Restriction map for
pFN21AB7407
(Circular) (Six-base) MAPSORT of: pFN21AB7407.seq Check: 7737 from: 1 to: 5344
pFN21AB7407 5344 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2121 2687 1937
Size: 184 566 4594
Fragments arranged by size:
4594 566 184
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 3407 4791 5059 1632
Size: 890 885 1384 268 1917
Fragments arranged by size:
1917 1384 890 885 268
AfeI AGC'GCT
Cuts at: 2336 2336
Size: 5344
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5344
AlwNI CAG_nnn'CTG
Cuts at: 3087 4659 3087
Size: 1572 3772
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2653 2879 3031 5140 1493
Size: 1160 226 152 2109 1697
Fragments arranged by size:
2109 1697 1160 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2363 2584 2669 1870
Size: 73 420 221 85 4545
Fragments arranged by size:
4545 420 221 85 73
BamHI G'GATC_C
Cuts at: 2674 3041 2674
Size: 367 4977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2236 2359 2665 4120
Size: 325 217 627 221 228 123 306 1455
Cuts at: 4120 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 306 228 221 217
123
BanII G_rGCy'C
Cuts at: 1391 2553 2663 1391
Size: 1162 110 4072
Fragments arranged by size:
4072 1162 110
BbeI G_GCGC'C
Cuts at: 2012 2240 2363 2012
Size: 228 123 4993
Fragments arranged by size:
4993 228 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4373
BbvCI CC'TCA_GC
Cuts at: 2606 2606
Size: 5344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3535 3569 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4446 3236
Size: 1210 4134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2325 2368 2589
Size: 107 122 71 631 605 653 43 221
Cuts at: 2589 3955 136
Size: 1366 1525
Fragments arranged by size:
1525 1366 653 631 605 221 122 107
71 43
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 3405 4561 1790
Size: 1615 1156 2573
Fragments arranged by size:
2573 1615 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4033 442
Size: 416 53 83 399 24 1658 958 1753
Fragments arranged by size:
1753 1658 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4004 1239
Size: 2765 2579
Bpu10I CC'TnA_GC
Cuts at: 2547 2606 2547
Size: 59 5285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 3469 4334 4632 2360
Size: 1109 865 298 3072
Fragments arranged by size:
3072 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 4007 882
Size: 617 1023 1485 2219
Fragments arranged by size:
2219 1485 1023 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 2512 3135 493
Size: 711 1068 240 623 2702
Fragments arranged by size:
2702 1068 711 623 240
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2088 2237 2360
Size: 53 83 186 936 476 79 149 123
Cuts at: 2360 3151 3533 4168 275
Size: 791 382 635 1451
Fragments arranged by size:
1451 936 791 635 476 382 186 149
123 83 79 53
BsaWI w'CCGG_w
Cuts at: 3777 4175 4449 4596 5007 3777
Size: 398 274 147 411 4114
Fragments arranged by size:
4114 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5119
Fragments arranged by size:
5119 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5344
BseYI C'CCAG_C
Cuts at: 2337 4547 5043 2337
Size: 2210 496 2638
Fragments arranged by size:
2638 2210 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4771
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 2519 2794 3555 4583 1540
Size: 275 186 252 266 275 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 275 266 252 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2625 3405 3490 4561 2268
Size: 357 780 85 1071 3051
Fragments arranged by size:
3051 1071 780 357 85
BsmI GAATG_Cn'
Cuts at: 2855 2948 2855
Size: 93 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2553 2625 2663 3405 3490 4561
Size: 399 478 285 72 38 742 85 1071
Cuts at: 4561 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 478 399 285 85 72
38
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 3233 4963 3233
Size: 1730 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 844
Size: 1855 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2374 3231 1394
Size: 429 551 857 3507
Fragments arranged by size:
3507 857 551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2349 2484 3988 4175 1700
Size: 110 438 101 135 1504 187 2869
Fragments arranged by size:
2869 1504 438 187 135 110 101
BsrGI T'GTAC_A
Cuts at: 96 2277 96
Size: 2181 3163
BssHII G'CGCG_C
Cuts at: 1925 2075 2077 1925
Size: 150 2 5192
Fragments arranged by size:
5192 150 2
BssSI C'ACGA_G
Cuts at: 2441 2572 3404 4416 2441
Size: 131 832 1012 3369
Fragments arranged by size:
3369 1012 832 131
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2272 794
Size: 1478 3866
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5344
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2479
Size: 5344
BstYI r'GATC_y
Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339
Size: 702 367 385 17 1452 86 12 346
Cuts at: 5339 1972
Size: 1977
Fragments arranged by size:
1977 1452 702 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3502
Fragments arranged by size:
3502 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4605
Fragments arranged by size:
4605 683 56
BtsI GCAGTG_nn'
Cuts at: 2860 3654 3674 2860
Size: 794 20 4530
Fragments arranged by size:
4530 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2648 2648
Size: 5344
DraIII CAC_nnn'GTG
Cuts at: 2272 2272
Size: 5344
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2437 2516
Size: 54 1425 50 275 18 420 187 79
Cuts at: 2516 2791 3680 5309 8
Size: 275 889 1629 43
Fragments arranged by size:
1629 1425 889 420 275 275 187 79
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 2516 2791 1537
Size: 275 438 266 275 4090
Fragments arranged by size:
4090 438 275 275 266
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 2606 3274 1353
Size: 1052 201 668 3423
Fragments arranged by size:
3423 1052 668 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3931 4305 4451 387
Size: 1064 138 2342 374 146 1280
Fragments arranged by size:
2342 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 3407 4004 4791 5059 1239
Size: 393 890 885 597 787 268 1524
Fragments arranged by size:
1524 890 885 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 2537
Size: 5344
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 2012 2023 2240 2338 2363 4491 1678
Size: 334 11 217 98 25 2128 2531
Fragments arranged by size:
2531 2128 334 217 98 25 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373
Size: 32 135 32 2413 32 42 32 2626
Fragments arranged by size:
2626 2413 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 2688 2934 669
Size: 988 281 349 401 246 3079
Fragments arranged by size:
3079 988 401 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KasI G'GCGC_C
Cuts at: 2008 2236 2359 2008
Size: 228 123 4993
Fragments arranged by size:
4993 228 123
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4458 4642 1305
Size: 3153 184 2007
Fragments arranged by size:
3153 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2618 3302 3661 3820 518
Size: 648 1452 684 359 159 2042
Fragments arranged by size:
2042 1452 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2035 2056 2242 2357 2760 3437 4585
Size: 420 9 21 186 115 403 677 1148
Cuts at: 4585 4830 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 420 403 245 186 115
21 9
NaeI GCC'GGC
Cuts at: 2250 2250
Size: 5344
NarI GG'CG_CC
Cuts at: 2009 2237 2360 2009
Size: 228 123 4993
Fragments arranged by size:
4993 228 123
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3853
Fragments arranged by size:
3853 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NgoMIV G'CCGG_C
Cuts at: 2248 2248
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2702 4247 1132
Size: 97 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 97
PasI CC'CwG_GG
Cuts at: 1348 2416 1348
Size: 1068 4276
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5344
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2696 830
Size: 1866 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
RsrII CG'GwC_CG
Cuts at: 2107 2107
Size: 5344
SacII CC_GC'GG
Cuts at: 2358 2358
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SapI GCTCTTCn'nnn_
Cuts at: 2405 2405
Size: 5344
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2692 3827 4508 4699 826
Size: 219 1647 1135 681 191 1471
Fragments arranged by size:
1647 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2238 2361 2010
Size: 228 123 4993
Fragments arranged by size:
4993 228 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2365 2586 2671 1872
Size: 493 221 85 4545
Fragments arranged by size:
4545 493 221 85
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2375 3448 4349 4611 820
Size: 197 926 432 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 432 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SrfI GCCC'GGGC
Cuts at: 2586 2586
Size: 5344
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5344
StyI C'CwwG_G
Cuts at: 513 1065 2004 2783 513
Size: 552 939 779 3074
Fragments arranged by size:
3074 939 779 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3371 3388 3541 3726 4171 325
Size: 1255 1791 17 153 185 445 1498
Fragments arranged by size:
1791 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 3590 5302
Size: 274 80 33 51 1443 300 1313 1712
Cuts at: 5302 96
Size: 138
Fragments arranged by size:
1712 1443 1313 300 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5304 5336 5304
Size: 32 5312
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2363 2584 2669 1870
Size: 493 221 85 4545
Fragments arranged by size:
4545 493 221 85
XmnI GAAnn'nnTTC
Cuts at: 3473 3473
Size: 5344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII SacI SanDI SexAI SfiI
SwaI XcmI