Restriction map for
pFN21AB7441
(Circular) (Six-base) MAPSORT of: pFN21AB7441.seq Check: 5530 from: 1 to: 5350
pFN21AB7441 5350 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2604 3160 278
Size: 53 83 186 936 1068 556 2468
Fragments arranged by size:
2468 1068 936 556 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2178 2693 1937
Size: 241 515 4594
Fragments arranged by size:
4594 515 241
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3413 4797 5065 1632
Size: 1781 1384 268 1917
Fragments arranged by size:
1917 1781 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5153
AflIII A'CryG_T
Cuts at: 1203 2059 4249 5226 1203
Size: 856 2190 977 1327
Fragments arranged by size:
2190 1327 977 856
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 2060 1166
Size: 894 4456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2052 1184
Size: 868 4482
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 2369
Size: 32 5318
AlwNI CAG_nnn'CTG
Cuts at: 3093 4665 3093
Size: 1572 3778
ApaLI G'TGCA_C
Cuts at: 2008 2063 3407 4563 2008
Size: 55 1344 1156 2795
Fragments arranged by size:
2795 1344 1156 55
ApoI r'AATT_y
Cuts at: 1493 2659 2885 3037 5146 1493
Size: 1166 226 152 2109 1697
Fragments arranged by size:
2109 1697 1166 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2619 3904 160
Size: 872 1587 1285 1606
Fragments arranged by size:
1606 1587 1285 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 1870
Size: 73 732 4545
Fragments arranged by size:
4545 732 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 4126 618
Size: 325 217 627 884 1455 1842
Fragments arranged by size:
1842 1455 884 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2105 2598 2669 1391
Size: 714 493 71 4072
Fragments arranged by size:
4072 714 493 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3541 3575 1903
Size: 34 1604 34 3678
Fragments arranged by size:
3678 1604 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 4452 3242
Size: 1210 4140
BclI T'GATC_A
Cuts at: 1548 2551 1548
Size: 1003 4347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3961 136
Size: 107 122 71 631 605 2289 1525
Fragments arranged by size:
2289 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2033 5345 2033
Size: 3312 2038
BlpI GC'TnA_GC
Cuts at: 2767 2767
Size: 5350
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2067 3411 4567 1790
Size: 222 55 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 222 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3081 4039 442
Size: 416 53 83 399 24 1664 958 1753
Fragments arranged by size:
1753 1664 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 4010 1239
Size: 1340 1431 2579
Fragments arranged by size:
2579 1431 1340
Bpu10I CC'TnA_GC
Cuts at: 2268 2268
Size: 5350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 3475 4340 4638 2440
Size: 1035 865 298 3152
Fragments arranged by size:
3152 1035 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2195 4013 882
Size: 617 696 1818 2219
Fragments arranged by size:
2219 1818 696 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3141 493
Size: 711 1170 767 2702
Fragments arranged by size:
2702 1170 767 711
BsaBI GATnn'nnATC
Cuts at: 5344 5344
Size: 5350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 3157 3539 4174
Size: 53 83 186 936 1068 556 382 635
Cuts at: 4174 275
Size: 1451
Fragments arranged by size:
1451 1068 936 635 556 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3783 4181 4455 4602 5013 3783
Size: 398 274 147 411 4120
Fragments arranged by size:
4120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5125
Fragments arranged by size:
5125 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5350
BseYI C'CCAG_C
Cuts at: 4553 5049 4553
Size: 496 4854
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4777
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3561 4589 1540
Size: 275 186 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2067 2174 2598 3411 3496 4567 2012
Size: 55 107 424 813 85 1071 2795
Fragments arranged by size:
2795 1071 813 424 107 85 55
BsmI GAATG_Cn'
Cuts at: 2861 2954 2861
Size: 93 5257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2067 2105 2174 2598 2669 3411
Size: 399 222 55 38 69 424 71 742
Cuts at: 3411 3496 4567 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 222 85 71
69 55 38
BspEI T'CCGG_A
Cuts at: 5013 5013
Size: 5350
BspHI T'CATG_A
Cuts at: 2629 3239 4969 2629
Size: 610 1730 3010
Fragments arranged by size:
3010 1730 610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 844
Size: 1861 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3237 1394
Size: 429 1414 3507
Fragments arranged by size:
3507 1414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 2483 3845 4019 65
Size: 1183 1188 47 1362 174 1396
Fragments arranged by size:
1396 1362 1188 1183 174 47
BsrFI r'CCGG_y
Cuts at: 1700 1810 3994 4181 1700
Size: 110 2184 187 2869
Fragments arranged by size:
2869 2184 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 3410 4422 3410
Size: 1012 4338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5350
