Restriction map for
pFN21AB7433
(Circular) (Six-base) MAPSORT of: pFN21AB7433.seq Check: 3090 from: 1 to: 5662
pFN21AB7433 5662 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 2927 3725 5109 5377 1632
Size: 1086 209 798 1384 268 1917
Fragments arranged by size:
1917 1384 1086 798 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 2658 4561 5538 1203
Size: 1455 1903 977 1327
Fragments arranged by size:
1903 1455 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 3405 4977 3405
Size: 1572 4090
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2254 2971 3197 3349 5458 1493
Size: 761 717 226 152 2109 1697
Fragments arranged by size:
2109 1697 761 717 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 2983 4438 618
Size: 325 217 627 975 221 1455 1842
Fragments arranged by size:
1842 1455 975 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2693 2981 1391
Size: 1302 288 4072
Fragments arranged by size:
4072 1302 288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2710 2824 928
Size: 971 811 114 3766
Fragments arranged by size:
3766 971 811 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2152 3554 4764 2152
Size: 1402 1210 3050
Fragments arranged by size:
3050 1402 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BfrBI ATG'CAT
Cuts at: 2291 2291
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 2692 3079 2692
Size: 387 5275
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4879 1790
Size: 1933 1156 2573
Fragments arranged by size:
2573 1933 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 3393 4351
Size: 416 53 83 399 24 898 1078 958
Cuts at: 4351 442
Size: 1753
Fragments arranged by size:
1753 1078 958 898 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 4322 1239
Size: 1161 1922 2579
Fragments arranged by size:
2579 1922 1161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2779 3787 4652 4950 2779
Size: 1008 865 298 3491
Fragments arranged by size:
3491 1008 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2546 1186
Size: 1360 4302
BseYI C'CCAG_C
Cuts at: 4865 5361 4865
Size: 496 5166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5089
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2693 3723 3808 4879 2693
Size: 1030 85 1071 3476
Fragments arranged by size:
3476 1071 1030 85
BsmI GAATG_Cn'
Cuts at: 2427 3173 3266 2427
Size: 746 93 4823
Fragments arranged by size:
4823 746 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2693 2981 3723 3808 4879 1391
Size: 399 903 288 742 85 1071 2174
Fragments arranged by size:
2174 1071 903 742 399 288 85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 844
Size: 2173 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2156 3549 1394
Size: 429 333 1393 3507
Fragments arranged by size:
3507 1393 429 333
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 2262 96
Size: 2166 3496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657
Size: 1020 367 385 17 1452 86 12 346
Cuts at: 5657 1972
Size: 1977
Fragments arranged by size:
1977 1452 1020 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2558 2681 2558
Size: 123 5539
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3820
Fragments arranged by size:
3820 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3109 3998
Size: 54 1425 50 275 18 546 733 889
Cuts at: 3998 5627 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 733 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2230 3592 1353
Size: 764 113 1362 3423
Fragments arranged by size:
3423 1362 764 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2486 4249 4623 4769 387
Size: 1064 138 897 1763 374 146 1280
Fragments arranged by size:
1763 1280 1064 897 374 146 138
EcoICRI GAG'CTC
Cuts at: 2691 2691
Size: 5662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2718 2927 3725 4322 5109 5377
Size: 393 768 318 209 798 597 787 268
Cuts at: 5377 1239
Size: 1524
Fragments arranged by size:
1524 798 787 768 597 393 318 268
209
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2860 2892 4303 4335 4377
Size: 32 135 32 1288 32 1411 32 42
Cuts at: 4377 4409 1373
Size: 32 2626
Fragments arranged by size:
2626 1411 1288 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 3006 3252 669
Size: 988 281 589 479 246 3079
Fragments arranged by size:
3079 988 589 479 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 2017 3261 2017
Size: 1244 4418
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 4776 4960 1305
Size: 1236 2235 184 2007
Fragments arranged by size:
2235 2007 1236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3294
Fragments arranged by size:
3294 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2324 3620 3979 4138 518
Size: 648 1011 147 1296 359 159 2042
Fragments arranged by size:
2042 1296 1011 648 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2696 3078 3755 4903 5148 1606
Size: 1090 382 677 1148 245 2120
Fragments arranged by size:
2120 1148 1090 677 382 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3820
Fragments arranged by size:
3820 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NsiI A_TGCA'T
Cuts at: 2293 2293
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2295 2551 2662 2740 3020 4565 1132
Size: 97 1066 256 111 78 280 1545 2229
Fragments arranged by size:
2229 1545 1066 280 256 111 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 2658 4561 2658
Size: 1903 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PfoI T'CCnGG_A
Cuts at: 2416 2416
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2696 2696
Size: 5662
SacI G_AGCT'C
Cuts at: 2693 2693
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2455 2725 3010 4145 4826 5017 826
Size: 219 1410 270 285 1135 681 191 1471
Fragments arranged by size:
1471 1410 1135 681 285 270 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2794 3766 4667 4929 820
Size: 197 926 851 972 901 262 1553
Fragments arranged by size:
1553 972 926 901 851 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 3101 513
Size: 552 939 351 746 3074
Fragments arranged by size:
3074 939 746 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 2254 2382 3689 3706 3859 4044
Size: 1255 459 215 128 1307 17 153 185
Cuts at: 4044 4489 325
Size: 445 1498
Fragments arranged by size:
1498 1307 1255 459 445 215 185 153
128 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2262 2778
Size: 274 80 33 51 1443 204 81 516
Cuts at: 2778 3908 5620 96
Size: 1130 1712 138
Fragments arranged by size:
1712 1443 1130 516 274 204 138 81
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI