Restriction map for
pFN21AB7451
(Circular) (Six-base) MAPSORT of: pFN21AB7451.seq Check: 413 from: 1 to: 5953
pFN21AB7451 5953 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 2949 3296 1937
Size: 520 492 347 4594
Fragments arranged by size:
4594 520 492 347
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4016 5400 5668 1632
Size: 2384 1384 268 1917
Fragments arranged by size:
2384 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3053 820
Size: 197 2036 3720
Fragments arranged by size:
3720 2036 197
AflIII A'CryG_T
Cuts at: 1203 4852 5829 1203
Size: 3649 977 1327
Fragments arranged by size:
3649 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2840 1184
Size: 1656 4297
AlwNI CAG_nnn'CTG
Cuts at: 3696 5268 3696
Size: 1572 4381
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2983 3262 3488 3640 5749 1493
Size: 1490 279 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2545 3278 1870
Size: 73 602 733 4545
Fragments arranged by size:
4545 733 602 73
BamHI G'GATC_C
Cuts at: 2608 3130 3283 3650 2608
Size: 522 153 367 4911
Fragments arranged by size:
4911 522 367 153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2210 3274 4729 618
Size: 325 217 627 227 196 1064 1455 1842
Fragments arranged by size:
1842 1455 1064 627 325 227 217 196
BanII G_rGCy'C
Cuts at: 1391 2026 2250 2799 3272 1391
Size: 635 224 549 473 4072
Fragments arranged by size:
4072 635 549 473 224
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 5953
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 928
Size: 971 252 4730
Fragments arranged by size:
4730 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2279 2313 4144 4178 1903
Size: 34 50 34 258 34 1831 34 3678
Fragments arranged by size:
3678 1831 258 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5948 5948
Size: 5953
BlpI GC'TnA_GC
Cuts at: 2249 3370 2249
Size: 1121 4832
Bme1580I G_kGCm'C
Cuts at: 1790 2829 4014 5170 1790
Size: 1039 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 1039
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 4666
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2816 3684 4642
Size: 416 53 83 399 24 1399 868 958
Cuts at: 4642 442
Size: 1753
Fragments arranged by size:
1753 1399 958 868 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2348
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2777 4613 1239
Size: 1025 513 1836 2579
Fragments arranged by size:
2579 1836 1025 513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2128 2371 4078 4943 5241 2128
Size: 243 1707 865 298 2840
Fragments arranged by size:
2840 1707 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3121 3760 4142
Size: 53 83 186 936 482 1106 639 382
Cuts at: 4142 4777 275
Size: 635 1451
Fragments arranged by size:
1451 1106 936 639 635 482 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5728
Fragments arranged by size:
5728 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2140 2723 2726 2729 2732 1186
Size: 954 583 3 3 3 4407
Fragments arranged by size:
4407 954 583 3 3 3
BseYI C'CCAG_C
Cuts at: 2286 2656 5156 5652 2286
Size: 370 2500 496 2587
Fragments arranged by size:
2587 2500 496 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2314 3113 1163
Size: 573 329 249 799 4003
Fragments arranged by size:
4003 799 573 329 249
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 3403 4164 5192 1540
Size: 275 186 204 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 204 186
BsiHKAI G_wGCw'C
Cuts at: 2026 2250 2412 2799 4014 4099 5170 2026
Size: 224 162 387 1215 85 1071 2809
Fragments arranged by size:
2809 1215 1071 387 224 162 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
BsmBI CGTCTCn'nnnn_
Cuts at: 2998 2998
Size: 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2250 2412 2799 2829 3272 4014
Size: 399 236 224 162 387 30 443 742
Cuts at: 4014 4099 5170 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 387 236 224
162 85 30
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2547 3308 844
Size: 1703 761 3489
Fragments arranged by size:
3489 1703 761
BsrBI CCG'CTC
Cuts at: 1394 1823 3174 3840 1394
Size: 429 1351 666 3507
Fragments arranged by size:
3507 1351 666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 2213 4597 4784 1700
Size: 110 397 6 2384 187 2869
Fragments arranged by size:
2869 2384 397 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 2019 4013 5025 2019
Size: 1994 1012 2947
Fragments arranged by size:
2947 1994 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2476 794
Size: 1682 4271
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2608 3130 3283 3650 4035 4052 5504 5590
Size: 636 522 153 367 385 17 1452 86
Cuts at: 5590 5602 5948 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 636 522 385 367 346 153
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3792
Fragments arranged by size:
3792 1609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2297 2696 2774 3469 4263 4283 2297
Size: 399 78 695 794 20 3967
Fragments arranged by size:
3967 794 695 399 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2590 2598
Size: 54 1425 50 275 18 372 388 8
Cuts at: 2598 2677 3400 4289 5918 8
Size: 79 723 889 1629 43
Fragments arranged by size:
1629 1425 889 723 388 372 275 79
54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2202 3400 1537
Size: 275 390 1198 4090
Fragments arranged by size:
4090 1198 390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2700 2787 3883 1353
Size: 1347 87 1096 3423
Fragments arranged by size:
3423 1347 1096 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2024 2248 2797 2024
Size: 224 549 5180
Fragments arranged by size:
5180 549 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2777 4016 4613 5400 5668 1239
Size: 393 632 513 1239 597 787 268 1524
Fragments arranged by size:
1524 1239 787 632 597 513 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2506 2506
Size: 5953
EcoO109I rG'GnC_Cy
Cuts at: 3110 3110
Size: 5953
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2018 2238 5100 1678
Size: 340 220 2862 2531
Fragments arranged by size:
2862 2531 340 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3009 3041 4594
Size: 32 135 32 1028 32 377 32 1553
Cuts at: 4594 4626 4668 4700 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1553 1028 377 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2619 3297 3543 669
Size: 988 281 681 678 246 3079
Fragments arranged by size:
3079 988 681 678 281 246
HindIII A'AGCT_T
Cuts at: 748 2551 748
Size: 1803 4150
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2367 2582 5067 5251 1305
Size: 1062 215 2485 184 2007
Fragments arranged by size:
2485 2007 1062 215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 2679 10
Size: 54 1425 343 760 87 3284
Fragments arranged by size:
3284 1425 760 343 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2822 3911 4270 4429 518
Size: 648 1299 357 1089 359 159 2042
Fragments arranged by size:
2042 1299 1089 648 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2900 2929 3369 4046 5194 5439 1606
Size: 565 729 29 440 677 1148 245 2120
Fragments arranged by size:
2120 1148 729 677 565 440 245 29
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 5953
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2674 513
Size: 552 1609 3792
Fragments arranged by size:
3792 1609 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 2202 3400 2202
Size: 1198 4755
NspI r_CATG'y
Cuts at: 1132 1229 3150 3311 4856 1132
Size: 97 1921 161 1545 2229
Fragments arranged by size:
2229 1921 1545 161 97
PasI CC'CwG_GG
Cuts at: 1348 2098 1348
Size: 750 5203
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2755 1108
Size: 1647 4306
PfoI T'CCnGG_A
Cuts at: 2334 2334
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3110 3110
Size: 5953
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PstI C_TGCA'G
Cuts at: 830 2868 3305 830
Size: 2038 437 3478
Fragments arranged by size:
3478 2038 437
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2900 2929 2900
Size: 29 5924
SacI G_AGCT'C
Cuts at: 2026 2250 2799 2026
Size: 224 549 5180
Fragments arranged by size:
5180 549 224
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SapI GCTCTTCn'nnn_
Cuts at: 2787 2787
Size: 5953
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 3059 4201 5913 3059
Size: 1142 1712 3099
Fragments arranged by size:
3099 1712 1142
SfcI C'TryA_G
Cuts at: 826 1045 2864 3301 4436 5117 5308 826
Size: 219 1819 437 1135 681 191 1471
Fragments arranged by size:
1819 1471 1135 681 437 219 191
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 5953
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 2213 1700
Size: 513 5440
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2107 2386 3053 4057 4958 5220
Size: 197 926 164 279 667 1004 901 262
Cuts at: 5220 820
Size: 1553
Fragments arranged by size:
1553 1004 926 901 667 279 262 197
164
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2674 3070 3392 513
Size: 552 1609 396 322 3074
Fragments arranged by size:
3074 1609 552 396 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2697 3980 3997 4150 4335 4780 325
Size: 1255 1117 1283 17 153 185 445 1498
Fragments arranged by size:
1498 1283 1255 1117 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3057 4199 5911
Size: 274 80 33 51 1443 1080 1142 1712
Cuts at: 5911 96
Size: 138
Fragments arranged by size:
1712 1443 1142 1080 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI