Restriction map for
pFN21AB7442
(Circular) (Six-base) MAPSORT of: pFN21AB7442.seq Check: 8740 from: 1 to: 5740
pFN21AB7442 5740 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2536 3550 278
Size: 53 83 186 936 1000 1014 2468
Fragments arranged by size:
2468 1014 1000 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 3041 3803 5187 5455 1632
Size: 1158 251 762 1384 268 1917
Fragments arranged by size:
1917 1384 1158 762 268 251
AflII C'TTAA_G
Cuts at: 820 1017 2722 820
Size: 197 1705 3838
Fragments arranged by size:
3838 1705 197
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 2532 4469 2532
Size: 1937 3803
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 2813 3483 5055 2813
Size: 670 1572 3498
Fragments arranged by size:
3498 1572 670
ApaLI G'TGCA_C
Cuts at: 2264 3797 4953 2264
Size: 1533 1156 3051
Fragments arranged by size:
3051 1533 1156
ApoI r'AATT_y
Cuts at: 1493 3026 3049 3275 3427 5536 1493
Size: 1533 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1533 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2444 2903 3059 1391
Size: 1053 459 156 4072
Fragments arranged by size:
4072 1053 459 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2939 928
Size: 971 1040 3729
Fragments arranged by size:
3729 1040 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3931 3965 1903
Size: 34 50 34 1910 34 3678
Fragments arranged by size:
3678 1910 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4351 136
Size: 107 122 71 631 605 2679 1525
Fragments arranged by size:
2679 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 2268 3801 4957 1790
Size: 478 1533 1156 2573
Fragments arranged by size:
2573 1533 1156 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2278 3471 4429
Size: 416 53 83 399 24 861 1193 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1193 958 861 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3033 3033
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2399 4400 1239
Size: 880 280 2001 2579
Fragments arranged by size:
2579 2001 880 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2850 4403 882
Size: 617 1351 1553 2219
Fragments arranged by size:
2219 1553 1351 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 2594 5734 2594
Size: 3140 2600
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2533 3547 3929 4564
Size: 53 83 186 936 1000 1014 382 635
Cuts at: 4564 275
Size: 1451
Fragments arranged by size:
1451 1014 1000 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2655 1186
Size: 1000 469 4271
Fragments arranged by size:
4271 1000 469
BseYI C'CCAG_C
Cuts at: 2126 2354 2562 2659 2813 4943 5439 2126
Size: 228 208 97 154 2130 496 2427
Fragments arranged by size:
2427 2130 496 228 208 154 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2268 3801 3886 4957 2208
Size: 60 1533 85 1071 2991
Fragments arranged by size:
2991 1533 1071 85 60
BsmI GAATG_Cn'
Cuts at: 2456 3251 3344 2456
Size: 795 93 4852
Fragments arranged by size:
4852 795 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 2540
Size: 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2268 2444 2903 3059 3801 3886
Size: 399 418 60 176 459 156 742 85
Cuts at: 3886 4957 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 459 418 399 176 156
85 60
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3095 844
Size: 2251 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2248 4235 4409 65
Size: 1183 1000 1987 174 1396
Fragments arranged by size:
1987 1396 1183 1000 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2295 96
Size: 2199 3541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2551 2362
Size: 189 5551
BstYI r'GATC_y
Cuts at: 1972 2190 2627 3070 3437 3822 3839 5291 5377
Size: 218 437 443 367 385 17 1452 86
Cuts at: 5377 5389 5735 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 443 437 385 367 346 218
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 2966 3256 4050 4070 2966
Size: 290 794 20 4636
Fragments arranged by size:
4636 794 290 20
ClaI AT'CG_AT
Cuts at: 1554 2482 1554
Size: 928 4812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 2031 3044 2031
Size: 1013 4727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 3187 4076
Size: 54 1425 50 275 18 534 823 889
Cuts at: 4076 5705 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 823 534 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2673 2759 3670 1353
Size: 860 460 86 911 3423
Fragments arranged by size:
3423 911 860 460 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 4701 4847 387
Size: 1064 138 2738 374 146 1280
Fragments arranged by size:
2738 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2399 2790 3041 3803 4400 5187
Size: 393 487 280 391 251 762 597 787
Cuts at: 5187 5455 1239
Size: 268 1524
Fragments arranged by size:
1524 787 762 597 487 393 391 280
268 251
EcoO109I rG'GnC_Cy
Cuts at: 2693 2766 2800 2693
Size: 73 34 5633
Fragments arranged by size:
5633 73 34
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2844 2844
Size: 5740
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 4887 1678
Size: 3209 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2035 2067 2518 2550 2926
Size: 32 135 32 463 32 451 32 376
Cuts at: 2926 2958 2974 3006 4381 4413 4455 4487 1373
Size: 32 16 32 1375 32 42 32 2626
Fragments arranged by size:
2626 1375 463 451 376 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2602 3084 3330 669
Size: 988 281 664 482 246 3079
Fragments arranged by size:
3079 988 664 482 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 2557 3339 2557
Size: 782 4958
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2540 4854 5038 1305
Size: 1235 2314 184 2007
Fragments arranged by size:
2314 2007 1235 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 10
Size: 54 1425 343 534 3384
Fragments arranged by size:
3384 1425 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3698 4057 4216 518
Size: 648 2532 359 159 2042
Fragments arranged by size:
2532 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2813 3156 3833 4981 5226 1606
Size: 1207 343 677 1148 245 2120
Fragments arranged by size:
2120 1207 1148 677 343 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 2362 3098 4643 1132
Size: 97 1133 736 1545 2229
Fragments arranged by size:
2229 1545 1133 736 97
PasI CC'CwG_GG
Cuts at: 1348 2178 1348
Size: 830 4910
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2766 2800 2766
Size: 34 5706
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2813 2813
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SanDI GG'GwC_CC
Cuts at: 2800 2800
Size: 5740
SapI GCTCTTCn'nnn_
Cuts at: 2213 2213
Size: 5740
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 2883 3988 5700 2883
Size: 1105 1712 2923
Fragments arranged by size:
2923 1712 1105
SfcI C'TryA_G
Cuts at: 826 1045 2445 3088 4223 4904 5095 826
Size: 219 1400 643 1135 681 191 1471
Fragments arranged by size:
1471 1400 1135 681 643 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2722 3844 4745 5007 820
Size: 197 926 779 1122 901 262 1553
Fragments arranged by size:
1553 1122 926 901 779 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 2362 3098 1132
Size: 1230 736 3774
Fragments arranged by size:
3774 1230 736
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2436 3179 513
Size: 552 1371 743 3074
Fragments arranged by size:
3074 1371 743 552
SwaI ATTT'AAAT
Cuts at: 2031 2031
Size: 5740
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 2881 3986
Size: 274 80 33 51 1443 318 586 1105
Cuts at: 3986 5698 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1105 586 318 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 2114 3869 2114
Size: 1755 3985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2534 3548 276
Size: 53 83 186 936 1000 1014 2468
Fragments arranged by size:
2468 1014 1000 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SexAI SfiI SfoI SrfI XcmI