Restriction map for
pFN21AB7452
(Circular) (Six-base) MAPSORT of: pFN21AB7452.seq Check: 7779 from: 1 to: 5518
pFN21AB7452 5518 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2573 2573
Size: 5518
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2035 2839 2035
Size: 804 4714
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 3581 4965 5233 1632
Size: 551 1398 1384 268 1917
Fragments arranged by size:
1917 1398 1384 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 2278 2296 2617 3261 4833 2278
Size: 18 321 644 1572 2963
Fragments arranged by size:
2963 1572 644 321 18
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2183 2266 2474 2843 1870
Size: 73 139 101 83 208 369 4545
Fragments arranged by size:
4545 369 208 139 101 83 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2252 2313 2488 2530
Size: 325 217 627 248 217 61 175 42
Cuts at: 2530 2746 2839 4294 618
Size: 216 93 1455 1842
Fragments arranged by size:
1842 1455 627 325 248 217 217 216
175 93 61 42
BanII G_rGCy'C
Cuts at: 1391 2265 2837 1391
Size: 874 572 4072
Fragments arranged by size:
4072 874 572
BbeI G_GCGC'C
Cuts at: 2256 2492 2750 2256
Size: 236 258 5024
Fragments arranged by size:
5024 258 236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2536 928
Size: 971 637 3910
Fragments arranged by size:
3910 971 637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2089 3410 4620 2089
Size: 1321 1210 2987
Fragments arranged by size:
2987 1321 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2204 2463
Size: 107 122 71 631 605 337 195 259
Cuts at: 2463 2639 2655 4129 136
Size: 176 16 1474 1525
Fragments arranged by size:
1525 1474 631 605 337 259 195 176
122 107 71 16
BglII A'GATC_T
Cuts at: 5513 5513
Size: 5518
BlpI GC'TnA_GC
Cuts at: 2537 2935 2537
Size: 398 5120
Bme1580I G_kGCm'C
Cuts at: 1790 2558 3579 4735 1790
Size: 768 1021 1156 2573
Fragments arranged by size:
2573 1156 1021 768
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2600 2639 2699 2747 2792 4178 1239
Size: 953 408 39 60 48 45 1386 2579
Fragments arranged by size:
2579 1386 953 408 60 48 45 39
Bpu10I CC'TnA_GC
Cuts at: 2384 2444 2384
Size: 60 5458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 882
Size: 617 2682 2219
Fragments arranged by size:
2682 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2150 3309 493
Size: 711 946 1159 2702
Fragments arranged by size:
2702 1159 946 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2253 2489 2703 2747
Size: 53 83 186 936 720 236 214 44
Cuts at: 2747 3325 3707 4342 275
Size: 578 382 635 1451
Fragments arranged by size:
1451 936 720 635 578 382 236 214
186 83 53 44
BsaWI w'CCGG_w
Cuts at: 3951 4349 4623 4770 5181 3951
Size: 398 274 147 411 4288
Fragments arranged by size:
4288 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5293
Fragments arranged by size:
5293 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2201 2492 2777 1186
Size: 937 78 291 285 3927
Fragments arranged by size:
3927 937 291 285 78
BseYI C'CCAG_C
Cuts at: 4721 5217 4721
Size: 496 5022
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 1163
Size: 573 792 4153
Fragments arranged by size:
4153 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2068 2100 2196 2631 2647
Size: 275 186 48 19 32 96 435 16
Cuts at: 2647 2968 3729 4757 1540
Size: 321 761 1028 2301
Fragments arranged by size:
2301 1028 761 435 321 275 186 96
48 32 19 16
BsiHKAI G_wGCw'C
Cuts at: 3579 3664 4735 3579
Size: 85 1071 4362
Fragments arranged by size:
4362 1071 85
BsmI GAATG_Cn'
Cuts at: 3029 3122 3029
Size: 93 5425
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2558 2837 3579 3664 4735 1391
Size: 399 475 293 279 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 399 293 279 85
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 2573 2873 844
Size: 1272 457 300 3489
Fragments arranged by size:
3489 1272 457 300
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2048 2390 2597 2732 2809 4162
Size: 110 234 4 342 207 135 77 1353
Cuts at: 4162 4349 1700
Size: 187 2869
Fragments arranged by size:
2869 1353 342 234 207 187 135 110
77 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 2304 2591 2593 2601 2685 2717 1925
Size: 379 287 2 8 84 32 4726
Fragments arranged by size:
4726 379 287 84 32 8 2
BssSI C'ACGA_G
Cuts at: 2522 3578 4590 2522
Size: 1056 1012 3450
Fragments arranged by size:
3450 1056 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513
Size: 876 367 385 17 1452 86 12 346
Cuts at: 5513 1972
Size: 1977
Fragments arranged by size:
1977 1452 876 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2637 513
Size: 552 939 633 3394
Fragments arranged by size:
3394 939 633 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2779 487
Size: 683 56 1553 3226
Fragments arranged by size:
3226 1553 683 56
BtsI GCAGTG_nn'
Cuts at: 2019 2516 3034 3828 3848 2019
Size: 497 518 794 20 3689
Fragments arranged by size:
3689 794 518 497 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2065 2097
Size: 54 1425 50 275 18 216 19 32
Cuts at: 2097 2464 2628 2640 2965 3854 5483 8
Size: 367 164 12 325 889 1629 43
Fragments arranged by size:
1629 1425 889 367 325 275 216 164
54 50 43 32 19 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2065 2097 2628 2965 1537
Size: 275 234 19 32 531 337 4090
Fragments arranged by size:
4090 531 337 275 234 32 19
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 2612 2795 3448 1353
Size: 1046 213 183 653 3423
Fragments arranged by size:
3423 1046 653 213 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2192 2600 2639 2699 2747 2792
Size: 393 551 9 408 39 60 48 45
Cuts at: 2792 3581 4178 4965 5233 1239
Size: 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 597 551 408 393 268
60 48 45 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2162 2123
Size: 39 5479
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 2501 4024 2501
Size: 1523 3995
HaeII r_GCGC'y
Cuts at: 1678 2256 2296 2391 2492 2750 4665 1678
Size: 578 40 95 101 258 1915 2531
Fragments arranged by size:
2531 1915 578 258 101 95 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373
Size: 32 135 32 2587 32 42 32 2626
Fragments arranged by size:
2626 2587 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KasI G'GCGC_C
Cuts at: 2252 2488 2746 2252
Size: 236 258 5024
Fragments arranged by size:
5024 258 236
KpnI G_GTAC'C
Cuts at: 2039 2843 2039
Size: 804 4714
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 3835 3994 518
Size: 648 2310 359 159 2042
Fragments arranged by size:
2310 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2494 2536 2614 2934 3611 4759 5004
Size: 458 430 42 78 320 677 1148 245
Cuts at: 5004 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 458 430 320 245 78
42
NaeI GCC'GGC
Cuts at: 2046 2392 2599 2734 2046
Size: 346 207 135 4830
Fragments arranged by size:
4830 346 207 135
NarI GG'CG_CC
Cuts at: 2253 2489 2747 2253
Size: 236 258 5024
Fragments arranged by size:
5024 258 236
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2044 2390 2597 2732 2044
Size: 346 207 135 4830
Fragments arranged by size:
4830 346 207 135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2065 2965 2065
Size: 900 4618
NruI TCG'CGA
Cuts at: 2798 2798
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2876 4421 1132
Size: 97 1647 1545 2229
Fragments arranged by size:
2229 1647 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2617 1108
Size: 1509 4009
PfoI T'CCnGG_A
Cuts at: 2724 2724
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2286 2694 2870 830
Size: 1456 408 176 3478
Fragments arranged by size:
3478 1456 408 176
PvuI CG_AT'CG
Cuts at: 2001 2196 2001
Size: 195 5323
PvuII CAG'CTG
Cuts at: 2614 2614
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SapI GCTCTTCn'nnn_
Cuts at: 2612 2795 2612
Size: 183 5335
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SfcI C'TryA_G
Cuts at: 826 1045 2282 2431 2690 2866 4001 4682 4873
Size: 219 1237 149 259 176 1135 681 191
Cuts at: 4873 826
Size: 1471
Fragments arranged by size:
1471 1237 1135 681 259 219 191 176
149
SfoI GGC'GCC
Cuts at: 2254 2490 2748 2254
Size: 236 258 5024
Fragments arranged by size:
5024 258 236
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 2390 2597 1700
Size: 690 207 4621
Fragments arranged by size:
4621 690 207
SmaI CCC'GGG
Cuts at: 1872 2084 2268 2845 1872
Size: 212 184 577 4545
Fragments arranged by size:
4545 577 212 184
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2876 1132
Size: 1744 3774
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2957 513
Size: 552 939 953 3074
Fragments arranged by size:
3074 953 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5476 96
Size: 274 80 33 51 1443 1787 1712 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2082 2266 2843 1870
Size: 212 184 577 4545
Fragments arranged by size:
4545 577 212 184
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI