Restriction map for
pFN21AB7502
(Circular) (Six-base) MAPSORT of: pFN21AB7502.seq Check: 8914 from: 1 to: 5413
pFN21AB7502 5413 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3476 4860 5128 1632
Size: 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 3156 4728 3156
Size: 1572 3841
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2532 3967 160
Size: 872 1500 1435 1606
Fragments arranged by size:
1606 1500 1435 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2021 2054 2021
Size: 33 5380
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2359 2734 4189 618
Size: 325 217 627 572 375 1455 1842
Fragments arranged by size:
1842 1455 627 572 375 325 217
BanII G_rGCy'C
Cuts at: 1391 2732 1391
Size: 1341 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 928
Size: 971 284 4158
Fragments arranged by size:
4158 971 284
BbvCI CC'TCA_GC
Cuts at: 2508 2508
Size: 5413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3305 4515 3305
Size: 1210 4203
BclI T'GATC_A
Cuts at: 1548 2395 1548
Size: 847 4566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4024 136
Size: 107 122 71 631 605 2352 1525
Fragments arranged by size:
2352 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4102 442
Size: 416 53 83 399 24 1727 958 1753
Fragments arranged by size:
1753 1727 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 4073 1239
Size: 1149 1685 2579
Fragments arranged by size:
2579 1685 1149
Bpu10I CC'TnA_GC
Cuts at: 2508 2508
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3538 4403 4701 3538
Size: 865 298 4250
Fragments arranged by size:
4250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 2394 2517 5407 2394
Size: 123 2890 2400
Fragments arranged by size:
2890 2400 123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3220 3602 4237
Size: 53 83 186 936 476 1211 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 1211 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3846 4244 4518 4665 5076 3846
Size: 398 274 147 411 4183
Fragments arranged by size:
4183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2199 2229 2361 2391 1178
Size: 30 165 30 796 30 132 30 4200
Fragments arranged by size:
4200 796 165 132 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5413
BseYI C'CCAG_C
Cuts at: 2098 4616 5112 2098
Size: 2518 496 2399
Fragments arranged by size:
2518 2399 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 1163
Size: 573 791 4049
Fragments arranged by size:
4049 791 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3474 3559 4630 3474
Size: 85 1071 4257
Fragments arranged by size:
4257 1071 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2732 3474 3559 4630 1391
Size: 399 942 742 85 1071 2174
Fragments arranged by size:
2174 1071 942 742 399 85
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 2146 2461 3302 5032 2146
Size: 315 841 1730 2527
Fragments arranged by size:
2527 1730 841 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2558 2768 844
Size: 1714 210 3489
Fragments arranged by size:
3489 1714 210
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3908 4082 65
Size: 1183 2660 174 1396
Fragments arranged by size:
2660 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4057 4244 1700
Size: 110 2247 187 2869
Fragments arranged by size:
2869 2247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5413
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2743 3110 3495 3512 4964 5050 5062 5408
Size: 771 367 385 17 1452 86 12 346
Cuts at: 5408 1972
Size: 1977
Fragments arranged by size:
1977 1452 771 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2552 513
Size: 552 939 548 3374
Fragments arranged by size:
3374 939 552 548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 2929 3723 3743 2227
Size: 702 794 20 3897
Fragments arranged by size:
3897 794 702 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378
Size: 54 1425 50 275 18 1030 889 1629
Cuts at: 5378 8
Size: 43
Fragments arranged by size:
1629 1425 1030 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 2615 3343 1353
Size: 813 449 728 3423
Fragments arranged by size:
3423 813 728 449
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 3476 4073 4860 5128 1239
Size: 393 756 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 787 756 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2507 2507
Size: 5413
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 5413
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2476 4560 1678
Size: 798 2084 2531
Fragments arranged by size:
2531 2084 798
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4054 4086 4128 4160 1373
Size: 32 135 32 2482 32 42 32 2626
Fragments arranged by size:
2626 2482 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3003 669
Size: 988 281 819 246 3079
Fragments arranged by size:
3079 988 819 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 4527 4711 1305
Size: 880 2342 184 2007
Fragments arranged by size:
2342 2007 880 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2829 3506 4654 4899 1606
Size: 1223 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3922
Fragments arranged by size:
3922 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2569 2765 830
Size: 1739 196 3478
Fragments arranged by size:
3478 1739 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2565 2761 3896 4577 4768 826
Size: 219 1520 196 1135 681 191 1471
Fragments arranged by size:
1520 1471 1135 681 219 196 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3517 4418 4680 820
Size: 197 926 1574 901 262 1553
Fragments arranged by size:
1574 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2004 2852 513
Size: 552 939 848 3074
Fragments arranged by size:
3074 939 848 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2199 2231 2361 2393 5373 5405 2199
Size: 32 130 32 2980 32 2207
Fragments arranged by size:
2980 2207 130 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 3542 3542
Size: 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI