Restriction map for
pFN21AB7415
(Circular) (Six-base) MAPSORT of: pFN21AB7415.seq Check: 3300 from: 1 to: 5668
pFN21AB7415 5668 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 3731 5115 5383 1632
Size: 475 1624 1384 268 1917
Fragments arranged by size:
1917 1624 1384 475 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2408 2440 2408
Size: 32 5636
AlwNI CAG_nnn'CTG
Cuts at: 2174 3411 4983 2174
Size: 1237 1572 2859
Fragments arranged by size:
2859 1572 1237
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2182 2251 2277 2298 2977 3203 3355 5464
Size: 689 69 26 21 679 226 152 2109
Cuts at: 5464 1493
Size: 1697
Fragments arranged by size:
2109 1697 689 679 226 152 69 26
21
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 2993 1870
Size: 73 726 324 4545
Fragments arranged by size:
4545 726 324 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 4444 618
Size: 325 217 627 1202 1455 1842
Fragments arranged by size:
1842 1455 1202 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2821 2987 1391
Size: 1430 166 4072
Fragments arranged by size:
4072 1430 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 928
Size: 971 676 4021
Fragments arranged by size:
4021 971 676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BfrBI ATG'CAT
Cuts at: 2393 2393
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2519 2616 3729 4885 1790
Size: 729 97 1113 1156 2573
Fragments arranged by size:
2573 1156 1113 729 97
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2766 1056
Size: 1710 3958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 4328 1239
Size: 894 2195 2579
Fragments arranged by size:
2579 2195 894
Bpu10I CC'TnA_GC
Cuts at: 2160 2160
Size: 5668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 3793 4658 4956 2399
Size: 1394 865 298 3111
Fragments arranged by size:
3111 1394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2115 2310 4331 882
Size: 617 616 195 2021 2219
Fragments arranged by size:
2219 2021 617 616 195
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 1178
Size: 30 165 30 1005 30 4408
Fragments arranged by size:
4408 1005 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 1186
Size: 1210 4458
BseYI C'CCAG_C
Cuts at: 2518 4871 5367 2518
Size: 2353 496 2819
Fragments arranged by size:
2819 2353 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2821 3729 3814 4885 2821
Size: 908 85 1071 3604
Fragments arranged by size:
3604 1071 908 85
BsmI GAATG_Cn'
Cuts at: 2393 3179 3272 2393
Size: 786 93 4789
Fragments arranged by size:
4789 786 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2019 2019
Size: 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2519 2616 2821 2987 3729 3814 4885
Size: 399 729 97 205 166 742 85 1071
Cuts at: 4885 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 729 399 205 166 97
85
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 2113 3557 5287 2113
Size: 1444 1730 2494
Fragments arranged by size:
2494 1730 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 844
Size: 2179 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2638 794
Size: 1844 3824
BstBI TT'CG_AA
Cuts at: 2296 4472 2296
Size: 2176 3492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 2649 2447
Size: 202 5466
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4177
Fragments arranged by size:
4177 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2843 2883 3184 3978 3998 2843
Size: 40 301 794 20 4513
Fragments arranged by size:
4513 794 301 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2547 2972 2547
Size: 425 5243
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2260 3115
Size: 54 1425 50 275 18 378 52 855
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 378 275 54 52
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 3598 1353
Size: 1473 772 3423
Fragments arranged by size:
3423 1473 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2819 2819
Size: 5668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2133 3731 4328 5115 5383 1239
Size: 393 475 26 1598 597 787 268 1524
Fragments arranged by size:
1598 1524 787 597 475 393 268 26
EcoO109I rG'GnC_Cy
Cuts at: 2156 2156
Size: 5668
EcoRI G'AATT_C
Cuts at: 2277 2977 3355 2277
Size: 700 378 4590
Fragments arranged by size:
4590 700 378
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2041 2073 2129 2161 2790
Size: 32 135 32 469 32 56 32 629
Cuts at: 2790 2822 4309 4341 4383 4415 1373
Size: 32 1487 32 42 32 2626
Fragments arranged by size:
2626 1487 629 469 135 56 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2262 10
Size: 54 1425 343 378 52 3416
Fragments arranged by size:
3416 1425 378 343 54 52
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 3626 3985 4144 518
Size: 648 1279 1181 359 159 2042
Fragments arranged by size:
2042 1279 1181 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4177
Fragments arranged by size:
4177 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 2762 1052
Size: 1710 3958
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NsiI A_TGCA'T
Cuts at: 2395 2395
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2074 3026 4571 1132
Size: 97 845 952 1545 2229
Fragments arranged by size:
2229 1545 952 845 97
PasI CC'CwG_GG
Cuts at: 1348 2649 1348
Size: 1301 4367
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2408 2440 3737 3769 5250 5282 2408
Size: 32 1297 32 1481 32 2794
Fragments arranged by size:
2794 1481 1297 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2156 2156
Size: 5668
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SacI G_AGCT'C
Cuts at: 2821 2821
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SapI GCTCTTCn'nnn_
Cuts at: 2826 2826
Size: 5668
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 3016 4151 4832 5023 826
Size: 219 1971 1135 681 191 1471
Fragments arranged by size:
1971 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 3772 4673 4935 820
Size: 197 926 435 1394 901 262 1553
Fragments arranged by size:
1553 1394 926 901 435 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 2074 3026 1132
Size: 942 952 3774
Fragments arranged by size:
3774 952 942
SspI AAT'ATT
Cuts at: 5 52 2878 3592 5
Size: 47 2826 714 2081
Fragments arranged by size:
2826 2081 714 47
StuI AGG'CCT
Cuts at: 1517 2376 1517
Size: 859 4809
StyI C'CwwG_G
Cuts at: 513 1065 2004 3107 513
Size: 552 939 1103 3074
Fragments arranged by size:
3074 1103 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2270 2270
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI