Restriction map for
pFN21AB7447
(Circular) (Six-base) MAPSORT of: pFN21AB7447.seq Check: 6970 from: 1 to: 5521
pFN21AB7447 5521 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 2826 3584 4968 5236 1632
Size: 1028 166 758 1384 268 1917
Fragments arranged by size:
1917 1384 1028 758 268 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 2456 1166
Size: 1290 4231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 2199 2684 3264 4836 2199
Size: 485 580 1572 2884
Fragments arranged by size:
2884 1572 580 485
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2773 2830 3056 3208 5317 1493
Size: 1280 57 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152 57
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 1870
Size: 73 903 4545
Fragments arranged by size:
4545 903 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2842 4297 618
Size: 325 217 627 1055 1455 1842
Fragments arranged by size:
1842 1455 1055 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2228 2306 2328 2840 1391
Size: 837 78 22 512 4072
Fragments arranged by size:
4072 837 512 78 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2584 928
Size: 971 685 3865
Fragments arranged by size:
3865 971 685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4132 136
Size: 107 122 71 631 605 2460 1525
Fragments arranged by size:
2460 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 3582 4738 1790
Size: 1792 1156 2573
Fragments arranged by size:
2573 1792 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2262 3252 4210
Size: 416 53 83 399 24 845 990 958
Cuts at: 4210 442
Size: 1753
Fragments arranged by size:
1753 990 958 845 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2717 4181 1239
Size: 857 621 1464 2579
Fragments arranged by size:
2579 1464 857 621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 4184 882
Size: 617 846 1839 2219
Fragments arranged by size:
2219 1839 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 2440 2602 3312 493
Size: 711 1236 162 710 2702
Fragments arranged by size:
2702 1236 711 710 162
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3328 3710 4345 275
Size: 53 83 186 936 1795 382 635 1451
Fragments arranged by size:
1795 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2370 2487 3954 4352 4626 4773 5184 2370
Size: 117 1467 398 274 147 411 2707
Fragments arranged by size:
2707 1467 411 398 274 147 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2574 2604 1178
Size: 30 165 30 1171 30 4095
Fragments arranged by size:
4095 1171 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5521
BseYI C'CCAG_C
Cuts at: 4724 5220 4724
Size: 496 5025
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2233 1163
Size: 573 497 4451
Fragments arranged by size:
4451 573 497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2971 3732 4760 1540
Size: 275 186 970 761 1028 2301
Fragments arranged by size:
2301 1028 970 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2306 2328 3582 3667 4738 2306
Size: 22 1254 85 1071 3089
Fragments arranged by size:
3089 1254 1071 85 22
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2306 2328 2840 3582 3667 4738
Size: 399 438 78 22 512 742 85 1071
Cuts at: 4738 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 512 438 399 85 78
22
BspEI T'CCGG_A
Cuts at: 2370 5184 2370
Size: 2814 2707
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2876 844
Size: 2032 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2521 4016 4190 65
Size: 1183 1273 1495 174 1396
Fragments arranged by size:
1495 1396 1273 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4165 4352 1700
Size: 110 2355 187 2869
Fragments arranged by size:
2869 2355 187 110
BsrGI T'GTAC_A
Cuts at: 96 2665 96
Size: 2569 2952
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5521
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2695 2695
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516
Size: 879 367 385 17 1452 86 12 346
Cuts at: 5516 1972
Size: 1977
Fragments arranged by size:
1977 1452 879 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4969
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2258 2682 2777 3037 3831 3851 2203
Size: 55 424 95 260 794 20 3873
Fragments arranged by size:
3873 794 424 260 95 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2006 2041 529
Size: 35 1442 35 4009
Fragments arranged by size:
4009 1442 35 35
DraI TTT'AAA
Cuts at: 2037 2825 2037
Size: 788 4733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 4528 809
Size: 507 1209 2003 1802
Fragments arranged by size:
2003 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2537 2968 3857
Size: 54 1425 50 275 18 707 431 889
Cuts at: 3857 5486 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 707 431 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 1537
Size: 275 1156 4090
Fragments arranged by size:
4090 1156 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2214 3451 1353
Size: 861 1237 3423
Fragments arranged by size:
3423 1237 861
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2304 2326 2304
Size: 22 5499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2660 2717 2826 3584 4181 4968
Size: 393 464 564 57 109 758 597 787
Cuts at: 4968 5236 1239
Size: 268 1524
Fragments arranged by size:
1524 787 758 597 564 464 393 268
109 57
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
EcoRV GAT'ATC
Cuts at: 2061 2061
Size: 5521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 5521
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 4668 1678
Size: 2990 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2574 2606 4162 4194 4236
Size: 32 135 32 1002 32 1556 32 42
Cuts at: 4236 4268 1373
Size: 32 2626
Fragments arranged by size:
2626 1556 1002 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2539 10
Size: 54 1425 343 707 2992
Fragments arranged by size:
2992 1425 707 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 3479 3838 3997 518
Size: 648 1290 1023 359 159 2042
Fragments arranged by size:
2042 1290 1023 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2495 2937 3614 4762 5007 1606
Size: 889 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 889 677 442 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4969
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NruI TCG'CGA
Cuts at: 2447 2447
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2195 2791 2873 830
Size: 1365 596 82 3478
Fragments arranged by size:
3478 1365 596 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SacI G_AGCT'C
Cuts at: 2306 2328 2306
Size: 22 5499
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2191 2387 2787 2869 4004 4685 4876
Size: 219 1146 196 400 82 1135 681 191
Cuts at: 4876 826
Size: 1471
Fragments arranged by size:
1471 1146 1135 681 400 219 196 191
82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2848 1872
Size: 976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2602 3312 493
Size: 2109 710 2702
Fragments arranged by size:
2702 2109 710
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 2418 3445 5
Size: 47 2366 1027 2081
Fragments arranged by size:
2366 2081 1027 47
StuI AGG'CCT
Cuts at: 1517 2645 1517
Size: 1128 4393
StyI C'CwwG_G
Cuts at: 513 1065 2960 513
Size: 552 1895 3074
Fragments arranged by size:
3074 1895 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2665 3767 5479
Size: 274 80 33 51 1443 688 1102 1712
Cuts at: 5479 96
Size: 138
Fragments arranged by size:
1712 1443 1102 688 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2846 1870
Size: 976 4545
XmnI GAAnn'nnTTC
Cuts at: 2417 2705 3650 2417
Size: 288 945 4288
Fragments arranged by size:
4288 945 288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI