Restriction map for
pFN21AB7411
(Circular) (Six-base) MAPSORT of: pFN21AB7411.seq Check: 7678 from: 1 to: 5647
pFN21AB7411 5647 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2607 2607
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2552 2990 1937
Size: 615 438 4594
Fragments arranged by size:
4594 615 438
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 3710 5094 5362 1632
Size: 431 1647 1384 268 1917
Fragments arranged by size:
1917 1647 1384 431 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2330 1166
Size: 1164 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2414 1184
Size: 1230 4417
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2171 2956 3182 3334 5443 1493
Size: 678 785 226 152 2109 1697
Fragments arranged by size:
2109 1697 785 678 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2709 2968 4423 618
Size: 325 217 627 922 259 1455 1842
Fragments arranged by size:
1842 1455 922 627 325 259 217
BanII G_rGCy'C
Cuts at: 1391 2040 2966 1391
Size: 649 926 4072
Fragments arranged by size:
4072 926 649
BbeI G_GCGC'C
Cuts at: 2713 2713
Size: 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 2145 2646 1548
Size: 597 501 4549
Fragments arranged by size:
4549 597 501
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2437 3708 4864 1790
Size: 647 1271 1156 2573
Fragments arranged by size:
2573 1271 1156 647
BmgBI CAC'GTC
Cuts at: 1318 2899 1318
Size: 1581 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4307 1239
Size: 3068 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2276 3772 4637 4935 2276
Size: 1496 865 298 2988
Fragments arranged by size:
2988 1496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2710 3454 3836 4471
Size: 53 83 186 936 1177 744 382 635
Cuts at: 4471 275
Size: 1451
Fragments arranged by size:
1451 1177 936 744 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2502 4080 4478 4752 4899 5310 2502
Size: 1578 398 274 147 411 2839
Fragments arranged by size:
2839 1578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 2447 4850 5346 2447
Size: 2403 496 2748
Fragments arranged by size:
2748 2403 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2308 1163
Size: 573 572 4502
Fragments arranged by size:
4502 573 572
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2437 2966 3708 3793 4864 1391
Size: 399 250 397 529 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 529 399 397 250 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 3002 844
Size: 1763 395 3489
Fragments arranged by size:
3489 1763 395
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 2636 96
Size: 2540 3107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2553 2553
Size: 5647
Bsu36I CC'TnA_GG
Cuts at: 2885 2885
Size: 5647
BtgI C'CryG_G
Cuts at: 513 1065 2764 513
Size: 552 1699 3396
Fragments arranged by size:
3396 1699 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 2364 3163 3957 3977 2325
Size: 39 799 794 20 3995
Fragments arranged by size:
3995 799 794 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2525 2560 529
Size: 35 1961 35 3616
Fragments arranged by size:
3616 1961 35 35
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2412 3094
Size: 54 1425 50 275 18 552 30 682
Cuts at: 3094 3983 5612 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 682 552 275 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2236 3577 1353
Size: 743 140 1341 3423
Fragments arranged by size:
3423 1341 743 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 3710 4307 5094 5362 1239
Size: 393 431 1647 597 787 268 1524
Fragments arranged by size:
1647 1524 787 597 431 393 268
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2713 4794 1678
Size: 1035 2081 2531
Fragments arranged by size:
2531 2081 1035
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373
Size: 32 135 32 2716 32 42 32 2626
Fragments arranged by size:
2716 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2854 2991 3237 669
Size: 988 281 916 137 246 3079
Fragments arranged by size:
3079 988 916 281 246 137
HindIII A'AGCT_T
Cuts at: 748 2075 748
Size: 1327 4320
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2709 2709
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 2414 10
Size: 54 1425 343 552 30 3243
Fragments arranged by size:
3243 1425 552 343 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2183 2330 2567 2675 3605 3964
Size: 648 843 174 147 237 108 930 359
Cuts at: 3964 4123 518
Size: 159 2042
Fragments arranged by size:
2042 930 843 648 359 237 174 159
147 108
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NarI GG'CG_CC
Cuts at: 2710 2710
Size: 5647
NcoI C'CATG_G
Cuts at: 513 1065 2764 513
Size: 552 1699 3396
Fragments arranged by size:
3396 1699 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2470 3005 4550 1132
Size: 97 1241 535 1545 2229
Fragments arranged by size:
2229 1545 1241 535 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 2312 3895 5607 2312
Size: 1583 1712 2352
Fragments arranged by size:
2352 1712 1583
SfcI C'TryA_G
Cuts at: 826 1045 2878 2995 4130 4811 5002 826
Size: 219 1833 117 1135 681 191 1471
Fragments arranged by size:
1833 1471 1135 681 219 191 117
SfoI GGC'GCC
Cuts at: 2711 2711
Size: 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 3751 4652 4914 820
Size: 197 926 312 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 312 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2137 2478 2764 3086 513
Size: 552 1072 341 286 322 3074
Fragments arranged by size:
3074 1072 552 341 322 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 2310 2636
Size: 274 80 33 51 1443 210 123 326
Cuts at: 2636 2683 3893 5605 96
Size: 47 1210 1712 138
Fragments arranged by size:
1712 1443 1210 326 274 210 138 123
80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 2341 1316
Size: 1025 4622
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI