Restriction map for
pFN21AB7410
(Circular) (Six-base) MAPSORT of: pFN21AB7410.seq Check: 3939 from: 1 to: 5857
pFN21AB7410 5857 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2818 2818
Size: 5857
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 3920 5304 5572 1632
Size: 1011 1277 1384 268 1917
Fragments arranged by size:
1917 1384 1277 1011 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 2651 4756 5733 1203
Size: 1448 2105 977 1327
Fragments arranged by size:
2105 1448 1327 977
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 5825
AlwNI CAG_nnn'CTG
Cuts at: 2152 3600 5172 2152
Size: 1448 1572 2837
Fragments arranged by size:
2837 1572 1448
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 2507 3166 3392 3544 5653 1493
Size: 1014 659 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 659 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 3182 1870
Size: 73 71 1168 4545
Fragments arranged by size:
4545 1168 73 71
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2544 3178 4633 618
Size: 325 217 627 232 525 634 1455 1842
Fragments arranged by size:
1842 1455 634 627 525 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2199 2321 3176 1391
Size: 808 122 855 4072
Fragments arranged by size:
4072 855 808 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2116 2241 2788 928
Size: 971 217 125 547 3997
Fragments arranged by size:
3997 971 547 217 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2435 2980 3749 4959 2435
Size: 545 769 1210 3333
Fragments arranged by size:
3333 1210 769 545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2693 5852 2693
Size: 3159 2698
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2549 3918 5074 1790
Size: 232 527 1369 1156 2573
Fragments arranged by size:
2573 1369 1156 527 232
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 4517 1239
Size: 998 2280 2579
Fragments arranged by size:
2579 2280 998
Bpu10I CC'TnA_GC
Cuts at: 2315 2315
Size: 5857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 4520 882
Size: 617 518 2503 2219
Fragments arranged by size:
2503 2219 617 518
BsaAI yAC'GTr
Cuts at: 493 1204 3648 493
Size: 711 2444 2702
Fragments arranged by size:
2702 2444 711
BsaBI GATnn'nnATC
Cuts at: 2612 5851 2612
Size: 3239 2618
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2572 4290 4688 4962 5109 5520 2572
Size: 1718 398 274 147 411 2909
Fragments arranged by size:
2909 1718 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2235 2265 2909 2939 3041
Size: 30 165 30 832 30 644 30 102
Cuts at: 3041 3071 1178
Size: 30 3964
Fragments arranged by size:
3964 832 644 165 102 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2522 2764 1186
Size: 1021 315 242 4279
Fragments arranged by size:
4279 1021 315 242
BseYI C'CCAG_C
Cuts at: 5060 5556 5060
Size: 496 5361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2320 2913 1163
Size: 573 584 593 4107
Fragments arranged by size:
4107 593 584 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2321 3918 4003 5074 2199
Size: 122 1597 85 1071 2982
Fragments arranged by size:
2982 1597 1071 122 85
BsmI GAATG_Cn'
Cuts at: 2944 3368 3461 2944
Size: 424 93 5340
Fragments arranged by size:
5340 424 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2199 2321 2549 3176 3918 4003
Size: 399 232 177 122 228 627 742 85
Cuts at: 4003 5074 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 627 399 232 228 177
122 85
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2818 3212 844
Size: 1974 394 3489
Fragments arranged by size:
3489 1974 394
BsrBI CCG'CTC
Cuts at: 1394 1823 3744 1394
Size: 429 1921 3507
Fragments arranged by size:
3507 1921 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2859 3060 4352 4526 65
Size: 1183 1611 201 1292 174 1396
Fragments arranged by size:
1611 1396 1292 1183 201 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 2758 3917 4929 2758
Size: 1159 1012 3686
Fragments arranged by size:
3686 1159 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5857
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 2095 1184
Size: 911 4946
BstYI r'GATC_y
Cuts at: 1972 2248 2693 3187 3554 3939 3956 5408 5494
Size: 276 445 494 367 385 17 1452 86
Cuts at: 5494 5506 5852 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 494 445 385 367 346 276
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 2742 513
Size: 552 939 348 390 3628
Fragments arranged by size:
3628 939 552 390 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 2720 3373 4167 4187 2224
Size: 496 653 794 20 3894
Fragments arranged by size:
3894 794 653 496 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2147 4864 809
Size: 507 831 2717 1802
Fragments arranged by size:
2717 1802 831 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 3304 4193
Size: 54 1425 50 275 18 313 1161 889
Cuts at: 4193 5822 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1161 889 313 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2564 2619 3787 1353
Size: 716 495 55 1168 3423
Fragments arranged by size:
3423 1168 716 495 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4444 4818 4964 387
Size: 1064 138 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2197 2319 2197
Size: 122 5735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2643 3920 4517 5304 5572 1239
Size: 393 605 406 1277 597 787 268 1524
Fragments arranged by size:
1524 1277 787 605 597 406 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2050 2149 2636 2050
Size: 99 487 5271
Fragments arranged by size:
5271 487 99
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373
Size: 32 135 32 2926 32 42 32 2626
Fragments arranged by size:
2926 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2800 2820 2907 3022 4971 5155 1305
Size: 1495 20 87 115 1949 184 2007
Fragments arranged by size:
2007 1949 1495 184 115 87 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4035
Fragments arranged by size:
4035 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3815 4174 4333 518
Size: 648 2649 359 159 2042
Fragments arranged by size:
2649 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2897 3273 3950 5098 5343 1606
Size: 1291 376 677 1148 245 2120
Fragments arranged by size:
2120 1291 1148 677 376 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2742 513
Size: 552 939 738 3628
Fragments arranged by size:
3628 939 738 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2407 2655 3215 4760 1132
Size: 97 1178 248 560 1545 2229
Fragments arranged by size:
2229 1545 1178 560 248 97
PasI CC'CwG_GG
Cuts at: 1348 2053 1348
Size: 705 5152
PciI A'CATG_T
Cuts at: 2651 4756 2651
Size: 2105 3752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 2304 1108
Size: 1044 152 4661
Fragments arranged by size:
4661 1044 152
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3926 3958 5439 5471 3041
Size: 32 853 32 1481 32 3427
Fragments arranged by size:
3427 1481 853 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2149
Size: 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 2493 3427 2493
Size: 934 4923
PstI C_TGCA'G
Cuts at: 830 2204 2219 2268 2675 3209 830
Size: 1374 15 49 407 534 3478
Fragments arranged by size:
3478 1374 534 407 49 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2897 2897
Size: 5857
SacI G_AGCT'C
Cuts at: 2199 2321 2199
Size: 122 5735
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SapI GCTCTTCn'nnn_
Cuts at: 2069 2619 2069
Size: 550 5307
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 2396 4105 5817 2396
Size: 1709 1712 2436
Fragments arranged by size:
2436 1712 1709
SfcI C'TryA_G
Cuts at: 826 1045 2200 2215 2264 2671 3205 4340 5021
Size: 219 1155 15 49 407 534 1135 681
Cuts at: 5021 5212 826
Size: 191 1471
Fragments arranged by size:
1471 1155 1135 681 534 407 219 191
49 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 3184 1872
Size: 1312 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 2407 3215 1132
Size: 1275 808 3774
Fragments arranged by size:
3774 1275 808
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 2004 2742 3296 513
Size: 552 939 738 554 3074
Fragments arranged by size:
3074 939 738 554 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 3884 3901 4054 4239 4684 325
Size: 1255 522 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 522 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 4103 5815
Size: 274 80 33 51 1443 417 1709 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1709 1443 417 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2814 2846 5817 5849 2814
Size: 32 2971 32 2822
Fragments arranged by size:
2971 2822 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 3182 1870
Size: 1312 4545
XmnI GAAnn'nnTTC
Cuts at: 2628 2942 3986 2628
Size: 314 1044 4499
Fragments arranged by size:
4499 1044 314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI