Restriction map for
pFN21AB7426
(Circular) (Six-base) MAPSORT of: pFN21AB7426.seq Check: 6319 from: 1 to: 5941
pFN21AB7426 5941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AfeI AGC'GCT
Cuts at: 2411 3220 2411
Size: 809 5132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2016 3684 5256 2016
Size: 1668 1572 2701
Fragments arranged by size:
2701 1668 1572
ApaI G_GGCC'C
Cuts at: 2670 2670
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2270 2569 2669 2863 3266 1870
Size: 73 93 234 299 100 194 403 4545
Fragments arranged by size:
4545 403 299 234 194 100 93 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 2460 2582 2641 3262
Size: 325 217 627 332 341 122 59 621
Cuts at: 3262 4717 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 621 341 332 325 217
122 59
BanII G_rGCy'C
Cuts at: 1391 2670 2720 2811 3260 1391
Size: 1279 50 91 449 4072
Fragments arranged by size:
4072 1279 449 91 50
BbeI G_GCGC'C
Cuts at: 2464 2645 2464
Size: 181 5760
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3236 928
Size: 971 1337 3633
Fragments arranged by size:
3633 1337 971
BbvCI CC'TCA_GC
Cuts at: 2792 2792
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2059 2580 2629
Size: 107 122 71 631 605 387 521 49
Cuts at: 2629 2665 4552 136
Size: 36 1887 1525
Fragments arranged by size:
1887 1525 631 605 521 387 122 107
71 49 36
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2399 3358 2399
Size: 959 4982
Bme1580I G_kGCm'C
Cuts at: 1790 2670 4002 5158 1790
Size: 880 1332 1156 2573
Fragments arranged by size:
2573 1332 1156 880
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3207 4601 1239
Size: 1968 1394 2579
Fragments arranged by size:
2579 1968 1394
Bpu10I CC'TnA_GC
Cuts at: 2792 2792
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4604 882
Size: 617 1167 1938 2219
Fragments arranged by size:
2219 1938 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 2353 3732 493
Size: 711 1149 1379 2702
Fragments arranged by size:
2702 1379 1149 711
BsaBI GATnn'nnATC
Cuts at: 2189 5935 2189
Size: 3746 2195
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2461 2538 2642 3748
Size: 53 83 186 936 928 77 104 1106
Cuts at: 3748 4130 4765 275
Size: 382 635 1451
Fragments arranged by size:
1451 1106 936 928 635 382 186 104
83 77 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 3183 3213 1178
Size: 30 165 30 639 30 1111 30 3906
Fragments arranged by size:
3906 1111 639 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2590 3204 1186
Size: 1404 614 3923
Fragments arranged by size:
3923 1404 614
BseYI C'CCAG_C
Cuts at: 2728 5144 5640 2728
Size: 2416 496 3029
Fragments arranged by size:
3029 2416 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 2369 1163
Size: 573 614 19 4735
Fragments arranged by size:
4735 614 573 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2714 2843 2859 3391 4152 5180
Size: 275 186 713 129 16 532 761 1028
Cuts at: 5180 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 713 532 275 186 129
16
BsiHKAI G_wGCw'C
Cuts at: 2490 4002 4087 5158 2490
Size: 1512 85 1071 3273
Fragments arranged by size:
3273 1512 1071 85
BsmI GAATG_Cn'
Cuts at: 2711 3452 3545 2711
Size: 741 93 5107
Fragments arranged by size:
5107 741 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2490 2670 2720 2811 3260 4002 4087
Size: 399 700 180 50 91 449 742 85
Cuts at: 4087 5158 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 700 449 399 180 91
85 50
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2434 3095 3828 1394
Size: 429 343 268 661 733 3507
Fragments arranged by size:
3507 733 661 429 343 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 2513 2709 2835 2883 4585 4772
Size: 110 312 391 196 126 48 1702 187
Cuts at: 4772 1700
Size: 2869
Fragments arranged by size:
2869 1702 391 312 196 187 126 110
48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 2472 2733 1925
Size: 547 261 5133
Fragments arranged by size:
5133 547 261
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 2125 2176 2452 2755 1184
Size: 941 51 276 303 4370
Fragments arranged by size:
4370 941 303 276 51
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2057 2496 2676 2685 513
Size: 552 939 53 439 180 9 3769
Fragments arranged by size:
3769 939 552 439 180 53 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2464 487
Size: 683 56 1238 3964
Fragments arranged by size:
3964 1238 683 56
BtsI GCAGTG_nn'
Cuts at: 3159 3457 4251 4271 3159
Size: 298 794 20 4829
Fragments arranged by size:
4829 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3106 3245 3106
Size: 139 5802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2244 2418 2711
Size: 54 1425 50 275 18 414 174 293
Cuts at: 2711 2840 2856 3388 4277 5906 8
Size: 129 16 532 889 1629 43
Fragments arranged by size:
1629 1425 889 532 414 293 275 174
129 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2711 2840 2856 3388 1537
Size: 275 899 129 16 532 4090
Fragments arranged by size:
4090 899 532 275 129 16
EarI CTCTTCn'nnn_
Cuts at: 1353 3871 1353
Size: 2518 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3207 4004 4601 5388 5656 1239
Size: 393 1575 797 597 787 268 1524
Fragments arranged by size:
1575 1524 797 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2548 2666 2548
Size: 118 5823
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2089 2117 2200 2305 2413 2464 2504 2645
Size: 411 28 83 105 108 51 40 141
Cuts at: 2645 2693 3222 5088 1678
Size: 48 529 1866 2531
Fragments arranged by size:
2531 1866 529 411 141 108 105 83
51 48 40 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2460 2641 2460
Size: 181 5760
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 5055 5239 1305
Size: 972 2778 184 2007
Fragments arranged by size:
2778 2007 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 10
Size: 54 1425 343 588 3531
Fragments arranged by size:
3531 1425 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2483 3899 4258 4417 518
Size: 648 1080 237 1416 359 159 2042
Fragments arranged by size:
2042 1416 1080 648 359 237 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2132 2329 2678 2687 2728 2845 3357
Size: 485 41 197 349 9 41 117 512
Cuts at: 3357 4034 5182 5427 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 485 349 245 197
117 41 41 9
NaeI GCC'GGC
Cuts at: 2711 2837 2711
Size: 126 5815
NarI GG'CG_CC
Cuts at: 2461 2642 2461
Size: 181 5760
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2709 2835 2709
Size: 126 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 2840 3388 2840
Size: 548 5393
NspI r_CATG'y
Cuts at: 1132 1229 2212 3299 4844 1132
Size: 97 983 1087 1545 2229
Fragments arranged by size:
2229 1545 1087 983 97
PasI CC'CwG_GG
Cuts at: 1348 2107 2313 1348
Size: 759 206 4976
Fragments arranged by size:
4976 759 206
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2548
Size: 5941
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 2927 3511 2927
Size: 584 5357
PspOMI G'GGCC_C
Cuts at: 2666 2666
Size: 5941
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2728 2728
Size: 5941
SacII CC_GC'GG
Cuts at: 2679 2688 2679
Size: 9 5932
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 3008 4189 5901 3008
Size: 1181 1712 3048
Fragments arranged by size:
3048 1712 1181
SfcI C'TryA_G
Cuts at: 826 1045 3289 4424 5105 5296 826
Size: 219 2244 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2462 2643 2462
Size: 181 5760
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2571 3268 1872
Size: 699 697 4545
Fragments arranged by size:
4545 699 697
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 3380 513
Size: 552 939 1376 3074
Fragments arranged by size:
3074 1376 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2305 3968 3985 4138 4323 4768 325
Size: 1255 725 1663 17 153 185 445 1498
Fragments arranged by size:
1663 1498 1255 725 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2994 3006 3087
Size: 274 80 33 51 1443 1017 12 81
Cuts at: 3087 4187 5899 96
Size: 1100 1712 138
Fragments arranged by size:
1712 1443 1100 1017 274 138 81 80
51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 3183 3215 5901 5933 2042
Size: 32 1109 32 2686 32 2050
Fragments arranged by size:
2686 2050 1109 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2569 3266 1870
Size: 699 697 4545
Fragments arranged by size:
4545 699 697
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI