(Circular) (Six-base) MAPSORT of: pFN21AB7431.seq Check: 1588 from: 1 to: 5629 pFN21AB7431 5629 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2906 3439 278 Size: 53 83 186 936 1370 533 2468 Fragments arranged by size: 2468 1370 936 533 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2107 2907 3692 5076 5344 1632 Size: 475 800 785 1384 268 1917 Fragments arranged by size: 1917 1384 800 785 475 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2873 1184 Size: 1689 3940 AlwNI CAG_nnn'CTG Cuts at: 2324 3372 4944 2324 Size: 1048 1572 3009 Fragments arranged by size: 3009 1572 1048 ApaI G_GGCC'C Cuts at: 2310 2310 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2114 2450 2950 4405 Size: 325 217 627 248 79 336 500 1455 Cuts at: 4405 618 Size: 1842 Fragments arranged by size: 1842 1455 627 500 336 325 248 217 79 BanII G_rGCy'C Cuts at: 1391 2021 2310 2948 1391 Size: 630 289 638 4072 Fragments arranged by size: 4072 638 630 289 BbeI G_GCGC'C Cuts at: 2454 2454 Size: 5629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 928 Size: 971 645 4013 Fragments arranged by size: 4013 971 645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3820 3854 1903 Size: 34 50 34 1799 34 3678 Fragments arranged by size: 3678 1799 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2727 3521 4731 2727 Size: 794 1210 3625 Fragments arranged by size: 3625 1210 794 BclI T'GATC_A Cuts at: 1548 2520 1548 Size: 972 4657 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2016 2044 2279 Size: 107 122 71 631 605 344 28 235 Cuts at: 2279 2387 4240 136 Size: 108 1853 1525 Fragments arranged by size: 1853 1525 631 605 344 235 122 108 107 71 28 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 2546 3046 2546 Size: 500 5129 Bme1580I G_kGCm'C Cuts at: 1790 2117 2284 2310 3690 4846 1790 Size: 327 167 26 1380 1156 2573 Fragments arranged by size: 2573 1380 1156 327 167 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2911 3360 4318 Size: 416 53 83 399 24 1494 449 958 Cuts at: 4318 442 Size: 1753 Fragments arranged by size: 1753 1494 958 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2381 2585 4289 1239 Size: 1112 30 204 1704 2579 Fragments arranged by size: 2579 1704 1112 204 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2451 2903 3436 3818 Size: 53 83 186 936 918 452 533 382 Cuts at: 3818 4453 275 Size: 635 1451 Fragments arranged by size: 1451 936 918 635 533 452 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2719 4062 4460 4734 4881 5292 2719 Size: 1343 398 274 147 411 3056 Fragments arranged by size: 3056 1343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2255 2258 2282 2348 1186 Size: 1015 54 3 24 66 4467 Fragments arranged by size: 4467 1015 66 54 24 3 BseYI C'CCAG_C Cuts at: 2274 4832 5328 2274 Size: 2558 496 2575 Fragments arranged by size: 2575 2558 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2310 1163 Size: 573 423 151 4482 Fragments arranged by size: 4482 573 423 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 3079 3840 4868 1540 Size: 275 186 153 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 3690 3775 4846 3690 Size: 85 1071 4473 Fragments arranged by size: 4473 1071 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 BsmBI CGTCTCn'nnnn_ Cuts at: 2894 2894 Size: 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2117 2284 2310 2948 3690 3775 Size: 399 231 96 167 26 638 742 85 Cuts at: 3775 4846 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 638 399 231 167 96 85 26 BspEI T'CCGG_A Cuts at: 2719 5292 2719 Size: 2573 3056 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2260 2419 3516 1394 Size: 429 437 159 1097 3507 Fragments arranged by size: 3507 1097 437 429 159 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2365 4273 4460 1700 Size: 110 555 1908 187 2869 Fragments arranged by size: 2869 1908 555 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 2252 3689 4701 2252 Size: 1437 1012 3180 Fragments arranged by size: 3180 1437 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2293 794 Size: 1499 4130 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2712 2712 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2807 2826 2959 3326 3711 3728 5180 5266 Size: 835 19 133 367 385 17 1452 86 Cuts at: 5266 5278 5624 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 835 385 367 346 133 86 19 17 12 Bsu36I CC'TnA_GG Cuts at: 2013 2013 Size: 5629 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5077 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 3145 3939 3959 3145 Size: 794 20 4815 Fragments arranged by size: 4815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2689 2724 529 Size: 35 2125 35 3434 Fragments arranged by size: 3434 2125 35 35 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2320 4636 809 Size: 507 1004 2316 1802 Fragments arranged by size: 2316 1802 1004 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2343 3076 Size: 54 1425 50 275 18 321 192 733 Cuts at: 3076 3965 5594 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 733 321 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2151 3076 1537 Size: 275 339 925 4090 Fragments arranged by size: 4090 925 339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2571 2883 3559 1353 Size: 1218 312 676 3423 Fragments arranged by size: 3423 1218 676 312 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2234 2252 4216 4590 4736 387 Size: 1064 138 645 18 1964 374 146 1280 Fragments arranged by size: 1964 1280 1064 645 374 146 138 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2351 2381 2585 2907 3692 4289 Size: 393 475 244 30 204 322 785 597 Cuts at: 4289 5076 5344 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 785 597 475 393 322 268 244 204 30 EcoNI CCTnn'n_nnAGG Cuts at: 2834 2834 Size: 5629 EcoO109I rG'GnC_Cy Cuts at: 2248 2839 2248 Size: 591 5038 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 2454 4776 1678 Size: 776 2322 2531 Fragments arranged by size: 2531 2322 776 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2439 2471 4270 4302 4344 Size: 32 135 32 867 32 1799 32 42 Cuts at: 4344 4376 1373 Size: 32 2626 Fragments arranged by size: 2626 1799 867 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KasI G'GCGC_C Cuts at: 2450 2450 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 2706 3228 2706 Size: 522 5107 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 4743 4927 1305 Size: 879 2559 184 2007 Fragments arranged by size: 2559 2007 879 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3587 3946 4105 518 Size: 648 2421 359 159 2042 Fragments arranged by size: 2421 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2278 2404 3045 3722 4870 5115 1606 Size: 672 126 641 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 641 245 126 NarI GG'CG_CC Cuts at: 2451 2451 Size: 5629 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5077 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2545 2987 4532 1132 Size: 97 1316 442 1545 2229 Fragments arranged by size: 2229 1545 1316 442 97 PasI CC'CwG_GG Cuts at: 1348 2303 1348 Size: 955 4674 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2839 2839 Size: 5629 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5629 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2306 2306 Size: 5629 PstI C_TGCA'G Cuts at: 830 2981 830 Size: 2151 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2977 4112 4793 4984 826 Size: 219 1932 1135 681 191 1471 Fragments arranged by size: 1932 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2452 2452 Size: 5629 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 2087 2390 2738 1517 Size: 570 303 348 4408 Fragments arranged by size: 4408 570 348 303 StyI C'CwwG_G Cuts at: 513 1065 2088 2106 2655 3068 513 Size: 552 1023 18 549 413 3074 Fragments arranged by size: 3074 1023 552 549 413 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 3673 3826 4011 4456 325 Size: 1255 2076 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2468 2662 2468 Size: 194 5435 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2904 3437 276 Size: 53 83 186 936 1370 533 2468 Fragments arranged by size: 2468 1370 936 533 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI