Restriction map for
pFN21AB7440
(Circular) (Six-base) MAPSORT of: pFN21AB7440.seq Check: 3434 from: 1 to: 5161
pFN21AB7440 5161 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2971 278
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2504 1937
Size: 567 4594
Acc65I G'GTAC_C
Cuts at: 2482 2482
Size: 5161
AclI AA'CG_TT
Cuts at: 1563 3288 3661 1563
Size: 1725 373 3063
Fragments arranged by size:
3063 1725 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 3224 4608 4876 1632
Size: 704 888 1384 268 1917
Fragments arranged by size:
1917 1384 888 704 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4964
AflIII A'CryG_T
Cuts at: 1203 2373 4060 5037 1203
Size: 1170 1687 977 1327
Fragments arranged by size:
1687 1327 1170 977
AgeI A'CCGG_T
Cuts at: 3992 3992
Size: 5161
AhdI GACnn_n'nnGTC
Cuts at: 3890 3890
Size: 5161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2421 1184
Size: 1237 3924
AlwNI CAG_nnn'CTG
Cuts at: 2904 4476 2904
Size: 1572 3589
ApaLI G'TGCA_C
Cuts at: 3218 4374 3218
Size: 1156 4005
ApoI r'AATT_y
Cuts at: 1493 2470 2696 2848 4957 1493
Size: 977 226 152 2109 1697
Fragments arranged by size:
2109 1697 977 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3715 160
Size: 872 2683 1606
Fragments arranged by size:
2683 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2486 1870
Size: 73 208 335 4545
Fragments arranged by size:
4545 335 208 73
BamHI G'GATC_C
Cuts at: 2491 2858 2491
Size: 367 4794
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2482 3937 618
Size: 325 217 627 360 335 1455 1842
Fragments arranged by size:
1842 1455 627 360 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2394 2480 1391
Size: 1003 86 4072
Fragments arranged by size:
4072 1003 86
BbeI G_GCGC'C
Cuts at: 2151 2151
Size: 5161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3352 3386 1903
Size: 34 1415 34 3678
Fragments arranged by size:
3678 1415 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4263 3053
Size: 1210 3951
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3772 136
Size: 107 122 71 631 605 2100 1525
Fragments arranged by size:
2100 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5156 5156
Size: 5161
BlpI GC'TnA_GC
Cuts at: 2578 2578
Size: 5161
Bme1580I G_kGCm'C
Cuts at: 1790 2446 3222 4378 1790
Size: 656 776 1156 2573
Fragments arranged by size:
2573 1156 776 656
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2892 3850 442
Size: 416 53 83 399 24 1475 958 1753
Fragments arranged by size:
1753 1475 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 3821 1239
Size: 1031 1551 2579
Fragments arranged by size:
2579 1551 1031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 3286 4151 4449 2069
Size: 1217 865 298 2781
Fragments arranged by size:
2781 1217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3824 882
Size: 617 2325 2219
Fragments arranged by size:
2325 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2447 2952 493
Size: 711 1243 505 2702
Fragments arranged by size:
2702 1243 711 505
BsaBI GATnn'nnATC
Cuts at: 5155 5155
Size: 5161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2968 3350 3985
Size: 53 83 186 936 615 820 382 635
Cuts at: 3985 275
Size: 1451
Fragments arranged by size:
1451 936 820 635 615 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3594 3992 4266 4413 4824 3594
Size: 398 274 147 411 3931
Fragments arranged by size:
3931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2279 2309 1178
Size: 30 165 30 876 30 4030
Fragments arranged by size:
4030 876 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5161
BseYI C'CCAG_C
Cuts at: 2156 2173 4364 4860 2156
Size: 17 2191 496 2457
Fragments arranged by size:
2457 2191 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4588
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2611 3372 4400 1540
Size: 275 186 610 761 1028 2301
Fragments arranged by size:
2301 1028 761 610 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 3222 3307 4378 2394
Size: 828 85 1071 3177
Fragments arranged by size:
3177 1071 828 85
BsiWI C'GTAC_G
Cuts at: 2073 2073
Size: 5161
BsmI GAATG_Cn'
Cuts at: 2672 2765 2672
Size: 93 5068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2446 2480 3222 3307 4378 1391
Size: 399 604 52 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 85 52 34
BspEI T'CCGG_A
Cuts at: 4824 4824
Size: 5161
BspHI T'CATG_A
Cuts at: 3050 4780 3050
Size: 1730 3431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2516 844
Size: 1672 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 1394
Size: 429 1225 3507
Fragments arranged by size:
3507 1225 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3656 3830 65
Size: 1183 2408 174 1396
Fragments arranged by size:
2408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2144 3805 3992 1700
Size: 110 334 1661 187 2869
Fragments arranged by size:
2869 1661 334 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5161
BssSI C'ACGA_G
Cuts at: 3221 4233 3221
Size: 1012 4149
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5161
BstBI TT'CG_AA
Cuts at: 3965 3965
Size: 5161
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5161
BstXI CCAn_nnnn'nTGG
Cuts at: 2439 2439
Size: 5161
BstYI r'GATC_y
Cuts at: 1972 2491 2858 3243 3260 4712 4798 4810 5156
Size: 519 367 385 17 1452 86 12 346
Cuts at: 5156 1972
Size: 1977
Fragments arranged by size:
1977 1452 519 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 513
Size: 552 939 198 3472
Fragments arranged by size:
3472 939 552 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4422
Fragments arranged by size:
4422 683 56
BtsI GCAGTG_nn'
Cuts at: 2677 3471 3491 2677
Size: 794 20 4347
Fragments arranged by size:
4347 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5126
DraI TTT'AAA
Cuts at: 2465 2465
Size: 5161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4168 809
Size: 507 2852 1802
Fragments arranged by size:
2852 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2325 2608
Size: 54 1425 50 275 18 312 183 283
Cuts at: 2608 3497 5126 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 312 283 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2608 1537
Size: 275 796 4090
Fragments arranged by size:
4090 796 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 3091 1353
Size: 1029 709 3423
Fragments arranged by size:
3423 1029 709
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2070 2105 2270 3748 4122 4268
Size: 1064 138 481 35 165 1478 374 146
Cuts at: 4268 387
Size: 1280
Fragments arranged by size:
1478 1280 1064 481 374 165 146 138
35
EcoICRI GAG'CTC
Cuts at: 2392 2392
Size: 5161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2336 3224 3821 4608 4876 1239
Size: 393 638 66 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 638 597 393 268 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2083 2083
Size: 5161
EcoO109I rG'GnC_Cy
Cuts at: 2245 2245
Size: 5161
EcoRI G'AATT_C
Cuts at: 2470 2848 2470
Size: 378 4783
FspI TGC'GCA
Cuts at: 3667 3667
Size: 5161
HaeII r_GCGC'y
Cuts at: 1678 2151 2217 4308 1678
Size: 473 66 2091 2531
Fragments arranged by size:
2531 2091 473 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3802 3834 3876 3908 1373
Size: 32 135 32 2230 32 42 32 2626
Fragments arranged by size:
2626 2230 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2070 2505 2751 669
Size: 988 281 132 435 246 3079
Fragments arranged by size:
3079 988 435 281 246 132
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5161
HpaI GTT'AAC
Cuts at: 2751 2751
Size: 5161
KasI G'GCGC_C
Cuts at: 2147 2147
Size: 5161
KpnI G_GTAC'C
Cuts at: 2486 2486
Size: 5161
MfeI C'AATT_G
Cuts at: 2760 2760
Size: 5161
MluI A'CGCG_T
Cuts at: 2373 5037 2373
Size: 2664 2497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4275 4459 1305
Size: 2970 184 2007
Fragments arranged by size:
2970 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3339
Fragments arranged by size:
3339 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2403 3119 3478 3637 518
Size: 648 1237 716 359 159 2042
Fragments arranged by size:
2042 1237 716 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2108 2190 2242 2347 2577 3254 4402
Size: 425 77 82 52 105 230 677 1148
Cuts at: 4402 4647 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 425 245 230 105 82
77 52
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 5161
NarI GG'CG_CC
Cuts at: 2148 2148
Size: 5161
NcoI C'CATG_G
Cuts at: 513 1065 2004 2202 513
Size: 552 939 198 3472
Fragments arranged by size:
3472 939 552 198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5161
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 5161
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5161
NotI GC'GGCC_GC
Cuts at: 2608 2608
Size: 5161
NspI r_CATG'y
Cuts at: 1132 1229 2264 2519 4064 1132
Size: 97 1035 255 1545 2229
Fragments arranged by size:
2229 1545 1035 255 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5161
PciI A'CATG_T
Cuts at: 4060 4060
Size: 5161
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5161
PmeI GTTT'AAAC
Cuts at: 2465 2465
Size: 5161
PmlI CAC'GTG
Cuts at: 2447 2447
Size: 5161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 4743 4775 3230
Size: 32 1481 32 3616
Fragments arranged by size:
3616 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2245
Size: 5161
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5161
PsiI TTA'TAA
Cuts at: 2731 2731
Size: 5161
PstI C_TGCA'G
Cuts at: 830 2241 2513 830
Size: 1411 272 3478
Fragments arranged by size:
3478 1411 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5161
PvuII CAG'CTG
Cuts at: 2190 2190
Size: 5161
SacI G_AGCT'C
Cuts at: 2394 2394
Size: 5161
SalI G'TCGA_C
Cuts at: 1936 2503 1936
Size: 567 4594
SapI GCTCTTCn'nnn_
Cuts at: 2382 2382
Size: 5161
SbfI CC_TGCA'GG
Cuts at: 2513 2513
Size: 5161
ScaI AGT'ACT
Cuts at: 2062 3409 5121 2062
Size: 1347 1712 2102
Fragments arranged by size:
2102 1712 1347
SfcI C'TryA_G
Cuts at: 826 1045 2237 2299 2509 3644 4325 4516 826
Size: 219 1192 62 210 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 681 219 210 191 62
SfoI GGC'GCC
Cuts at: 2149 2149
Size: 5161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5161
SmaI CCC'GGG
Cuts at: 1872 2488 1872
Size: 616 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 3265 4166 4428 820
Size: 197 926 105 1217 901 262 1553
Fragments arranged by size:
1553 1217 926 901 262 197 105
SnaBI TAC'GTA
Cuts at: 493 2952 493
Size: 2459 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5161
SphI G_CATG'C
Cuts at: 1132 2519 1132
Size: 1387 3774
SspI AAT'ATT
Cuts at: 5 52 3085 5
Size: 47 3033 2081
Fragments arranged by size:
3033 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5161
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 2600 513
Size: 552 939 198 398 3074
Fragments arranged by size:
3074 939 552 398 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3188 3205 3358 3543 3988 325
Size: 1255 1608 17 153 185 445 1498
Fragments arranged by size:
1608 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2060 3407 5119
Size: 274 80 33 51 1443 83 1347 1712
Cuts at: 5119 96
Size: 138
Fragments arranged by size:
1712 1443 1347 274 138 83 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5121 5153 5121
Size: 32 5129
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5161
XbaI T'CTAG_A
Cuts at: 2497 2497
Size: 5161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5161
XmaI C'CCGG_G
Cuts at: 1870 2486 1870
Size: 616 4545
XmnI GAAnn'nnTTC
Cuts at: 3290 3290
Size: 5161
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2969 276
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI