734 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2720 2750 1178
Size: 30 165 30 1317 30 3907
Fragments arranged by size:
3907 1317 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2771 1186
Size: 1585 3894
BseYI C'CCAG_C
Cuts at: 2275 4682 5178 2275
Size: 2407 496 2576
Fragments arranged by size:
2576 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 1163
Size: 573 486 4420
Fragments arranged by size:
4420 573 486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2532 3540 3625 4696 2532
Size: 1008 85 1071 3315
Fragments arranged by size:
3315 1071 1008 85
BsmI GAATG_Cn'
Cuts at: 2990 3083 2990
Size: 93 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2532 2798 3540 3625 4696 1391
Size: 399 742 266 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 742 399 266 85
BspEI T'CCGG_A
Cuts at: 2178 5142 2178
Size: 2964 2515
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2658 2834 844
Size: 1814 176 3489
Fragments arranged by size:
3489 1814 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 1394
Size: 429 1543 3507
Fragments arranged by size:
3507 1543 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 4310 1700
Size: 110 2313 187 2869
Fragments arranged by size:
2869 2313 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 3539 4551 3539
Size: 1012 4467
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2233
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2175 2236 2809 3176 3561 3578 5030 5116
Size: 203 61 573 367 385 17 1452 86
Cuts at: 5116 5128 5474 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 573 385 367 346 203 86
61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2014 2547 513
Size: 552 949 533 3445
Fragments arranged by size:
3445 949 552 533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2020 487
Size: 683 56 794 3946
Fragments arranged by size:
3946 794 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3789 3809 2995
Size: 794 20 4665
Fragments arranged by size:
4665 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DraIII CAC_nnn'GTG
Cuts at: 2548 2548
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444
Size: 54 1425 50 275 18 1096 889 1629
Cuts at: 5444 8
Size: 43
Fragments arranged by size:
1629 1425 1096 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 3409 1353
Size: 1373 683 3423
Fragments arranged by size:
3423 1373 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2530 2530
Size: 5479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2357 2675 3542 4139 4926 5194
Size: 393 533 192 318 867 597 787 268
Cuts at: 5194 1239
Size: 1524
Fragments arranged by size:
1524 867 787 597 533 393 318 268
192
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 2654
Size: 5479
EcoO109I rG'GnC_Cy
Cuts at: 2686 2686
Size: 5479
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2624 2624
Size: 5479
FspI TGC'GCA
Cuts at: 3985 3985
Size: 5479
HaeII r_GCGC'y
Cuts at: 1678 2034 2336 4626 1678
Size: 356 302 2290 2531
Fragments arranged by size:
2531 2290 356 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2720 2752 4120
Size: 32 135 32 495 32 621 32 1368
Cuts at: 4120 4152 4194 4226 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1368 621 495 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KasI G'GCGC_C
Cuts at: 2030 2030
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 4593 4777 1305
Size: 798 2490 184 2007
Fragments arranged by size:
2490 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3657
Fragments arranged by size:
3657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2346 2489 2549 3437 3796 3955
Size: 648 1065 115 143 60 888 359 159
Cuts at: 3955 518
Size: 2042
Fragments arranged by size:
2042 1065 888 648 359 159 143 115
60
MspA1I CmG'CkG
Cuts at: 1606 2016 2242 2525 2560 2753 2895 3572 4720
Size: 410 226 283 35 193 142 677 1148
Cuts at: 4720 4965 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 410 283 245 226 193
142 35
NarI GG'CG_CC
Cuts at: 2031 2031
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4927
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 2101 1052
Size: 1049 4430
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2131 2218 2837 4382 1132
Size: 97 902 87 619 1545 2229
Fragments arranged by size:
2229 1545 902 619 97 87
PasI CC'CwG_GG
Cuts at: 1348 2690 1348
Size: 1342 4137
PciI A'CATG_T
Cuts at: 2127 2214 4378 2127
Size: 87 2164 3228
Fragments arranged by size:
3228 2164 87
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5479
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5479
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2559 2831 830
Size: 1729 272 3478
Fragments arranged by size:
3478 1729 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2525 2560 2753 2525
Size: 35 193 5251
Fragments arranged by size:
5251 193 35
SacI G_AGCT'C
Cuts at: 2532 2532
Size: 5479
SacII CC_GC'GG
Cuts at: 2017 2017
Size: 5479
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SfcI C'TryA_G
Cuts at: 826 1045 2555 2827 3962 4643 4834 826
Size: 219 1510 272 1135 681 191 1471
Fragments arranged by size:
1510 1471 1135 681 272 219 191
SfoI GGC'GCC
Cuts at: 2032 2032
Size: 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5479
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3583 4484 4746 820
Size: 197 926 1640 901 262 1553
Fragments arranged by size:
1640 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5479
StyI C'CwwG_G
Cuts at: 513 1065 2918 513
Size: 552 1853 3074
Fragments arranged by size:
3074 1853 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 3506 3523 3676 3861 4306 325
Size: 1255 1056 870 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1056 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3725 5437 96
Size: 274 80 33 51 1443 1748 1712 138
Fragments arranged by size:
1748 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 5447
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SrfI SwaI XcmI