Restriction map for
pFN21AB7429
(Circular) (Six-base) MAPSORT of: pFN21AB7429.seq Check: 9641 from: 1 to: 5602
pFN21AB7429 5602 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2430 2430
Size: 5602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AfeI AGC'GCT
Cuts at: 2777 2777
Size: 5602
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2546 1166
Size: 1380 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2843 1184
Size: 1659 3943
AlwNI CAG_nnn'CTG
Cuts at: 2201 3345 4917 2201
Size: 1144 1572 2886
Fragments arranged by size:
2886 1572 1144
ApaI G_GGCC'C
Cuts at: 2600 2630 2600
Size: 30 5572
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2418 2767 2799 2879
Size: 325 217 627 251 380 349 32 80
Cuts at: 2879 2923 4378 618
Size: 44 1455 1842
Fragments arranged by size:
1842 1455 627 380 349 325 251 217
80 44 32
BanII G_rGCy'C
Cuts at: 1391 2600 2630 2921 1391
Size: 1209 30 291 4072
Fragments arranged by size:
4072 1209 291 30
BbeI G_GCGC'C
Cuts at: 2042 2771 2803 2042
Size: 729 32 4841
Fragments arranged by size:
4841 729 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2864 3494 4704 2864
Size: 630 1210 3762
Fragments arranged by size:
3762 1210 630
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 2111 3019 2111
Size: 908 4694
Bme1580I G_kGCm'C
Cuts at: 1790 2416 2600 2630 2832 2882 3663 4819 1790
Size: 626 184 30 202 50 781 1156 2573
Fragments arranged by size:
2573 1156 781 626 202 184 50 30
BmgBI CAC'GTC
Cuts at: 1318 2749 2839 1318
Size: 1431 90 4081
Fragments arranged by size:
4081 1431 90
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2494 2603 3333
Size: 416 53 83 399 24 1077 109 730
Cuts at: 3333 4291 442
Size: 958 1753
Fragments arranged by size:
1753 1077 958 730 416 399 109 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2270 4262 1239
Size: 1016 15 1992 2579
Fragments arranged by size:
2579 1992 1016 15
Bpu10I CC'TnA_GC
Cuts at: 2528 2528
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 3727 4592 4890 2639
Size: 1088 865 298 3351
Fragments arranged by size:
3351 1088 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2705 4265 882
Size: 617 1206 1560 2219
Fragments arranged by size:
2219 1560 1206 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 2516 5596 2516
Size: 3080 2522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 2768 2800 3409
Size: 53 83 186 936 506 729 32 609
Cuts at: 3409 3791 4426 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 729 635 609 506 382 186
83 53 32
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2158 1186
Size: 972 4630
BseYI C'CCAG_C
Cuts at: 2700 4805 5301 2700
Size: 2105 496 3001
Fragments arranged by size:
3001 2105 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 2617 1163
Size: 573 834 47 4148
Fragments arranged by size:
4148 834 573 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 3663 3748 4819 2216
Size: 1447 85 1071 2999
Fragments arranged by size:
2999 1447 1071 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2416 2600 2630 2832 2882 2921
Size: 399 426 200 184 30 202 50 39
Cuts at: 2921 3663 3748 4819 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 202 200 184
85 50 39 30
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2430 2484 2544 2811 2957 844
Size: 1586 54 60 267 146 3489
Fragments arranged by size:
3489 1586 267 146 60 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2105 2239 2339 3489 1394
Size: 429 227 55 134 100 1150 3507
Fragments arranged by size:
3507 1150 429 227 134 100 55
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2714 2816 4246 4433 1700
Size: 110 208 696 102 1430 187 2869
Fragments arranged by size:
2869 1430 696 208 187 110 102
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 2640 1925
Size: 715 4887
BssSI C'ACGA_G
Cuts at: 2279 2675 3662 4674 2279
Size: 396 987 1012 3207
Fragments arranged by size:
3207 1012 987 396
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2886 794
Size: 2092 3510
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2932 3299 3684 3701 5153 5239 5251 5597
Size: 960 367 385 17 1452 86 12 346
Cuts at: 5597 1972
Size: 1977
Fragments arranged by size:
1977 1452 960 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2060 2695 2060
Size: 635 4967
BtgI C'CryG_G
Cuts at: 513 1065 2217 2307 513
Size: 552 1152 90 3808
Fragments arranged by size:
3808 1152 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2403 3118 3912 3932 2403
Size: 715 794 20 4073
Fragments arranged by size:
4073 794 715 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2116 2151 529
Size: 35 1552 35 3980
Fragments arranged by size:
3980 1552 35 35
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DraIII CAC_nnn'GTG
Cuts at: 2502 2812 2502
Size: 310 5292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 2785 2814
Size: 54 1425 50 275 18 816 139 29
Cuts at: 2814 3049 3938 5567 8
Size: 235 889 1629 43
Fragments arranged by size:
1629 1425 889 816 275 235 139 54
50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2095 3532 1353
Size: 687 55 1437 3423
Fragments arranged by size:
3423 1437 687 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2270 3665 4262 5049 5317 1239
Size: 393 623 15 1395 597 787 268 1524
Fragments arranged by size:
1524 1395 787 623 597 393 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2161 2161
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2064 2198 2488 2597 2626 2627 2064
Size: 134 290 109 29 1 5039
Fragments arranged by size:
5039 290 134 109 29 1
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2042 2771 2779 2803 4749 1678
Size: 364 729 8 24 1946 2531
Fragments arranged by size:
2531 1946 729 364 24 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 4243 4275 4317
Size: 32 135 32 1106 32 1533 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 1106 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2038 2767 2799 2038
Size: 729 32 4841
Fragments arranged by size:
4841 729 32
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 4716 4900 1305
Size: 708 2703 184 2007
Fragments arranged by size:
2703 2007 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 10
Size: 54 1425 343 816 2964
Fragments arranged by size:
2964 1425 816 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2546 3560 3919 4078 518
Size: 648 1135 245 1014 359 159 2042
Fragments arranged by size:
2042 1135 1014 648 359 245 159
MspA1I CmG'CkG
Cuts at: 1606 2219 3018 3695 4843 5088 1606
Size: 613 799 677 1148 245 2120
Fragments arranged by size:
2120 1148 799 677 613 245
NaeI GCC'GGC
Cuts at: 2020 2818 2020
Size: 798 4804
NarI GG'CG_CC
Cuts at: 2039 2768 2800 2039
Size: 729 32 4841
Fragments arranged by size:
4841 729 32
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5050
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NgoMIV G'CCGG_C
Cuts at: 2018 2816 2018
Size: 798 4804
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2812 1108
Size: 1704 3898
PfoI T'CCnGG_A
Cuts at: 2345 2345
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2596 2626 2596
Size: 30 5572
PstI C_TGCA'G
Cuts at: 830 2079 2954 830
Size: 1249 875 3478
Fragments arranged by size:
3478 1249 875
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SacII CC_GC'GG
Cuts at: 2220 2220
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SapI GCTCTTCn'nnn_
Cuts at: 2040 2095 2040
Size: 55 5547
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2075 2950 4085 4766 4957 826
Size: 219 1030 875 1135 681 191 1471
Fragments arranged by size:
1471 1135 1030 875 681 219 191
SfoI GGC'GCC
Cuts at: 2040 2769 2801 2040
Size: 729 32 4841
Fragments arranged by size:
4841 729 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3706 4607 4869 820
Size: 197 926 711 1052 901 262 1553
Fragments arranged by size:
1553 1052 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 2156 1517
Size: 639 4963
StyI C'CwwG_G
Cuts at: 513 1065 2067 2319 3041 513
Size: 552 1002 252 722 3074
Fragments arranged by size:
3074 1002 722 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 3629 3646 3799 3984 4429 325
Size: 1255 933 1116 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1116 933 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 2888 1316
Size: 1572 4030
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 2467
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SanDI SexAI SfiI SrfI SwaI