Restriction map for
pFN21AB7514
(Circular) (Six-base) MAPSORT of: pFN21AB7514.seq Check: 149 from: 1 to: 5440
pFN21AB7514 5440 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3503 4887 5155 1632
Size: 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2696 1184
Size: 1512 3928
AlwNI CAG_nnn'CTG
Cuts at: 2198 2458 2724 3183 4755 2198
Size: 260 266 459 1572 2883
Fragments arranged by size:
2883 1572 459 266 260
ApaI G_GGCC'C
Cuts at: 2024 2174 2508 2024
Size: 150 334 4956
Fragments arranged by size:
4956 334 150
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2376 2765 1870
Size: 73 433 389 4545
Fragments arranged by size:
4545 433 389 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 4216 618
Size: 325 217 627 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2174 2449 2508 2759 1391
Size: 633 150 275 59 251 4072
Fragments arranged by size:
4072 633 275 251 150 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2645 928
Size: 971 746 3723
Fragments arranged by size:
3723 971 746
BbvCI CC'TCA_GC
Cuts at: 2013 2013
Size: 5440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BfrBI ATG'CAT
Cuts at: 2688 2688
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 4051 136
Size: 107 122 71 631 605 802 1577 1525
Fragments arranged by size:
1577 1525 802 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2301 2385 5435 2301
Size: 84 3050 2306
Fragments arranged by size:
3050 2306 84
BlpI GC'TnA_GC
Cuts at: 2084 2857 2084
Size: 773 4667
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2174 2508 3501 4657 1790
Size: 234 150 334 993 1156 2573
Fragments arranged by size:
2573 1156 993 334 234 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 4100 1239
Size: 1076 1785 2579
Fragments arranged by size:
2579 1785 1076
Bpu10I CC'TnA_GC
Cuts at: 2013 2013
Size: 5440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2553 3565 4430 4728 2553
Size: 1012 865 298 3265
Fragments arranged by size:
3265 1012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2511 3231 493
Size: 711 1307 720 2702
Fragments arranged by size:
2702 1307 720 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2245 2275 1178
Size: 30 165 30 842 30 4343
Fragments arranged by size:
4343 842 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2532 2603 1186
Size: 960 386 71 4023
Fragments arranged by size:
4023 960 386 71
BseYI C'CCAG_C
Cuts at: 2501 2617 4643 5139 2501
Size: 116 2026 496 2802
Fragments arranged by size:
2802 2026 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 1163
Size: 573 666 4201
Fragments arranged by size:
4201 666 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3501 3586 4657 3501
Size: 85 1071 4284
Fragments arranged by size:
4284 1071 85
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2174 2449 2508 2759 3501 3586
Size: 399 234 150 275 59 251 742 85
Cuts at: 3586 4657 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 275 251 234 150
85 59
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2795 844
Size: 1951 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3935 4109 65
Size: 1183 2687 174 1396
Fragments arranged by size:
2687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 2548 3500 4512 2548
Size: 952 1012 3476
Fragments arranged by size:
3476 1012 952
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 2491
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2301 2385 2770 3137 3522 3539 4991 5077
Size: 329 84 385 367 385 17 1452 86
Cuts at: 5077 5089 5435 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 385 367 346 329 86
84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2191 2191
Size: 5440
BtgI C'CryG_G
Cuts at: 513 1065 2627 513
Size: 552 1562 3326
Fragments arranged by size:
3326 1562 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 2956 3750 3770 2520
Size: 436 794 20 4190
Fragments arranged by size:
4190 794 436 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2887 3776 5405
Size: 54 1425 50 275 18 1057 889 1629
Cuts at: 5405 8
Size: 43
Fragments arranged by size:
1629 1425 1057 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 2237 3370 1353
Size: 807 77 1133 3423
Fragments arranged by size:
3423 1133 807 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2431 4027 4401 4547 387
Size: 1064 138 842 1596 374 146 1280
Fragments arranged by size:
1596 1280 1064 842 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 3503 4100 4887 5155 1239
Size: 393 683 1188 597 787 268 1524
Fragments arranged by size:
1524 1188 787 683 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2646 2646
Size: 5440
EcoO109I rG'GnC_Cy
Cuts at: 2021 2071 2170 2171 2021
Size: 50 99 1 5290
Fragments arranged by size:
5290 99 50 1
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 2531 3946 2531
Size: 1415 4025
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 4081 4113 4155
Size: 32 135 32 970 32 1507 32 42
Cuts at: 4155 4187 1373
Size: 32 2626
Fragments arranged by size:
2626 1507 970 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4554 4738 1305
Size: 3249 184 2007
Fragments arranged by size:
3249 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3618
Fragments arranged by size:
3618 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 3398 3757 3916 518
Size: 648 1512 720 359 159 2042
Fragments arranged by size:
2042 1512 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2284 2347 2458 2578 2856 3533 4681 4926
Size: 678 63 111 120 278 677 1148 245
Cuts at: 4926 1606
Size: 2120
Fragments arranged by size:
2120 1148 678 677 278 245 120 111
63
NcoI C'CATG_G
Cuts at: 513 1065 2627 513
Size: 552 1562 3326
Fragments arranged by size:
3326 1562 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NsiI A_TGCA'T
Cuts at: 2690 2690
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2798 4343 1132
Size: 97 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5440
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PmlI CAC'GTG
Cuts at: 2511 2511
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2020 2170 2504 2020
Size: 150 334 4956
Fragments arranged by size:
4956 334 150
PstI C_TGCA'G
Cuts at: 830 2036 2792 830
Size: 1206 756 3478
Fragments arranged by size:
3478 1206 756
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2284 2347 2458 2578 2284
Size: 63 111 120 5146
Fragments arranged by size:
5146 120 111 63
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2237
Size: 5440
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2032 2788 3923 4604 4795 826
Size: 219 987 756 1135 681 191 1471
Fragments arranged by size:
1471 1135 987 756 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2568 3544 4445 4707 820
Size: 197 926 625 976 901 262 1553
Fragments arranged by size:
1553 976 926 901 625 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 2122 1517
Size: 605 4835
StyI C'CwwG_G
Cuts at: 513 1065 2627 2879 513
Size: 552 1562 252 3074
Fragments arranged by size:
3074 1562 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2640 3467 3484 3637 3822 4267 325
Size: 1255 1060 827 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1060 827 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2628 2660 5400 5432 2628
Size: 32 2740 32 2636
Fragments arranged by size:
2740 2636 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2625 2709 2625
Size: 84 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 2303 3569 2303
Size: 1266 4174
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI