Restriction map for
pFN21AB7501
(Circular) (Six-base) MAPSORT of: pFN21AB7501.seq Check: 1094 from: 1 to: 4879
pFN21AB7501 4879 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2689 278
Size: 53 83 186 936 1153 2468
Fragments arranged by size:
2468 1153 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2222 1937
Size: 285 4594
Acc65I G'GTAC_C
Cuts at: 2200 2200
Size: 4879
AclI AA'CG_TT
Cuts at: 1563 3006 3379 1563
Size: 1443 373 3063
Fragments arranged by size:
3063 1443 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2942 4326 4594 1632
Size: 1310 1384 268 1917
Fragments arranged by size:
1917 1384 1310 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4682
AflIII A'CryG_T
Cuts at: 1203 3778 4755 1203
Size: 2575 977 1327
Fragments arranged by size:
2575 1327 977
AgeI A'CCGG_T
Cuts at: 3710 3710
Size: 4879
AhdI GACnn_n'nnGTC
Cuts at: 3608 3608
Size: 4879
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4879
AlwNI CAG_nnn'CTG
Cuts at: 2622 4194 2622
Size: 1572 3307
ApaLI G'TGCA_C
Cuts at: 2936 4092 2936
Size: 1156 3723
ApoI r'AATT_y
Cuts at: 1493 2188 2414 2566 4675 1493
Size: 695 226 152 2109 1697
Fragments arranged by size:
2109 1697 695 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3433 160
Size: 872 2401 1606
Fragments arranged by size:
2401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4879
AvaI C'yCGr_G
Cuts at: 1870 1943 2175 2204 1870
Size: 73 232 29 4545
Fragments arranged by size:
4545 232 73 29
BamHI G'GATC_C
Cuts at: 2098 2209 2576 2098
Size: 111 367 4401
Fragments arranged by size:
4401 367 111
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2200 3655 618
Size: 325 217 627 413 1455 1842
Fragments arranged by size:
1842 1455 627 413 325 217
BanII G_rGCy'C
Cuts at: 1391 2198 1391
Size: 807 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3070 3104 1903
Size: 34 1133 34 3678
Fragments arranged by size:
3678 1133 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2117 2771 3981 2117
Size: 654 1210 3015
Fragments arranged by size:
3015 1210 654
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4879
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3490 136
Size: 107 122 71 631 605 1818 1525
Fragments arranged by size:
1818 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4874 4874
Size: 4879
BlpI GC'TnA_GC
Cuts at: 2296 2296
Size: 4879
Bme1580I G_kGCm'C
Cuts at: 1790 2940 4096 1790
Size: 1150 1156 2573
Fragments arranged by size:
2573 1156 1150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4879
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2610 3568 442
Size: 416 53 83 399 24 1193 958 1753
Fragments arranged by size:
1753 1193 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3539 1239
Size: 2300 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3004 3869 4167 3004
Size: 865 298 3716
Fragments arranged by size:
3716 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3542 882
Size: 617 2043 2219
Fragments arranged by size:
2219 2043 617
BsaAI yAC'GTr
Cuts at: 493 1204 2670 493
Size: 711 1466 2702
Fragments arranged by size:
2702 1466 711
BsaBI GATnn'nnATC
Cuts at: 2054 4873 2054
Size: 2819 2060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2686 3068 3703 275
Size: 53 83 186 936 1153 382 635 1451
Fragments arranged by size:
1451 1153 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3312 3710 3984 4131 4542 3312
Size: 398 274 147 411 3649
Fragments arranged by size:
3649 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4654
Fragments arranged by size:
4654 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4879
BseYI C'CCAG_C
Cuts at: 4082 4578 4082
Size: 496 4383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2329 3090 4118 1540
Size: 275 186 328 761 1028 2301
Fragments arranged by size:
2301 1028 761 328 275 186
BsiHKAI G_wGCw'C
Cuts at: 2940 3025 4096 2940
Size: 85 1071 3723
Fragments arranged by size:
3723 1071 85
BsmI GAATG_Cn'
Cuts at: 2390 2483 2390
Size: 93 4786
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2940 3025 4096 1391
Size: 399 408 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 85
BspEI T'CCGG_A
Cuts at: 4542 4542
Size: 4879
BspHI T'CATG_A
Cuts at: 2768 4498 2768
Size: 1730 3149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 844
Size: 1390 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2766 1394
Size: 429 943 3507
Fragments arranged by size:
3507 943 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3374 3548 65
Size: 1183 2126 174 1396
Fragments arranged by size:
2126 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3523 3710 1700
Size: 110 1713 187 2869
Fragments arranged by size:
2869 1713 187 110
BsrGI T'GTAC_A
Cuts at: 96 2136 96
Size: 2040 2839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4879
BssSI C'ACGA_G
Cuts at: 2939 3951 2939
Size: 1012 3867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4879
BstBI TT'CG_AA
Cuts at: 3683 3683
Size: 4879
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4879
BstYI r'GATC_y
Cuts at: 1972 2098 2209 2576 2961 2978 4430 4516 4528
Size: 126 111 367 385 17 1452 86 12
Cuts at: 4528 4874 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 126 111 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3388
Fragments arranged by size:
3388 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4140
Fragments arranged by size:
4140 683 56
BtsI GCAGTG_nn'
Cuts at: 2395 3189 3209 2395
Size: 794 20 4065
Fragments arranged by size:
4065 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4844
DraI TTT'AAA
Cuts at: 2183 2183
Size: 4879
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3886 809
Size: 507 2570 1802
Fragments arranged by size:
2570 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 3215 4844
Size: 54 1425 50 275 18 496 889 1629
Cuts at: 4844 8
Size: 43
Fragments arranged by size:
1629 1425 889 496 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2326 1537
Size: 275 514 4090
Fragments arranged by size:
4090 514 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2809 1353
Size: 1456 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3466 3840 3986 387
Size: 1064 138 1877 374 146 1280
Fragments arranged by size:
1877 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2942 3539 4326 4594 1239
Size: 393 1310 597 787 268 1524
Fragments arranged by size:
1524 1310 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2188 2566 2188
Size: 378 4501
FspI TGC'GCA
Cuts at: 3385 3385
Size: 4879
HaeII r_GCGC'y
Cuts at: 1678 4026 1678
Size: 2348 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3520 3552 3594 3626 1373
Size: 32 135 32 1948 32 42 32 2626
Fragments arranged by size:
2626 1948 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2223 2469 669
Size: 988 281 285 246 3079
Fragments arranged by size:
3079 988 285 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4879
HpaI GTT'AAC
Cuts at: 2469 2469
Size: 4879
KpnI G_GTAC'C
Cuts at: 2204 2204
Size: 4879
MfeI C'AATT_G
Cuts at: 2478 2478
Size: 4879
MluI A'CGCG_T
Cuts at: 4755 4755
Size: 4879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3993 4177 1305
Size: 2688 184 2007
Fragments arranged by size:
2688 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3057
Fragments arranged by size:
3057 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3196 3355 518
Size: 648 1671 359 159 2042
Fragments arranged by size:
2042 1671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2295 2972 4120 4365 1606
Size: 689 677 1148 245 2120
Fragments arranged by size:
2120 1148 689 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3388
Fragments arranged by size:
3388 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4879
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4879
NotI GC'GGCC_GC
Cuts at: 2326 2326
Size: 4879
NspI r_CATG'y
Cuts at: 1132 1229 2237 3782 1132
Size: 97 1008 1545 2229
Fragments arranged by size:
2229 1545 1008 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4879
PciI A'CATG_T
Cuts at: 3778 3778
Size: 4879
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4879
PmeI GTTT'AAAC
Cuts at: 2183 2183
Size: 4879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 4461 4493 2948
Size: 32 1481 32 3334
Fragments arranged by size:
3334 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4879
PsiI TTA'TAA
Cuts at: 2449 2449
Size: 4879
PspXI vC'TCGA_Gb
Cuts at: 2175 2175
Size: 4879
PstI C_TGCA'G
Cuts at: 830 2231 830
Size: 1401 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4879
SalI G'TCGA_C
Cuts at: 1936 2221 1936
Size: 285 4594
SbfI CC_TGCA'GG
Cuts at: 2231 2231
Size: 4879
ScaI AGT'ACT
Cuts at: 3127 4839 3127
Size: 1712 3167
SfcI C'TryA_G
Cuts at: 826 1045 2227 3362 4043 4234 826
Size: 219 1182 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4879
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4879
SmaI CCC'GGG
Cuts at: 1872 2206 1872
Size: 334 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 2983 3884 4146 820
Size: 197 926 232 808 901 262 1553
Fragments arranged by size:
1553 926 901 808 262 232 197
SnaBI TAC'GTA
Cuts at: 493 2670 493
Size: 2177 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4879
SphI G_CATG'C
Cuts at: 1132 2237 1132
Size: 1105 3774
SspI AAT'ATT
Cuts at: 5 52 2803 5
Size: 47 2751 2081
Fragments arranged by size:
2751 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4879
StyI C'CwwG_G
Cuts at: 513 1065 2004 2318 513
Size: 552 939 314 3074
Fragments arranged by size:
3074 939 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2906 2923 3076 3261 3706 325
Size: 1255 447 879 17 153 185 445 1498
Fragments arranged by size:
1498 1255 879 447 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 3125 4837
Size: 274 80 33 51 1443 159 989 1712
Cuts at: 4837 96
Size: 138
Fragments arranged by size:
1712 1443 989 274 159 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4839 4871 4839
Size: 32 4847
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4879
XbaI T'CTAG_A
Cuts at: 2215 2215
Size: 4879
XhoI C'TCGA_G
Cuts at: 1943 2175 1943
Size: 232 4647
XmaI C'CCGG_G
Cuts at: 1870 2204 1870
Size: 334 4545
XmnI GAAnn'nnTTC
Cuts at: 3008 3008
Size: 4879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2687 276
Size: 53 83 186 936 1153 2468
Fragments arranged by size:
2468 1153 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI