Restriction map for
pFN21AB7421
(Circular) (Six-base) MAPSORT of: pFN21AB7421.seq Check: 1873 from: 1 to: 5812
pFN21AB7421 5812 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2729 3077 3875 5259 5527 1632
Size: 1097 348 798 1384 268 1917
Fragments arranged by size:
1917 1384 1097 798 348 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2077 4711 5688 1203
Size: 874 2634 977 1327
Fragments arranged by size:
2634 1327 977 874
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 2384 1166
Size: 1218 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaLI G'TGCA_C
Cuts at: 2443 3869 5025 2443
Size: 1426 1156 3230
Fragments arranged by size:
3230 1426 1156
ApoI r'AATT_y
Cuts at: 1493 2486 3121 3347 3499 5608 1493
Size: 993 635 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 635 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2070 4366 160
Size: 872 1038 2296 1606
Fragments arranged by size:
2296 1606 1038 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 2667 3137 1870
Size: 73 724 470 4545
Fragments arranged by size:
4545 724 470 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 3133 4588 618
Size: 325 217 627 908 438 1455 1842
Fragments arranged by size:
1842 1455 908 627 438 325 217
BanII G_rGCy'C
Cuts at: 1391 2612 2843 3055 3131 1391
Size: 1221 231 212 76 4072
Fragments arranged by size:
4072 1221 231 212 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4841
BbvCI CC'TCA_GC
Cuts at: 2342 2342
Size: 5812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3229 3229
Size: 5812
Bme1580I G_kGCm'C
Cuts at: 1790 2447 3873 5029 1790
Size: 657 1426 1156 2573
Fragments arranged by size:
2573 1426 1156 657
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3069 4472 1239
Size: 1830 1403 2579
Fragments arranged by size:
2579 1830 1403
Bpu10I CC'TnA_GC
Cuts at: 2342 2342
Size: 5812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3619 4001 4636 275
Size: 53 83 186 936 2086 382 635 1451
Fragments arranged by size:
2086 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 1186
Size: 1144 4668
BseYI C'CCAG_C
Cuts at: 2497 5015 5511 2497
Size: 2518 496 2798
Fragments arranged by size:
2798 2518 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2742 1163
Size: 573 1006 4233
Fragments arranged by size:
4233 1006 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2447 2843 3055 3873 3958 5029 2447
Size: 396 212 818 85 1071 3230
Fragments arranged by size:
3230 1071 818 396 212 85
BsmI GAATG_Cn'
Cuts at: 2108 3323 3416 2108
Size: 1215 93 4504
Fragments arranged by size:
4504 1215 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2447 2612 2843 3055 3131 3873 3958
Size: 399 657 165 231 212 76 742 85
Cuts at: 3958 5029 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 657 399 231 212 165
85 76
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 2559 3701 5431 2559
Size: 1142 1730 2940
Fragments arranged by size:
2940 1730 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 2582 3167 844
Size: 1574 164 585 3489
Fragments arranged by size:
3489 1574 585 164
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4481 65
Size: 1183 3059 174 1396
Fragments arranged by size:
3059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 2922 96
Size: 2826 2986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5812
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5812
BstYI r'GATC_y
Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807
Size: 1170 367 385 17 1452 86 12 346
Cuts at: 5807 1972
Size: 1977
Fragments arranged by size:
1977 1452 1170 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2224 2224
Size: 5812
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 2028 2049 3116 2028
Size: 21 1067 4724
Fragments arranged by size:
4724 1067 21
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2896 4819 809
Size: 507 1580 1923 1802
Fragments arranged by size:
1923 1802 1580 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 2526 3259
Size: 54 1425 50 275 18 444 252 733
Cuts at: 3259 4148 5777 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 733 444 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 3053 3742 1353
Size: 804 896 689 3423
Fragments arranged by size:
3423 896 804 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 3053 2841
Size: 212 5600
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2729 3069 3077 3875 4472 5259 5527
Size: 393 1097 340 8 798 597 787 268
Cuts at: 5527 1239
Size: 1524
Fragments arranged by size:
1524 1097 798 787 597 393 340 268
8
EcoNI CCTnn'n_nnAGG
Cuts at: 3070 3070
Size: 5812
EcoO109I rG'GnC_Cy
Cuts at: 3063 3083 3063
Size: 20 5792
EcoRI G'AATT_C
Cuts at: 2486 3121 3499 2486
Size: 635 378 4799
Fragments arranged by size:
4799 635 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 5812
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2538 2570 3037 3069 4453
Size: 32 135 32 966 32 467 32 1384
Cuts at: 4453 4485 4527 4559 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1384 966 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3156 3402 669
Size: 988 281 1218 246 3079
Fragments arranged by size:
3079 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2558 4926 5110 1305
Size: 1253 2368 184 2007
Fragments arranged by size:
2368 2007 1253 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2276 2528 10
Size: 54 1425 343 444 252 3294
Fragments arranged by size:
3294 1425 444 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2384 2642 2891 2930 3002 3770
Size: 648 1029 189 258 249 39 72 768
Cuts at: 3770 4129 4288 518
Size: 359 159 2042
Fragments arranged by size:
2042 1029 768 648 359 258 249 189
159 72 39
MspA1I CmG'CkG
Cuts at: 1606 3228 3905 5053 5298 1606
Size: 1622 677 1148 245 2120
Fragments arranged by size:
2120 1622 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5260
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2081 2721 2935 3170 4715 1132
Size: 97 852 640 214 235 1545 2229
Fragments arranged by size:
2229 1545 852 640 235 214 97
PasI CC'CwG_GG
Cuts at: 1348 2097 1348
Size: 749 5063
PciI A'CATG_T
Cuts at: 2077 4711 2077
Size: 2634 3178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3083 3083
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PstI C_TGCA'G
Cuts at: 830 3164 830
Size: 2334 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
SacI G_AGCT'C
Cuts at: 2843 3055 2843
Size: 212 5600
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2148 2181 2790 2823 3022 3160 4295
Size: 219 1103 33 609 33 199 138 1135
Cuts at: 4295 4976 5167 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1103 681 609 219 199 191
138 33 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 2669 3139 1872
Size: 797 470 4545
Fragments arranged by size:
4545 797 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 3066 3251 513
Size: 552 2001 185 3074
Fragments arranged by size:
3074 2001 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 3016 3839 3856 4009 4194 4639
Size: 1255 761 675 823 17 153 185 445
Cuts at: 4639 325
Size: 1498
Fragments arranged by size:
1498 1255 823 761 675 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2331 2922
Size: 274 80 33 51 1443 189 165 591
Cuts at: 2922 4058 5770 96
Size: 1136 1712 138
Fragments arranged by size:
1712 1443 1136 591 274 189 165 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2688 2720 5772 5804 2688
Size: 32 3052 32 2696
Fragments arranged by size:
3052 2696 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2969 1316
Size: 1653 4159
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2434
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 2667 3137 1870
Size: 797 470 4545
Fragments arranged by size:
4545 797 470
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI