Restriction map for
pFN21AB7408
(Circular) (Six-base) MAPSORT of: pFN21AB7408.seq Check: 4105 from: 1 to: 5887
pFN21AB7408 5887 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 2400 3208 2400
Size: 808 5079
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3950 5334 5602 1632
Size: 2318 1384 268 1917
Fragments arranged by size:
2318 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 2409 2498 1166
Size: 1243 89 4555
Fragments arranged by size:
4555 1243 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3630 5202 3630
Size: 1572 4315
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2557 2900 2923 3196 3422 3574 5683 1493
Size: 1064 343 23 273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 343 273 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 2792 4441 160
Size: 872 1760 1649 1606
Fragments arranged by size:
1760 1649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2400 3208 4663 618
Size: 325 217 627 409 204 808 1455 1842
Fragments arranged by size:
1842 1455 808 627 409 325 217 204
BanII G_rGCy'C
Cuts at: 1391 2397 3206 1391
Size: 1006 809 4072
Fragments arranged by size:
4072 1006 809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2174 2285 928
Size: 971 275 111 4530
Fragments arranged by size:
4530 971 275 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2141 2175 2252 2286 4078 4112 1903
Size: 34 204 34 77 34 1792 34 3678
Fragments arranged by size:
3678 1792 204 77 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2419 3037 5882 2419
Size: 618 2845 2424
Fragments arranged by size:
2845 2424 618
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3948 5104 1790
Size: 348 1810 1156 2573
Fragments arranged by size:
2573 1810 1156 348
BmgBI CAC'GTC
Cuts at: 1318 2407 2500 1318
Size: 1089 93 4705
Fragments arranged by size:
4705 1089 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3618 4576 442
Size: 416 53 83 399 24 2201 958 1753
Fragments arranged by size:
2201 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2547 2646 2547
Size: 99 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2163 2274 4547 1239
Size: 924 111 2273 2579
Fragments arranged by size:
2579 2273 924 111
Bpu10I CC'TnA_GC
Cuts at: 2486 2486
Size: 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3018 3029 3154 4012 4877 5175 3018
Size: 11 125 858 865 298 3730
Fragments arranged by size:
3730 865 858 298 125 11
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 2139 2250 4550 882
Size: 617 567 73 111 2300 2219
Fragments arranged by size:
2300 2219 617 567 111 73
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2384 2414 1178
Size: 30 165 30 981 30 4651
Fragments arranged by size:
4651 981 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5887
BseYI C'CCAG_C
Cuts at: 5090 5586 5090
Size: 496 5391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2747 1163
Size: 573 1011 4303
Fragments arranged by size:
4303 1011 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2397 3206 3948 4033 5104 1391
Size: 399 348 259 809 742 85 1071 2174
Fragments arranged by size:
2174 1071 809 742 399 348 259 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 2340 3774 1394
Size: 429 295 222 1434 3507
Fragments arranged by size:
3507 1434 429 295 222
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 4531 4718 1700
Size: 110 219 2502 187 2869
Fragments arranged by size:
2869 2502 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 2872 96
Size: 2776 3111
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2085 2196 2307 794
Size: 1291 111 111 4374
Fragments arranged by size:
4374 1291 111 111
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2411 2411
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2419 2470 3037 3217 3584 3969 3986 5438
Size: 447 51 567 180 367 385 17 1452
Cuts at: 5438 5524 5536 5882 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 567 447 385 367 346 180
86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2410 513
Size: 552 939 406 3990
Fragments arranged by size:
3990 939 552 406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2314 2804 3403 4197 4217 2203
Size: 111 490 599 794 20 3873
Fragments arranged by size:
3873 794 599 490 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 2928 3191 2928
Size: 263 5624
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2796 3054 3334
Size: 54 1425 50 275 18 966 258 280
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 966 889 280 275 258 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2044 3145 3817 1353
Size: 691 1101 672 3423
Fragments arranged by size:
3423 1101 691 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2274 3950 4547 5334 5602 1239
Size: 393 531 111 1676 597 787 268 1524
Fragments arranged by size:
1676 1524 787 597 531 393 268 111
EcoNI CCTnn'n_nnAGG
Cuts at: 2107 2218 2329 2107
Size: 111 111 5665
Fragments arranged by size:
5665 111 111
EcoRI G'AATT_C
Cuts at: 2557 3196 3574 2557
Size: 639 378 4870
Fragments arranged by size:
4870 639 378
EcoRV GAT'ATC
Cuts at: 2827 2827
Size: 5887
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2250 2361 2385 5034 1678
Size: 572 111 24 2649 2531
Fragments arranged by size:
2649 2531 572 111 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2816 2848 4528 4560 4602
Size: 32 135 32 1244 32 1680 32 42
Cuts at: 4602 4634 1373
Size: 32 2626
Fragments arranged by size:
2626 1680 1244 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2597 3231 3477 669
Size: 988 281 659 634 246 3079
Fragments arranged by size:
3079 988 659 634 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 2404 3212 2404
Size: 808 5079
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2798 10
Size: 54 1425 343 966 3099
Fragments arranged by size:
3099 1425 966 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2409 2480 2498 3084 3845 4204 4363
Size: 648 1243 71 18 586 761 359 159
Cuts at: 4363 518
Size: 2042
Fragments arranged by size:
2042 1243 761 648 586 359 159 71
18
MspA1I CmG'CkG
Cuts at: 1606 3303 3980 5128 5373 1606
Size: 1697 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 245
NaeI GCC'GGC
Cuts at: 2031 2031
Size: 5887
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NgoMIV G'CCGG_C
Cuts at: 2029 2029
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 3245 4790 1132
Size: 97 2016 1545 2229
Fragments arranged by size:
2229 2016 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 2222 2333 3239 830
Size: 1392 111 906 3478
Fragments arranged by size:
3478 1392 906 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 2107 2218 2329 3235 4370 5051 5242
Size: 219 1062 111 111 906 1135 681 191
Cuts at: 5242 826
Size: 1471
Fragments arranged by size:
1471 1135 1062 906 681 219 191 111
111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3008 3033 3169 3991 4892 5154
Size: 197 926 1065 25 136 822 901 262
Cuts at: 5154 820
Size: 1553
Fragments arranged by size:
1553 1065 926 901 822 262 197 136
25
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 2734 3019 3811 5
Size: 47 2682 285 792 2081
Fragments arranged by size:
2682 2081 792 285 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2004 3326 513
Size: 552 939 1322 3074
Fragments arranged by size:
3074 1322 939 552
SwaI ATTT'AAAT
Cuts at: 2928 2928
Size: 5887
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3914 3931 4084 4269 4714 325
Size: 1255 2334 17 153 185 445 1498
Fragments arranged by size:
2334 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2872 4133 5845
Size: 274 80 33 51 1443 895 1261 1712
Cuts at: 5845 96
Size: 138
Fragments arranged by size:
1712 1443 1261 895 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 2592 1316
Size: 1276 4611
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 4016 4016
Size: 5887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI XcmI