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstXI CCAn_nnnn'nTGG
Cuts at: 2097 2097
Size: 5350
BstYI r'GATC_y
Cuts at: 1972 2033 2680 3047 3432 3449 4901 4987 4999
Size: 61 647 367 385 17 1452 86 12
Cuts at: 4999 5345 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 647 385 367 346 86 61
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2111 2185 513
Size: 552 1046 74 3678
Fragments arranged by size:
3678 1046 552 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4611
Fragments arranged by size:
4611 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 2501 2866 3660 3680 2500
Size: 1 365 794 20 4170
Fragments arranged by size:
4170 794 365 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2654 2654
Size: 5350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2797 3686 5315
Size: 54 1425 50 275 18 967 889 1629
Cuts at: 5315 8
Size: 43
Fragments arranged by size:
1629 1425 967 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 1537
Size: 275 985 4090
Fragments arranged by size:
4090 985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 3280 1353
Size: 873 1054 3423
Fragments arranged by size:
3423 1054 873
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3937 4311 4457 387
Size: 1064 138 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2596
Size: 5350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 3413 4010 4797 5065 1239
Size: 393 947 834 597 787 268 1524
Fragments arranged by size:
1524 947 834 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 5350
FspI TGC'GCA
Cuts at: 3856 3856
Size: 5350
HaeII r_GCGC'y
Cuts at: 1678 4497 1678
Size: 2819 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 3991 4023 4065
Size: 32 135 32 910 32 1477 32 42
Cuts at: 4065 4097 1373
Size: 32 2626
Fragments arranged by size:
2626 1477 910 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2694 2940 669
Size: 988 281 756 246 3079
Fragments arranged by size:
3079 988 756 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5350
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2949 2949
Size: 5350
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4464 4648 1305
Size: 3159 184 2007
Fragments arranged by size:
3159 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3528
Fragments arranged by size:
3528 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2076 2082 2216 3308 3667 3826
Size: 648 894 16 6 134 1092 359 159
Cuts at: 3826 518
Size: 2042
Fragments arranged by size:
2042 1092 894 648 359 159 134 16
6
MspA1I CmG'CkG
Cuts at: 1606 2557 2648 2766 3443 4591 4836 1606
Size: 951 91 118 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 245 118 91
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4798
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2797 2797
Size: 5350
NspI r_CATG'y
Cuts at: 1132 1229 2063 2221 2461 2708 4253 1132
Size: 97 834 158 240 247 1545 2229
Fragments arranged by size:
2229 1545 834 247 240 158 97
PasI CC'CwG_GG
Cuts at: 1348 2587 1348
Size: 1239 4111
PciI A'CATG_T
Cuts at: 2059 4249 2059
Size: 2190 3160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5350
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 4932 4964 3419
Size: 32 1481 32 3805
Fragments arranged by size:
3805 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5350
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PstI C_TGCA'G
Cuts at: 830 2319 2507 2702 830
Size: 1489 188 195 3478
Fragments arranged by size:
3478 1489 195 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
PvuII CAG'CTG
Cuts at: 2557 2648 2557
Size: 91 5259
SacI G_AGCT'C
Cuts at: 2598 2598
Size: 5350
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SfcI C'TryA_G
Cuts at: 826 1045 2315 2503 2698 3833 4514 4705 826
Size: 219 1270 188 195 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 219 195 191 188
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2677 1872
Size: 805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 3454 4355 4617 820
Size: 197 926 476 1035 901 262 1553
Fragments arranged by size:
1553 1035 926 901 476 262 197
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5350
StyI C'CwwG_G
Cuts at: 513 1065 2789 513
Size: 552 1724 3074
Fragments arranged by size:
3074 1724 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3377 3394 3547 3732 4177 325
Size: 1255 1797 17 153 185 445 1498
Fragments arranged by size:
1797 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3596 5308 96
Size: 274 80 33 51 1443 1619 1712 138
Fragments arranged by size:
1712 1619 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5310 5342 5310
Size: 32 5318
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5350
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2584 2359
Size: 225 5125
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5350
XmaI C'CCGG_G
Cuts at: 1870 2675 1870
Size: 805 4545
XmnI GAAnn'nnTTC
Cuts at: 3479 3479
Size: 5350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2602 3158 276
Size: 53 83 186 936 1068 556 2468
Fragments arranged by size:
2468 1068 936 556 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI