Restriction map for
pFN21AB7405
(Circular) (Six-base) MAPSORT of: pFN21AB7405.seq Check: 5995 from: 1 to: 6160
pFN21AB7405 6160 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2633 2633
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 2724 2825 4223 5607 5875 1632
Size: 559 533 101 1398 1384 268 1917
Fragments arranged by size:
1917 1398 1384 559 533 268 101
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 3300 1166
Size: 2134 4026
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AlwNI CAG_nnn'CTG
Cuts at: 2476 3903 5475 2476
Size: 1427 1572 3161
Fragments arranged by size:
3161 1572 1427
ApaI G_GGCC'C
Cuts at: 3309 3309
Size: 6160
ApaLI G'TGCA_C
Cuts at: 3008 4217 5373 3008
Size: 1209 1156 3795
Fragments arranged by size:
3795 1209 1156
ApoI r'AATT_y
Cuts at: 1493 3099 3469 3695 3847 5956 1493
Size: 1606 370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1606 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2324 2342 3254 3485 1870
Size: 73 256 125 18 912 231 4545
Fragments arranged by size:
4545 912 256 231 125 73 18
BamHI G'GATC_C
Cuts at: 3490 3857 3490
Size: 367 5793
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2190 3481 4936 618
Size: 325 217 627 403 1291 1455 1842
Fragments arranged by size:
1842 1455 1291 627 403 325 217
BanII G_rGCy'C
Cuts at: 1391 3309 3479 1391
Size: 1918 170 4072
Fragments arranged by size:
4072 1918 170
BbeI G_GCGC'C
Cuts at: 2194 2194
Size: 6160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2257 2682 928
Size: 971 358 425 4406
Fragments arranged by size:
4406 971 425 358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 4351 4385 1903
Size: 34 152 34 2228 34 3678
Fragments arranged by size:
3678 2228 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3072 4052 5262 3072
Size: 980 1210 3970
Fragments arranged by size:
3970 1210 980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4771 136
Size: 107 122 71 631 605 3099 1525
Fragments arranged by size:
3099 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 2252 3234 3291 3577 2252
Size: 982 57 286 4835
Fragments arranged by size:
4835 982 286 57
Bme1580I G_kGCm'C
Cuts at: 1790 3012 3262 3309 4221 5377 1790
Size: 1222 250 47 912 1156 2573
Fragments arranged by size:
2573 1222 1156 912 250 47
BmgBI CAC'GTC
Cuts at: 1318 2028 3277 1318
Size: 710 1249 4201
Fragments arranged by size:
4201 1249 710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3891 4849 442
Size: 416 53 83 399 24 2474 958 1753
Fragments arranged by size:
2474 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3197 4820 1239
Size: 1958 1623 2579
Fragments arranged by size:
2579 1958 1623
Bpu10I CC'TnA_GC
Cuts at: 2168 2168
Size: 6160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4285 5150 5448 4285
Size: 865 298 4997
Fragments arranged by size:
4997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2089 2900 4823 882
Size: 617 590 811 1923 2219
Fragments arranged by size:
2219 1923 811 617 590
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2191 2364 3967 4349
Size: 53 83 186 936 658 173 1603 382
Cuts at: 4349 4984 275
Size: 635 1451
Fragments arranged by size:
1603 1451 936 658 635 382 186 173
83 53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 1178
Size: 30 165 30 720 30 5185
Fragments arranged by size:
5185 720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6160
BseYI C'CCAG_C
Cuts at: 2846 5363 5859 2846
Size: 2517 496 3147
Fragments arranged by size:
3147 2517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3035 1163
Size: 573 1299 4288
Fragments arranged by size:
4288 1299 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2574 2625 3610 4371 5399 1540
Size: 275 186 573 51 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 573 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 2055 2355 3012 3297 4221 4306 5377 2055
Size: 300 657 285 924 85 1071 2838
Fragments arranged by size:
2838 1071 924 657 300 285 85
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
BsmBI CGTCTCn'nnnn_
Cuts at: 3342 3342
Size: 6160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2355 3012 3262 3297 3309 3479
Size: 399 265 300 657 250 35 12 170
Cuts at: 3479 4221 4306 5377 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 399 300 265 250
170 85 35 12
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2633 3515 844
Size: 1789 882 3489
Fragments arranged by size:
3489 1789 882
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 2174 4804 4991 1700
Size: 110 221 143 2630 187 2869
Fragments arranged by size:
2869 2630 221 187 143 110
BsrGI T'GTAC_A
Cuts at: 96 2998 96
Size: 2902 3258
BssHII G'CGCG_C
Cuts at: 1925 2247 2536 1925
Size: 322 289 5549
Fragments arranged by size:
5549 322 289
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2028 794
Size: 1234 4926
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 3490 3857 4242 4259 5711 5797 5809 6155
Size: 1518 367 385 17 1452 86 12 346
Cuts at: 6155 1972
Size: 1977
Fragments arranged by size:
1977 1518 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2514 3066 2514
Size: 552 5608
BtgI C'CryG_G
Cuts at: 513 1065 2004 2531 2760 3400 513
Size: 552 939 527 229 640 3273
Fragments arranged by size:
3273 939 640 552 527 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2092 2167 487
Size: 683 56 866 75 4480
Fragments arranged by size:
4480 866 683 75 56
BtsI GCAGTG_nn'
Cuts at: 2837 3001 3260 3676 4470 4490 2837
Size: 164 259 416 794 20 4507
Fragments arranged by size:
4507 794 416 259 164 20
ClaI AT'CG_AT
Cuts at: 1554 2334 1554
Size: 780 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3342 3377 529
Size: 35 2778 35 3312
Fragments arranged by size:
3312 2778 35 35
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DraIII CAC_nnn'GTG
Cuts at: 3136 3136
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2778 3607
Size: 54 1425 50 275 18 741 207 829
Cuts at: 3607 4496 6125 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 829 741 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2571 3607 1537
Size: 275 759 1036 4090
Fragments arranged by size:
4090 1036 759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2801 2949 3340 4090 1353
Size: 692 756 148 391 750 3423
Fragments arranged by size:
3423 756 750 692 391 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 2495 2558 4747 5121 5267
Size: 1064 138 501 405 63 2189 374 146
Cuts at: 5267 387
Size: 1280
Fragments arranged by size:
2189 1280 1064 501 405 374 146 138
63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2724 2825 3197 4223 4820 5607
Size: 393 559 533 101 372 1026 597 787
Cuts at: 5607 5875 1239
Size: 268 1524
Fragments arranged by size:
1524 1026 787 597 559 533 393 372
268 101
EcoO109I rG'GnC_Cy
Cuts at: 2510 2527 2589 2815 3306 2510
Size: 17 62 226 491 5364
Fragments arranged by size:
5364 491 226 62 17
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
EcoRV GAT'ATC
Cuts at: 3080 3080
Size: 6160
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2175 2194 2214 2502 5307 1678
Size: 497 19 20 288 2805 2531
Fragments arranged by size:
2805 2531 497 288 20 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2684 2716 4801
Size: 32 135 32 563 32 517 32 2085
Cuts at: 4801 4833 4875 4907 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2085 563 517 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 2947 3445 3504 3750 669
Size: 988 281 445 564 498 59 246 3079
Fragments arranged by size:
3079 988 564 498 445 281 246 59
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KasI G'GCGC_C
Cuts at: 2190 2190
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2166 5274 5458 1305
Size: 840 21 3108 184 2007
Fragments arranged by size:
3108 2007 840 184 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4338
Fragments arranged by size:
4338 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 2294 3279 3300 4118 4477 4636
Size: 648 882 246 985 21 818 359 159
Cuts at: 4636 518
Size: 2042
Fragments arranged by size:
2042 985 882 818 648 359 246 159
21
MspA1I CmG'CkG
Cuts at: 1606 2467 2476 2533 2912 3241 3576 4253 5401
Size: 861 9 57 379 329 335 677 1148
Cuts at: 5401 5646 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 861 677 379 335 329 245
57 9
NaeI GCC'GGC
Cuts at: 2033 2176 2033
Size: 143 6017
NarI GG'CG_CC
Cuts at: 2191 2191
Size: 6160
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4669
Fragments arranged by size:
4669 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 2031 2174 2031
Size: 143 6017
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2137 2738 3518 5063 1132
Size: 97 908 601 780 1545 2229
Fragments arranged by size:
2229 1545 908 780 601 97
PasI CC'CwG_GG
Cuts at: 1348 2752 1348
Size: 1404 4756
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 2406 2406
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2589 2815 2527
Size: 62 226 5872
Fragments arranged by size:
5872 226 62
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspOMI G'GGCC_C
Cuts at: 3305 3305
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2463 3512 830
Size: 1633 1049 3478
Fragments arranged by size:
3478 1633 1049
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2476 3241 2476
Size: 765 5395
SacII CC_GC'GG
Cuts at: 2534 2534
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SapI GCTCTTCn'nnn_
Cuts at: 2045 2949 2045
Size: 904 5256
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2459 2924 3508 4643 5324 5515 826
Size: 219 1414 465 584 1135 681 191 1471
Fragments arranged by size:
1471 1414 1135 681 584 465 219 191
SfoI GGC'GCC
Cuts at: 2192 2192
Size: 6160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 2201 3256 3487 1872
Size: 329 1055 231 4545
Fragments arranged by size:
4545 1055 329 231
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4264 5165 5427 820
Size: 197 926 2321 901 262 1553
Fragments arranged by size:
2321 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2004 3222 3599 513
Size: 552 939 1218 377 3074
Fragments arranged by size:
3074 1218 939 552 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2675 4187 4204 4357 4542 4987 325
Size: 1255 1095 1512 17 153 185 445 1498
Fragments arranged by size:
1512 1498 1255 1095 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2725 2998 4406
Size: 274 80 33 51 1443 748 273 1408
Cuts at: 4406 6118 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1408 748 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6120 6152 6120
Size: 32 6128
Tth111I GACn'n_nGTC
Cuts at: 1316 2448 3408 1316
Size: 1132 960 4068
Fragments arranged by size:
4068 1132 960
XbaI T'CTAG_A
Cuts at: 3313 3496 3313
Size: 183 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 2199 3254 3485 1870
Size: 329 1055 231 4545
Fragments arranged by size:
4545 1055 329 231
XmnI GAAnn'nnTTC
Cuts at: 4289 4289
Size: 6160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I EcoICRI EcoNI FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SrfI SwaI XcmI