Restriction map for
pFN21AB7413
(Circular) (Six-base) MAPSORT of: pFN21AB7413.seq Check: 1730 from: 1 to: 5860
pFN21AB7413 5860 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2667 2667
Size: 5860
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2242 3203 1937
Size: 305 961 4594
Fragments arranged by size:
4594 961 305
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3028 3923 5307 5575 1632
Size: 1396 895 1384 268 1917
Fragments arranged by size:
1917 1396 1384 895 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 2803 4589 2803
Size: 1786 4074
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2545 2762 2795 3068 3603 5175 2545
Size: 217 33 273 535 1572 3230
Fragments arranged by size:
3230 1572 535 273 217 33
ApaI G_GGCC'C
Cuts at: 2950 2950
Size: 5860
ApaLI G'TGCA_C
Cuts at: 2556 3917 5073 2556
Size: 1361 1156 3343
Fragments arranged by size:
3343 1361 1156
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2599 2880 3185 1870
Size: 73 656 281 305 4545
Fragments arranged by size:
4545 656 305 281 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2512 2701 3015 3181 4636
Size: 325 217 627 725 189 314 166 1455
Cuts at: 4636 618
Size: 1842
Fragments arranged by size:
1842 1455 725 627 325 314 217 189
166
BanII G_rGCy'C
Cuts at: 1391 2715 2890 2950 3179 1391
Size: 1324 175 60 229 4072
Fragments arranged by size:
4072 1324 229 175 60
BbeI G_GCGC'C
Cuts at: 2705 2705
Size: 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 3120 928
Size: 971 564 657 3668
Fragments arranged by size:
3668 971 657 564
BbvCI CC'TCA_GC
Cuts at: 2914 2914
Size: 5860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4051 4085 1903
Size: 34 2114 34 3678
Fragments arranged by size:
3678 2114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 2472 1548
Size: 924 4936
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2631 4471 136
Size: 107 122 71 631 605 959 1840 1525
Fragments arranged by size:
1840 1525 959 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 2292 2588 3277 2292
Size: 296 689 4875
Fragments arranged by size:
4875 689 296
Bme1580I G_kGCm'C
Cuts at: 1790 2560 2950 3018 3921 5077 1790
Size: 770 390 68 903 1156 2573
Fragments arranged by size:
2573 1156 903 770 390 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2074 3591 4549
Size: 416 53 83 399 24 657 1517 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1517 958 657 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2423 2423
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 2729 2745 4520 1239
Size: 1271 219 16 1775 2579
Fragments arranged by size:
2579 1775 1271 219 16
Bpu10I CC'TnA_GC
Cuts at: 2517 2914 2517
Size: 397 5463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2503 3651 493
Size: 711 1299 1148 2702
Fragments arranged by size:
2702 1299 1148 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2702 3667 4049 4684
Size: 53 83 186 936 1169 965 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1451 1169 965 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2335 1186
Size: 1149 4711
BseYI C'CCAG_C
Cuts at: 2221 2538 2755 3011 5063 5559 2221
Size: 317 217 256 2052 496 2522
Fragments arranged by size:
2522 2052 496 317 256 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 2336 1163
Size: 573 436 164 4687
Fragments arranged by size:
4687 573 436 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2560 2890 3921 4006 5077 2560
Size: 330 1031 85 1071 3343
Fragments arranged by size:
3343 1071 1031 330 85
BsmI GAATG_Cn'
Cuts at: 2146 3371 3464 2146
Size: 1225 93 4542
Fragments arranged by size:
4542 1225 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2560 2715 2890 2950 3018 3179 3921
Size: 399 770 155 175 60 68 161 742
Cuts at: 3921 4006 5077 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 770 742 399 175 161 155
85 68 60
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2667 3215 844
Size: 1823 548 3489
Fragments arranged by size:
3489 1823 548
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 2368 96
Size: 2272 3588
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 2781 3920 4932 2781
Size: 1139 1012 3709
Fragments arranged by size:
3709 1139 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 2663 2663
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2107 2388 3190 3557 3942 3959 5411 5497
Size: 135 281 802 367 385 17 1452 86
Cuts at: 5497 5509 5855 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 802 385 367 346 281 135
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2691 513
Size: 552 939 687 3682
Fragments arranged by size:
3682 939 687 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4170 4190 3376
Size: 794 20 5046
Fragments arranged by size:
5046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 3307 4196
Size: 54 1425 50 275 18 302 1175 889
Cuts at: 4196 5825 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1175 889 302 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 2414 3147 3790 1353
Size: 663 398 733 643 3423
Fragments arranged by size:
3423 733 663 643 398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2880 4447 4821 4967 387
Size: 1064 138 1291 1567 374 146 1280
Fragments arranged by size:
1567 1291 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2888 2888
Size: 5860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2729 2745 3028 3923 4520 5307
Size: 393 878 219 16 283 895 597 787
Cuts at: 5307 5575 1239
Size: 268 1524
Fragments arranged by size:
1524 895 878 787 597 393 283 268
219 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3056 3080 3056
Size: 24 5836
EcoO109I rG'GnC_Cy
Cuts at: 2325 2749 2792 2325
Size: 424 43 5393
Fragments arranged by size:
5393 424 43
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2545 2705 5007 1678
Size: 867 160 2302 2531
Fragments arranged by size:
2531 2302 867 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2789 2821 4501 4533 4575
Size: 32 135 32 1217 32 1680 32 42
Cuts at: 4575 4607 1373
Size: 32 2626
Fragments arranged by size:
2626 1680 1217 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2982 748
Size: 2234 3626
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KasI G'GCGC_C
Cuts at: 2701 2701
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2134 10
Size: 54 1425 343 302 3736
Fragments arranged by size:
3736 1425 343 302 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2661 3818 4177 4336 518
Size: 648 1299 196 1157 359 159 2042
Fragments arranged by size:
2042 1299 1157 648 359 196 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2296 3065 3276 3953 5101 5346 1606
Size: 606 84 769 211 677 1148 245 2120
Fragments arranged by size:
2120 1148 769 677 606 245 211 84
NarI GG'CG_CC
Cuts at: 2702 2702
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4369
Fragments arranged by size:
4369 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2438 1348
Size: 1090 4770
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2762 1108
Size: 1654 4206
PfoI T'CCnGG_A
Cuts at: 2745 2745
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PmlI CAC'GTG
Cuts at: 2503 2503
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 2749
Size: 5860
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2946 2946
Size: 5860
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2296 3065 2296
Size: 769 5091
SacI G_AGCT'C
Cuts at: 2890 2890
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SapI GCTCTTCn'nnn_
Cuts at: 2016 3147 2016
Size: 1131 4729
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2252 3208 4343 5024 5215 826
Size: 219 1207 956 1135 681 191 1471
Fragments arranged by size:
1471 1207 1135 956 681 219 191
SfoI GGC'GCC
Cuts at: 2703 2703
Size: 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 2601 2882 3187 1872
Size: 729 281 305 4545
Fragments arranged by size:
4545 729 305 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 2135 3299 513
Size: 552 939 131 1164 3074
Fragments arranged by size:
3074 1164 939 552 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2368 4106 5818
Size: 274 80 33 51 1443 391 1738 1712
Cuts at: 5818 96
Size: 138
Fragments arranged by size:
1738 1712 1443 391 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 5820 5852 2136
Size: 32 3652 32 2144
Fragments arranged by size:
3652 2144 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2267 2267
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 2599 2880 3185 1870
Size: 729 281 305 4545
Fragments arranged by size:
4545 729 305 281
XmnI GAAnn'nnTTC
Cuts at: 2124 3989 2124
Size: 1865 3995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI