Restriction map for
pFN21AB7526
(Circular) (Six-base) MAPSORT of: pFN21AB7526.seq Check: 1527 from: 1 to: 5458
pFN21AB7526 5458 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3268 278
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2801 1937
Size: 864 4594
Acc65I G'GTAC_C
Cuts at: 2779 2779
Size: 5458
AclI AA'CG_TT
Cuts at: 1563 3585 3958 1563
Size: 2022 373 3063
Fragments arranged by size:
3063 2022 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2411 2738 3521 4905 5173 1632
Size: 461 318 327 783 1384 268 1917
Fragments arranged by size:
1917 1384 783 461 327 318 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5261
AflIII A'CryG_T
Cuts at: 1203 4357 5334 1203
Size: 3154 977 1327
Fragments arranged by size:
3154 1327 977
AgeI A'CCGG_T
Cuts at: 4289 4289
Size: 5458
AhdI GACnn_n'nnGTC
Cuts at: 4187 4187
Size: 5458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5458
AlwNI CAG_nnn'CTG
Cuts at: 2071 2600 3201 4773 2071
Size: 529 601 1572 2756
Fragments arranged by size:
2756 1572 601 529
ApaI G_GGCC'C
Cuts at: 2652 2652
Size: 5458
ApaLI G'TGCA_C
Cuts at: 3515 4671 3515
Size: 1156 4302
ApoI r'AATT_y
Cuts at: 1493 2126 2169 2767 2993 3145 5254 1493
Size: 633 43 598 226 152 2109 1697
Fragments arranged by size:
2109 1697 633 598 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 2631 4012 160
Size: 872 1599 1381 1606
Fragments arranged by size:
1606 1599 1381 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
AvaI C'yCGr_G
Cuts at: 1870 1943 2783 1870
Size: 73 840 4545
Fragments arranged by size:
4545 840 73
BamHI G'GATC_C
Cuts at: 2788 3155 2788
Size: 367 5091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2779 4234 618
Size: 325 217 627 236 756 1455 1842
Fragments arranged by size:
1842 1455 756 627 325 236 217
BanII G_rGCy'C
Cuts at: 1391 2652 2777 1391
Size: 1261 125 4072
Fragments arranged by size:
4072 1261 125
BbeI G_GCGC'C
Cuts at: 2027 2027
Size: 5458
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3649 3683 1903
Size: 34 1712 34 3678
Fragments arranged by size:
3678 1712 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3350 4560 3350
Size: 1210 4248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4069 136
Size: 107 122 71 631 605 2397 1525
Fragments arranged by size:
2397 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5453 5453
Size: 5458
BlpI GC'TnA_GC
Cuts at: 2875 2875
Size: 5458
Bme1580I G_kGCm'C
Cuts at: 1790 2652 3519 4675 1790
Size: 862 867 1156 2573
Fragments arranged by size:
2573 1156 867 862
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5458
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3189 4147 442
Size: 416 53 83 399 24 1772 958 1753
Fragments arranged by size:
1772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4118 1239
Size: 2879 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4448 4746 3583
Size: 865 298 4295
Fragments arranged by size:
4295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4121 882
Size: 617 2622 2219
Fragments arranged by size:
2622 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3249 493
Size: 711 2045 2702
Fragments arranged by size:
2702 2045 711
BsaBI GATnn'nnATC
Cuts at: 5452 5452
Size: 5458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 3265 3647 4282
Size: 53 83 186 936 491 1241 382 635
Cuts at: 4282 275
Size: 1451
Fragments arranged by size:
1451 1241 936 635 491 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3891 4289 4563 4710 5121 3891
Size: 398 274 147 411 4228
Fragments arranged by size:
4228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2245 2275 1178
Size: 30 165 30 842 30 4361
Fragments arranged by size:
4361 842 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2250 1186
Size: 1064 4394
BseYI C'CCAG_C
Cuts at: 4661 5157 4661
Size: 496 4962
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 1163
Size: 573 843 4042
Fragments arranged by size:
4042 843 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3669 4697 1540
Size: 275 186 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3519 3604 4675 3519
Size: 85 1071 4302
Fragments arranged by size:
4302 1071 85
BsmI GAATG_Cn'
Cuts at: 2969 3062 2969
Size: 93 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2652 2777 3519 3604 4675 1391
Size: 399 862 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 862 742 399 125 85
BspEI T'CCGG_A
Cuts at: 5121 5121
Size: 5458
BspHI T'CATG_A
Cuts at: 3347 5077 3347
Size: 1730 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2813 844
Size: 1969 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3345 1394
Size: 429 1522 3507
Fragments arranged by size:
3507 1522 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3953 4127 65
Size: 1183 2705 174 1396
Fragments arranged by size:
2705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4102 4289 1700
Size: 110 2292 187 2869
Fragments arranged by size:
2869 2292 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5458
BssSI C'ACGA_G
Cuts at: 3518 4530 3518
Size: 1012 4446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2609 794
Size: 1815 3643
BstBI TT'CG_AA
Cuts at: 4262 4262
Size: 5458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5458
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 2677
Size: 5458
BstYI r'GATC_y
Cuts at: 1972 2067 2788 3155 3540 3557 5009 5095 5107
Size: 95 721 367 385 17 1452 86 12
Cuts at: 5107 5453 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 721 385 367 346 95 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4719
Fragments arranged by size:
4719 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3768 3788 2974
Size: 794 20 4644
Fragments arranged by size:
4644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5423
DraI TTT'AAA
Cuts at: 2762 2762
Size: 5458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4465 809
Size: 507 3149 1802
Fragments arranged by size:
3149 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2905 3794 5423
Size: 54 1425 50 275 18 1075 889 1629
Cuts at: 5423 8
Size: 43
Fragments arranged by size:
1629 1425 1075 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2905 1537
Size: 275 1093 4090
Fragments arranged by size:
4090 1093 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2714 3388 1353
Size: 1361 674 3423
Fragments arranged by size:
3423 1361 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4045 4419 4565 387
Size: 1064 138 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2411 2738 3521 4118 4905 5173
Size: 393 461 318 327 783 597 787 268
Cuts at: 5173 1239
Size: 1524
Fragments arranged by size:
1524 787 783 597 461 393 327 318
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2418 2418
Size: 5458
EcoO109I rG'GnC_Cy
Cuts at: 2339 2411 2339
Size: 72 5386
EcoRI G'AATT_C
Cuts at: 2767 3145 2767
Size: 378 5080
EcoRV GAT'ATC
Cuts at: 2287 2287
Size: 5458
FspI TGC'GCA
Cuts at: 3964 3964
Size: 5458
HaeII r_GCGC'y
Cuts at: 1678 2027 4605 1678
Size: 349 2578 2531
Fragments arranged by size:
2578 2531 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2011 2043 2211 2243 4099
Size: 32 135 32 439 32 168 32 1856
Cuts at: 4099 4131 4173 4205 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1856 439 168 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2802 3048 669
Size: 988 281 864 246 3079
Fragments arranged by size:
3079 988 864 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5458
HpaI GTT'AAC
Cuts at: 3048 3048
Size: 5458
KasI G'GCGC_C
Cuts at: 2023 2023
Size: 5458
KpnI G_GTAC'C
Cuts at: 2783 2783
Size: 5458
MfeI C'AATT_G
Cuts at: 3057 3057
Size: 5458
MluI A'CGCG_T
Cuts at: 5334 5334
Size: 5458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 4572 4756 1305
Size: 798 2469 184 2007
Fragments arranged by size:
2469 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3636
Fragments arranged by size:
3636 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2675 2733 3416 3775 3934 518
Size: 648 1509 58 683 359 159 2042
Fragments arranged by size:
2042 1509 683 648 359 159 58
MspA1I CmG'CkG
Cuts at: 1606 2874 3551 4699 4944 1606
Size: 1268 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 677 245
NarI GG'CG_CC
Cuts at: 2024 2024
Size: 5458
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3967
Fragments arranged by size:
3967 939 552
NdeI CA'TA_TG
Cuts at: 387 2332 387
Size: 1945 3513
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5458
NotI GC'GGCC_GC
Cuts at: 2905 2905
Size: 5458
NspI r_CATG'y
Cuts at: 1132 1229 2816 4361 1132
Size: 97 1587 1545 2229
Fragments arranged by size:
2229 1587 1545 97
PacI TTA_AT'TAA
Cuts at: 2403 2403
Size: 5458
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5458
PciI A'CATG_T
Cuts at: 4357 4357
Size: 5458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5458
PmeI GTTT'AAAC
Cuts at: 2762 2762
Size: 5458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 5040 5072 3527
Size: 32 1481 32 3913
Fragments arranged by size:
3913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2339 2411 2339
Size: 72 5386
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5458
PsiI TTA'TAA
Cuts at: 2111 3028 2111
Size: 917 4541
PspOMI G'GGCC_C
Cuts at: 2648 2648
Size: 5458
PstI C_TGCA'G
Cuts at: 830 2614 2810 830
Size: 1784 196 3478
Fragments arranged by size:
3478 1784 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5458
SalI G'TCGA_C
Cuts at: 1936 2800 1936
Size: 864 4594
SbfI CC_TGCA'GG
Cuts at: 2810 2810
Size: 5458
ScaI AGT'ACT
Cuts at: 3706 5418 3706
Size: 1712 3746
SfcI C'TryA_G
Cuts at: 826 1045 2383 2481 2610 2806 3941 4622 4813
Size: 219 1338 98 129 196 1135 681 191
Cuts at: 4813 826
Size: 1471
Fragments arranged by size:
1471 1338 1135 681 219 196 191 129
98
SfoI GGC'GCC
Cuts at: 2025 2025
Size: 5458
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5458
SmaI CCC'GGG
Cuts at: 1872 2785 1872
Size: 913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3562 4463 4725 820
Size: 197 926 1619 901 262 1553
Fragments arranged by size:
1619 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3249 493
Size: 2756 2702
SpeI A'CTAG_T
Cuts at: 152 2428 152
Size: 2276 3182
SphI G_CATG'C
Cuts at: 1132 2816 1132
Size: 1684 3774
SspI AAT'ATT
Cuts at: 5 52 2659 3382 5
Size: 47 2607 723 2081
Fragments arranged by size:
2607 2081 723 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5458
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2102 2419 2897 513
Size: 552 939 48 50 317 478 3074
Fragments arranged by size:
3074 939 552 478 317 50 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3485 3502 3655 3840 4285 325
Size: 1255 1905 17 153 185 445 1498
Fragments arranged by size:
1905 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 5416 96
Size: 274 80 33 51 1443 1727 1712 138
Fragments arranged by size:
1727 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5418 5450 5418
Size: 32 5426
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5458
XbaI T'CTAG_A
Cuts at: 2794 2794
Size: 5458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5458
XmaI C'CCGG_G
Cuts at: 1870 2783 1870
Size: 913 4545
XmnI GAAnn'nnTTC
Cuts at: 3587 3587
Size: 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3266 276
Size: 53 83 186 936 1732 2468
Fragments arranged by size:
2468 1732 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PacI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI NaeI NgoMIV NruI NsiI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI