Restriction map for
pFN21AB7529
(Circular) (Six-base) MAPSORT of: pFN21AB7529.seq Check: 5631 from: 1 to: 5194
pFN21AB7529 5194 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2426 2426
Size: 5194
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3004 278
Size: 53 83 186 936 1468 2468
Fragments arranged by size:
2468 1468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2537 1937
Size: 600 4594
Acc65I G'GTAC_C
Cuts at: 2515 2515
Size: 5194
AclI AA'CG_TT
Cuts at: 1563 3321 3694 1563
Size: 1758 373 3063
Fragments arranged by size:
3063 1758 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2387 3257 4641 4909 1632
Size: 734 21 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 734 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4997
AflIII A'CryG_T
Cuts at: 1203 4093 5070 1203
Size: 2890 977 1327
Fragments arranged by size:
2890 1327 977
AgeI A'CCGG_T
Cuts at: 4025 4025
Size: 5194
AhdI GACnn_n'nnGTC
Cuts at: 3923 3923
Size: 5194
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2284 2486 1184
Size: 1100 202 3892
Fragments arranged by size:
3892 1100 202
AlwNI CAG_nnn'CTG
Cuts at: 2937 4509 2937
Size: 1572 3622
ApaLI G'TGCA_C
Cuts at: 3251 4407 3251
Size: 1156 4038
ApoI r'AATT_y
Cuts at: 1493 2503 2729 2881 4990 1493
Size: 1010 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3748 160
Size: 872 2716 1606
Fragments arranged by size:
2716 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5194
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2450 2474 2483 2519 1870
Size: 73 189 318 24 9 36 4545
Fragments arranged by size:
4545 318 189 73 36 24 9
BamHI G'GATC_C
Cuts at: 2524 2891 2524
Size: 367 4827
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2515 3970 618
Size: 325 217 627 448 280 1455 1842
Fragments arranged by size:
1842 1455 627 448 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2079 2513 1391
Size: 688 434 4072
Fragments arranged by size:
4072 688 434
BbeI G_GCGC'C
Cuts at: 2239 2239
Size: 5194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2354 928
Size: 971 455 3768
Fragments arranged by size:
3768 971 455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3385 3419 1903
Size: 34 1448 34 3678
Fragments arranged by size:
3678 1448 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3086 4296 3086
Size: 1210 3984
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5194
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3805 136
Size: 107 122 71 631 605 2133 1525
Fragments arranged by size:
2133 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5189 5189
Size: 5194
BlpI GC'TnA_GC
Cuts at: 2611 2611
Size: 5194
Bme1580I G_kGCm'C
Cuts at: 1790 3255 4411 1790
Size: 1465 1156 2573
Fragments arranged by size:
2573 1465 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5194
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2183 2925 3883
Size: 416 53 83 399 24 766 742 958
Cuts at: 3883 442
Size: 1753
Fragments arranged by size:
1753 958 766 742 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 3854 1239
Size: 944 1671 2579
Fragments arranged by size:
2579 1671 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3319 4184 4482 3319
Size: 865 298 4031
Fragments arranged by size:
4031 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3857 882
Size: 617 2358 2219
Fragments arranged by size:
2358 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2985 493
Size: 711 1781 2702
Fragments arranged by size:
2702 1781 711
BsaBI GATnn'nnATC
Cuts at: 5188 5188
Size: 5194
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2236 3001 3383 4018
Size: 53 83 186 936 703 765 382 635
Cuts at: 4018 275
Size: 1451
Fragments arranged by size:
1451 936 765 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2427 3627 4025 4299 4446 4857 2427
Size: 1200 398 274 147 411 2764
Fragments arranged by size:
2764 1200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4969
Fragments arranged by size:
4969 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2234 2375 1186
Size: 931 117 141 4005
Fragments arranged by size:
4005 931 141 117
BseYI C'CCAG_C
Cuts at: 2081 2187 4397 4893 2081
Size: 106 2210 496 2382
Fragments arranged by size:
2382 2210 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4621
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2644 3405 4433 1540
Size: 275 186 643 761 1028 2301
Fragments arranged by size:
2301 1028 761 643 275 186
BsiHKAI G_wGCw'C
Cuts at: 3255 3340 4411 3255
Size: 85 1071 4038
Fragments arranged by size:
4038 1071 85
BsmI GAATG_Cn'
Cuts at: 2705 2798 2705
Size: 93 5101
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2513 3255 3340 4411 1391
Size: 399 289 434 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 434 399 289 85
BspEI T'CCGG_A
Cuts at: 2427 4857 2427
Size: 2430 2764
BspHI T'CATG_A
Cuts at: 2331 3083 4813 2331
Size: 752 1730 2712
Fragments arranged by size:
2712 1730 752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2426 2549 844
Size: 1582 123 3489
Fragments arranged by size:
3489 1582 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 2377 3081 1394
Size: 429 497 57 704 3507
Fragments arranged by size:
3507 704 497 429 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 3689 3863 65
Size: 1183 2441 174 1396
Fragments arranged by size:
2441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3838 4025 1700
Size: 110 2028 187 2869
Fragments arranged by size:
2869 2028 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5194
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5194
BssSI C'ACGA_G
Cuts at: 3254 4266 3254
Size: 1012 4182
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5194
BstBI TT'CG_AA
Cuts at: 3998 3998
Size: 5194
BstEII G'GTnAC_C
Cuts at: 1184 2035 1184
Size: 851 4343
BstYI r'GATC_y
Cuts at: 1972 2019 2524 2891 3276 3293 4745 4831 4843
Size: 47 505 367 385 17 1452 86 12
Cuts at: 4843 5189 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 505 385 367 346 86 47
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4642
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4455
Fragments arranged by size:
4455 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2710 3504 3524 2468
Size: 242 794 20 4138
Fragments arranged by size:
4138 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5159
DraI TTT'AAA
Cuts at: 2498 2498
Size: 5194
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2452 4201 809
Size: 507 1136 1749 1802
Fragments arranged by size:
1802 1749 1136 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2641 3530 5159
Size: 54 1425 50 275 18 811 889 1629
Cuts at: 5159 8
Size: 43
Fragments arranged by size:
1629 1425 889 811 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2641 1537
Size: 275 829 4090
Fragments arranged by size:
4090 829 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 3124 1353
Size: 858 913 3423
Fragments arranged by size:
3423 913 858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3781 4155 4301 387
Size: 1064 138 2192 374 146 1280
Fragments arranged by size:
2192 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2366 2387 3257 3854 4641 4909
Size: 393 551 183 21 870 597 787 268
Cuts at: 4909 1239
Size: 1524
Fragments arranged by size:
1524 870 787 597 551 393 268 183
21
EcoO109I rG'GnC_Cy
Cuts at: 2184 2184
Size: 5194
EcoRI G'AATT_C
Cuts at: 2503 2881 2503
Size: 378 4816
FspI TGC'GCA
Cuts at: 3700 3700
Size: 5194
HaeII r_GCGC'y
Cuts at: 1678 2239 4341 1678
Size: 561 2102 2531
Fragments arranged by size:
2531 2102 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3835 3867 3909 3941 1373
Size: 32 135 32 2263 32 42 32 2626
Fragments arranged by size:
2626 2263 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2538 2784 669
Size: 988 281 600 246 3079
Fragments arranged by size:
3079 988 600 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5194
HpaI GTT'AAC
Cuts at: 2784 2784
Size: 5194
KasI G'GCGC_C
Cuts at: 2235 2235
Size: 5194
KpnI G_GTAC'C
Cuts at: 2519 2519
Size: 5194
MfeI C'AATT_G
Cuts at: 2793 2793
Size: 5194
MluI A'CGCG_T
Cuts at: 5070 5070
Size: 5194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4308 4492 1305
Size: 3003 184 2007
Fragments arranged by size:
3003 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3372
Fragments arranged by size:
3372 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 3152 3511 3670 518
Size: 648 1227 759 359 159 2042
Fragments arranged by size:
2042 1227 759 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2308 2610 3287 4435 4680 1606
Size: 702 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 702 677 302 245
NarI GG'CG_CC
Cuts at: 2236 2236
Size: 5194
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4642
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5194
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5194
NotI GC'GGCC_GC
Cuts at: 2641 2641
Size: 5194
NspI r_CATG'y
Cuts at: 1132 1229 2552 4097 1132
Size: 97 1323 1545 2229
Fragments arranged by size:
2229 1545 1323 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5194
PciI A'CATG_T
Cuts at: 4093 4093
Size: 5194
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2060 1108
Size: 952 4242
PfoI T'CCnGG_A
Cuts at: 2022 2160 2022
Size: 138 5056
PmeI GTTT'AAAC
Cuts at: 2498 2498
Size: 5194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 4776 4808 3263
Size: 32 1481 32 3649
Fragments arranged by size:
3649 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5194
PsiI TTA'TAA
Cuts at: 2764 2764
Size: 5194
PspXI vC'TCGA_Gb
Cuts at: 2474 2474
Size: 5194
PstI C_TGCA'G
Cuts at: 830 2307 2546 830
Size: 1477 239 3478
Fragments arranged by size:
3478 1477 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5194
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 5194
SalI G'TCGA_C
Cuts at: 1936 2536 1936
Size: 600 4594
SbfI CC_TGCA'GG
Cuts at: 2546 2546
Size: 5194
ScaI AGT'ACT
Cuts at: 3442 5154 3442
Size: 1712 3482
SfcI C'TryA_G
Cuts at: 826 1045 2303 2542 3677 4358 4549 826
Size: 219 1258 239 1135 681 191 1471
Fragments arranged by size:
1471 1258 1135 681 239 219 191
SfoI GGC'GCC
Cuts at: 2237 2237
Size: 5194
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5194
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5194
SmaI CCC'GGG
Cuts at: 1872 2521 1872
Size: 649 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 2483 3298 4199 4461 820
Size: 197 926 531 9 815 901 262 1553
Fragments arranged by size:
1553 926 901 815 531 262 197 9
SnaBI TAC'GTA
Cuts at: 493 2985 493
Size: 2492 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5194
SphI G_CATG'C
Cuts at: 1132 2552 1132
Size: 1420 3774
SspI AAT'ATT
Cuts at: 5 52 3118 5
Size: 47 3066 2081
Fragments arranged by size:
3066 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5194
StyI C'CwwG_G
Cuts at: 513 1065 2633 513
Size: 552 1568 3074
Fragments arranged by size:
3074 1568 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2447 3221 3238 3391 3576 4021 325
Size: 1255 867 774 17 153 185 445 1498
Fragments arranged by size:
1498 1255 867 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3440 5152 96
Size: 274 80 33 51 1443 1463 1712 138
Fragments arranged by size:
1712 1463 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2422 2454 5154 5186 2422
Size: 32 2700 32 2430
Fragments arranged by size:
2700 2430 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2438 1316
Size: 1122 4072
XbaI T'CTAG_A
Cuts at: 2530 2530
Size: 5194
XhoI C'TCGA_G
Cuts at: 1943 2474 2483 1943
Size: 531 9 4654
Fragments arranged by size:
4654 531 9
XmaI C'CCGG_G
Cuts at: 1870 2519 1870
Size: 649 4545
XmnI GAAnn'nnTTC
Cuts at: 3323 3323
Size: 5194
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3002 276
Size: 53 83 186 936 1468 2468
Fragments arranged by size:
2468 1468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI RsrII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI