Restriction map for
pFN21AB7416
(Circular) (Six-base) MAPSORT of: pFN21AB7416.seq Check: 7286 from: 1 to: 6106
pFN21AB7416 6106 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2175 3916 278
Size: 53 83 186 936 639 1741 2468
Fragments arranged by size:
2468 1741 936 639 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2686 2893 3251 4169 5553 5821 1632
Size: 1054 207 358 918 1384 268 1917
Fragments arranged by size:
1917 1384 1054 918 358 268 207
AfeI AGC'GCT
Cuts at: 2712 2712
Size: 6106
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 3240 3292 5005 5982 1203
Size: 2037 52 1713 977 1327
Fragments arranged by size:
2037 1713 1327 977 52
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 3053 3356 1166
Size: 1887 303 3916
Fragments arranged by size:
3916 1887 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 2131 3000 3079 3136 3266 3849 5421 2131
Size: 869 79 57 130 583 1572 2816
Fragments arranged by size:
2816 1572 869 583 130 79 57
ApaI G_GGCC'C
Cuts at: 2602 2950 2602
Size: 348 5758
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 3415 3641 3793 5902 1493
Size: 1922 226 152 2109 1697
Fragments arranged by size:
2109 1922 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2594 2724 3325 3431 1870
Size: 73 97 554 130 601 106 4545
Fragments arranged by size:
4545 601 554 130 106 97 73
BamHI G'GATC_C
Cuts at: 2633 3270 3436 3803 2633
Size: 637 166 367 4936
Fragments arranged by size:
4936 637 367 166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 2805 3427 4882 618
Size: 325 217 627 444 574 622 1455 1842
Fragments arranged by size:
1842 1455 627 622 574 444 325 217
BanII G_rGCy'C
Cuts at: 1391 2602 2925 2950 3332 3366 3425 1391
Size: 1211 323 25 382 34 59 4072
Fragments arranged by size:
4072 1211 382 323 59 34 25
BbeI G_GCGC'C
Cuts at: 2235 2235
Size: 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BbvCI CC'TCA_GC
Cuts at: 2507 2993 2507
Size: 486 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2155 2189 4297 4331 1903
Size: 34 218 34 2108 34 3678
Fragments arranged by size:
3678 2108 218 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3257 4717 136
Size: 107 122 71 631 605 1585 1460 1525
Fragments arranged by size:
1585 1525 1460 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2602 2950 4167 5323 1790
Size: 812 348 1217 1156 2573
Fragments arranged by size:
2573 1217 1156 812 348
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2857 3109 3837
Size: 416 53 83 399 24 1440 252 728
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1440 958 728 416 399 252 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3008
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 2948 3158 3299 4766 1239
Size: 1394 315 210 141 1467 2579
Fragments arranged by size:
2579 1467 1394 315 210 141
Bpu10I CC'TnA_GC
Cuts at: 2507 2993 2507
Size: 486 5620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 4231 5096 5394 2768
Size: 1463 865 298 3480
Fragments arranged by size:
3480 1463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 3897 493
Size: 711 2105 588 2702
Fragments arranged by size:
2702 2105 711 588
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 2232 2306 2841
Size: 53 83 186 936 639 60 74 535
Cuts at: 2841 3177 3913 4295 4930 275
Size: 336 736 382 635 1451
Fragments arranged by size:
1451 936 736 639 635 535 382 336
186 83 74 60 53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 3384 3414 1178
Size: 30 165 30 1278 30 673 30 3870
Fragments arranged by size:
3870 1278 673 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2225 2228 3029 1186
Size: 1039 3 801 4263
Fragments arranged by size:
4263 1039 801 3
BseYI C'CCAG_C
Cuts at: 2713 2813 3180 3266 3332 5309 5805 2713
Size: 100 367 86 66 1977 496 3014
Fragments arranged by size:
3014 1977 496 367 100 86 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2207 2264 2691 3170 3324 1163
Size: 573 462 9 57 427 479 154 3945
Fragments arranged by size:
3945 573 479 462 427 154 57 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 3556 4317 5345 1540
Size: 275 186 34 1521 761 1028 2301
Fragments arranged by size:
2301 1521 1028 761 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 2658 2925 4167 4252 5323 2658
Size: 267 1242 85 1071 3441
Fragments arranged by size:
3441 1242 1071 267 85
BsmI GAATG_Cn'
Cuts at: 2863 3617 3710 2863
Size: 754 93 5259
Fragments arranged by size:
5259 754 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2209 2297 3283 2209
Size: 88 986 5032
Fragments arranged by size:
5032 986 88
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2602 2658 2925 2950 3332 3366 3425
Size: 399 812 56 267 25 382 34 59
Cuts at: 3425 4167 4252 5323 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 812 742 399 382 267 85
59 56 34 25
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 2658 2853 3201 3995 5725 2658
Size: 195 348 794 1730 3039
Fragments arranged by size:
3039 1730 794 348 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 3993 1394
Size: 429 251 1919 3507
Fragments arranged by size:
3507 1919 429 251
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 4601 4775 65
Size: 1183 1299 2054 174 1396
Fragments arranged by size:
2054 1396 1299 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2353 2445 4750 4937 1700
Size: 110 202 341 92 2305 187 2869
Fragments arranged by size:
2869 2305 341 202 187 110 92
BsrGI T'GTAC_A
Cuts at: 96 3243 96
Size: 3147 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 2795 4166 5178 2795
Size: 1371 1012 3723
Fragments arranged by size:
3723 1371 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 2137 1184
Size: 953 5153
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 3055 3358 2383
Size: 672 303 5131
Fragments arranged by size:
5131 672 303
BstYI r'GATC_y
Cuts at: 1972 2633 2826 3270 3436 3803 4188 4205 5657
Size: 661 193 444 166 367 385 17 1452
Cuts at: 5657 5743 5755 6101 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 661 444 385 367 346 193
166 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2348 2348
Size: 6106
BtgI C'CryG_G
Cuts at: 513 1065 2004 2865 2937 3123 3317 513
Size: 552 939 861 72 186 194 3302
Fragments arranged by size:
3302 939 861 552 194 186 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 3622 4416 4436 2186
Size: 1436 794 20 3856
Fragments arranged by size:
3856 1436 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DraIII CAC_nnn'GTG
Cuts at: 2158 2158
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2543 5113 809
Size: 507 1227 2570 1802
Fragments arranged by size:
2570 1802 1227 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2032 2294
Size: 54 1425 50 275 18 177 25 262
Cuts at: 2294 2385 2770 3402 3553 4442 6071 8
Size: 91 385 632 151 889 1629 43
Fragments arranged by size:
1629 1425 889 632 385 275 262 177
151 91 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2032 3553 1537
Size: 275 220 1521 4090
Fragments arranged by size:
4090 1521 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 3281 4036 1353
Size: 1928 755 3423
Fragments arranged by size:
3423 1928 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2923 2923
Size: 6106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2633 2686 2893 2948 3158 3251 3299
Size: 393 1001 53 207 55 210 93 48
Cuts at: 3299 4169 4766 5553 5821 1239
Size: 870 597 787 268 1524
Fragments arranged by size:
1524 1001 870 787 597 393 268 210
207 93 55 53 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3220 3260 3220
Size: 40 6066
EcoO109I rG'GnC_Cy
Cuts at: 2095 2218 2412 2476 2586 2598 2599 2721 2932
Size: 123 194 64 110 12 1 122 211
Cuts at: 2932 3043 3250 2095
Size: 111 207 4951
Fragments arranged by size:
4951 211 207 194 123 122 111 110
64 12 1
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2131 2235 2714 5253 1678
Size: 453 104 479 2539 2531
Fragments arranged by size:
2539 2531 479 453 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2041 2073 4747 4779 4821
Size: 32 135 32 469 32 2674 32 42
Cuts at: 4821 4853 1373
Size: 32 2626
Fragments arranged by size:
2674 2626 469 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 3450 3696 669
Size: 988 281 1168 344 246 3079
Fragments arranged by size:
3079 1168 988 344 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2231 2231
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 3292 5982 3292
Size: 2690 3416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 10
Size: 54 1425 343 555 3729
Fragments arranged by size:
3729 1425 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3053 3162 3194 3200 3356 4064 4423
Size: 648 1887 109 32 6 156 708 359
Cuts at: 4423 4582 518
Size: 159 2042
Fragments arranged by size:
2042 1887 708 648 359 159 156 109
32 6
MspA1I CmG'CkG
Cuts at: 1606 2266 2506 3184 3277 3371 3522 4199 5347
Size: 660 240 678 93 94 151 677 1148
Cuts at: 5347 5592 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 678 677 660 245 240 151
94 93
NaeI GCC'GGC
Cuts at: 2014 2355 2014
Size: 341 5765
NarI GG'CG_CC
Cuts at: 2232 2232
Size: 6106
NcoI C'CATG_G
Cuts at: 513 1065 2004 3123 513
Size: 552 939 1119 3496
Fragments arranged by size:
3496 1119 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NgoMIV G'CCGG_C
Cuts at: 2012 2353 2012
Size: 341 5765
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2092 3012 3199 3244 3464 5009 1132
Size: 97 863 920 187 45 220 1545 2229
Fragments arranged by size:
2229 1545 920 863 220 187 97 45
PasI CC'CwG_GG
Cuts at: 1348 3383 1348
Size: 2035 4071
PciI A'CATG_T
Cuts at: 3240 5005 3240
Size: 1765 4341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2956 1108
Size: 1848 4258
PfoI T'CCnGG_A
Cuts at: 2916 2925 3168 2916
Size: 9 243 5854
Fragments arranged by size:
5854 243 9
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2412 2476 2721 2932 3043 2095
Size: 317 64 245 211 111 5158
Fragments arranged by size:
5158 317 245 211 111 64
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 2598 2946 2598
Size: 348 5758
PspXI vC'TCGA_Gb
Cuts at: 3325 3325
Size: 6106
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 6074
PstI C_TGCA'G
Cuts at: 830 2362 3458 830
Size: 1532 1096 3478
Fragments arranged by size:
3478 1532 1096
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2506 3184 2506
Size: 678 5428
SacI G_AGCT'C
Cuts at: 2925 2925
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SanDI GG'GwC_CC
Cuts at: 2412 2412
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 2699 4354 6066 2699
Size: 1655 1712 2739
Fragments arranged by size:
2739 1712 1655
SexAI A'CCwGG_T
Cuts at: 2478 2478
Size: 6106
SfcI C'TryA_G
Cuts at: 826 1045 2311 2358 3454 4589 5270 5461 826
Size: 219 1266 47 1096 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 1096 681 219 191 47
SfoI GGC'GCC
Cuts at: 2233 2233
Size: 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 2042 2596 3433 1872
Size: 170 554 837 4545
Fragments arranged by size:
4545 837 554 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 3325 4210 5111 5373 820
Size: 197 926 804 578 885 901 262 1553
Fragments arranged by size:
1553 926 901 885 804 578 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2092 3464 1132
Size: 960 1372 3774
Fragments arranged by size:
3774 1372 960
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2004 3123 3221 3545 513
Size: 552 939 1119 98 324 3074
Fragments arranged by size:
3074 1119 939 552 324 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 3243 4352
Size: 274 80 33 51 1443 720 546 1109
Cuts at: 4352 6064 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 720 546 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 3384 3416 6066 6098 2131
Size: 32 1221 32 2650 32 2139
Fragments arranged by size:
2650 2139 1221 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 3325 1943
Size: 1382 4724
XmaI C'CCGG_G
Cuts at: 1870 2040 2594 3431 1870
Size: 170 554 837 4545
Fragments arranged by size:
4545 837 554 170
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2173 3914 276
Size: 53 83 186 936 639 1741 2468
Fragments arranged by size:
2468 1741 936 639 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
RsrII SacII SapI SfiI SrfI SwaI XcmI