Restriction map for
pFN21AB7445
(Circular) (Six-base) MAPSORT of: pFN21AB7445.seq Check: 2779 from: 1 to: 6199
pFN21AB7445 6199 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2523 3542 1937
Size: 586 1019 4594
Fragments arranged by size:
4594 1019 586
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 2741 2826 4262 5646 5914 1632
Size: 415 694 85 1436 1384 268 1917
Fragments arranged by size:
1917 1436 1384 694 415 268 85
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 5098 6075 1203
Size: 3895 977 1327
Fragments arranged by size:
3895 1327 977
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6199
AlwNI CAG_nnn'CTG
Cuts at: 3942 5514 3942
Size: 1572 4627
ApaI G_GGCC'C
Cuts at: 2273 2273
Size: 6199
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 2648 3508 3734 3886 5995 1493
Size: 1155 860 226 152 2109 1697
Fragments arranged by size:
2109 1697 1155 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2540 4753 160
Size: 872 1508 2213 1606
Fragments arranged by size:
2213 1606 1508 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 3524 1870
Size: 73 1581 4545
Fragments arranged by size:
4545 1581 73
BamHI G'GATC_C
Cuts at: 3529 3896 3529
Size: 367 5832
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3520 4975 618
Size: 325 217 627 1733 1455 1842
Fragments arranged by size:
1842 1733 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2273 3518 1391
Size: 882 1245 4072
Fragments arranged by size:
4072 1245 882
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2345 2741 928
Size: 971 446 396 4386
Fragments arranged by size:
4386 971 446 396
BbvCI CC'TCA_GC
Cuts at: 2013 2013
Size: 6199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4390 4424 1903
Size: 34 2453 34 3678
Fragments arranged by size:
3678 2453 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2947 4810
Size: 107 122 71 631 605 337 938 1863
Cuts at: 4810 136
Size: 1525
Fragments arranged by size:
1863 1525 938 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2197 6194 2197
Size: 3997 2202
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 2273 4260 5416 1790
Size: 483 1987 1156 2573
Fragments arranged by size:
2573 1987 1156 483
BmgBI CAC'GTC
Cuts at: 1318 2185 1318
Size: 867 5332
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3930 4888 442
Size: 416 53 83 399 24 2513 958 1753
Fragments arranged by size:
2513 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3375 1056
Size: 2319 3880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2267 2325 2424 2267
Size: 58 99 6042
Fragments arranged by size:
6042 99 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4859 1239
Size: 3620 2579
Bpu10I CC'TnA_GC
Cuts at: 2013 2264 2013
Size: 251 5948
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2766 2973 4324 5189 5487 2766
Size: 207 1351 865 298 3478
Fragments arranged by size:
3478 1351 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2139 4862 882
Size: 617 640 2723 2219
Fragments arranged by size:
2723 2219 640 617
BsaAI yAC'GTr
Cuts at: 493 1204 3990 493
Size: 711 2786 2702
Fragments arranged by size:
2786 2702 711
BsaBI GATnn'nnATC
Cuts at: 2478 6193 2478
Size: 3715 2484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4006 4388 5023 275
Size: 53 83 186 936 2473 382 635 1451
Fragments arranged by size:
2473 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4632 5030 5304 5451 5862 4632
Size: 398 274 147 411 4969
Fragments arranged by size:
4969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 1178
Size: 30 165 30 759 30 5185
Fragments arranged by size:
5185 759 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6199
BseYI C'CCAG_C
Cuts at: 5402 5898 5402
Size: 496 5703
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5626
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 4410 5438 1540
Size: 275 186 1648 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4260 4345 5416 4260
Size: 85 1071 5043
Fragments arranged by size:
5043 1071 85
BsmI GAATG_Cn'
Cuts at: 3710 3803 3710
Size: 93 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 3518 4260 4345 5416 1391
Size: 399 483 1245 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 742 483 399 85
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 4088 5818 4088
Size: 1730 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3554 844
Size: 2710 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2246 4086 1394
Size: 429 423 1840 3507
Fragments arranged by size:
3507 1840 429 423
BsrDI GCAATG_nn'
Cuts at: 65 1248 3109 3329 4694 4868 65
Size: 1183 1861 220 1365 174 1396
Fragments arranged by size:
1861 1396 1365 1183 220 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4843 5030 1700
Size: 110 3033 187 2869
Fragments arranged by size:
3033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2620 96
Size: 2524 3675
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2085 794
Size: 1291 4908
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstYI r'GATC_y
Cuts at: 1972 2197 2248 3529 3896 4281 4298 5750 5836
Size: 225 51 1281 367 385 17 1452 86
Cuts at: 5836 5848 6194 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1281 385 367 346 225 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4708
Fragments arranged by size:
4708 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3715 4509 4529 2552
Size: 1163 794 20 4222
Fragments arranged by size:
4222 1163 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 2676 3503 2676
Size: 827 5372
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2544 3269 3646
Size: 54 1425 50 275 18 714 725 377
Cuts at: 3646 4535 6164 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 725 714 377 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 2893 2931 3174 4129 1353
Size: 941 599 38 243 955 3423
Fragments arranged by size:
3423 955 941 599 243 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4786 5160 5306 387
Size: 1064 138 3197 374 146 1280
Fragments arranged by size:
3197 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2741 2826 4262 4859 5646 5914
Size: 393 415 694 85 1436 597 787 268
Cuts at: 5914 1239
Size: 1524
Fragments arranged by size:
1524 1436 787 694 597 415 393 268
85
EcoNI CCTnn'n_nnAGG
Cuts at: 2107 2107
Size: 6199
EcoO109I rG'GnC_Cy
Cuts at: 2269 2270 2269
Size: 1 6198
EcoRI G'AATT_C
Cuts at: 3508 3886 3508
Size: 378 5821
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 6199
FspI TGC'GCA
Cuts at: 4705 4705
Size: 6199
HaeII r_GCGC'y
Cuts at: 1678 2163 5346 1678
Size: 485 3183 2531
Fragments arranged by size:
3183 2531 485
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 4840 4872 4914
Size: 32 135 32 992 32 2244 32 42
Cuts at: 4914 4946 1373
Size: 32 2626
Fragments arranged by size:
2626 2244 992 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2375 3543 3789 669
Size: 988 281 437 1168 246 3079
Fragments arranged by size:
3079 1168 988 437 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5313 5497 1305
Size: 4008 184 2007
Fragments arranged by size:
4008 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2546 3271 10
Size: 54 1425 343 714 725 2938
Fragments arranged by size:
2938 1425 725 714 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 4157 4516 4675 518
Size: 648 1092 1899 359 159 2042
Fragments arranged by size:
2042 1899 1092 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3615 4292 5440 5685 1606
Size: 2009 677 1148 245 2120
Fragments arranged by size:
2120 2009 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4708
Fragments arranged by size:
4708 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 3371 1052
Size: 2319 3880
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 3557 5102 1132
Size: 97 2328 1545 2229
Fragments arranged by size:
2328 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6199
PfoI T'CCnGG_A
Cuts at: 3387 3387
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 2269 2269
Size: 6199
PstI C_TGCA'G
Cuts at: 830 2111 3551 830
Size: 1281 1440 3478
Fragments arranged by size:
3478 1440 1281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SapI GCTCTTCn'nnn_
Cuts at: 2931 2931
Size: 6199
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 4447 6159 4447
Size: 1712 4487
SfcI C'TryA_G
Cuts at: 826 1045 2107 3547 4682 5363 5554 826
Size: 219 1062 1440 1135 681 191 1471
Fragments arranged by size:
1471 1440 1135 1062 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2781 2988 4303 5204 5466 820
Size: 197 926 838 207 1315 901 262 1553
Fragments arranged by size:
1553 1315 926 901 838 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 2767 4123 5
Size: 47 2715 1356 2081
Fragments arranged by size:
2715 2081 1356 47
StuI AGG'CCT
Cuts at: 1517 3193 1517
Size: 1676 4523
StyI C'CwwG_G
Cuts at: 513 1065 2004 2174 3638 513
Size: 552 939 170 1464 3074
Fragments arranged by size:
3074 1464 939 552 170
SwaI ATTT'AAAT
Cuts at: 2676 2676
Size: 6199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 4226 4243 4396 4581 5026 325
Size: 1255 543 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 543 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 3487 4445
Size: 274 80 33 51 1443 643 867 958
Cuts at: 4445 6157 96
Size: 1712 138
Fragments arranged by size:
1712 1443 958 867 643 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 2370 1316
Size: 1054 5145
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 4328 4328
Size: 6199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI XcmI