Restriction map for
pFN21AB7401
(Circular) (Six-base) MAPSORT of: pFN21AB7401.seq Check: 2891 from: 1 to: 5296
pFN21AB7401 5296 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3106 278
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 1937
Size: 702 4594
Acc65I G'GTAC_C
Cuts at: 2617 2617
Size: 5296
AclI AA'CG_TT
Cuts at: 1563 3423 3796 1563
Size: 1860 373 3063
Fragments arranged by size:
3063 1860 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 3359 4743 5011 1632
Size: 574 1153 1384 268 1917
Fragments arranged by size:
1917 1384 1153 574 268
AfeI AGC'GCT
Cuts at: 2180 2180
Size: 5296
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5099
AflIII A'CryG_T
Cuts at: 1203 4195 5172 1203
Size: 2992 977 1327
Fragments arranged by size:
2992 1327 977
AgeI A'CCGG_T
Cuts at: 4127 4127
Size: 5296
AhdI GACnn_n'nnGTC
Cuts at: 4025 4025
Size: 5296
AleI CACnn'nnGTG
Cuts at: 1166 2384 2448 1166
Size: 1218 64 4014
Fragments arranged by size:
4014 1218 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5296
AlwNI CAG_nnn'CTG
Cuts at: 3039 4611 3039
Size: 1572 3724
ApaI G_GGCC'C
Cuts at: 2204 2204
Size: 5296
ApaLI G'TGCA_C
Cuts at: 3353 4509 3353
Size: 1156 4140
ApoI r'AATT_y
Cuts at: 1493 2605 2831 2983 5092 1493
Size: 1112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3850 160
Size: 872 2818 1606
Fragments arranged by size:
2818 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 1870
Size: 73 678 4545
Fragments arranged by size:
4545 678 73
BamHI G'GATC_C
Cuts at: 2626 2993 2626
Size: 367 4929
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2432 2617 4072 618
Size: 325 217 627 645 185 1455 1842
Fragments arranged by size:
1842 1455 645 627 325 217 185
BanII G_rGCy'C
Cuts at: 1391 2204 2312 2460 2537 2615 1391
Size: 813 108 148 77 78 4072
Fragments arranged by size:
4072 813 148 108 78 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 2359 928
Size: 971 451 9 3865
Fragments arranged by size:
3865 971 451 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2495 2529 3487 3521 1903
Size: 34 558 34 958 34 3678
Fragments arranged by size:
3678 958 558 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4398 3188
Size: 1210 4086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3907 136
Size: 107 122 71 631 605 2235 1525
Fragments arranged by size:
2235 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5291 5291
Size: 5296
BlpI GC'TnA_GC
Cuts at: 2147 2713 2147
Size: 566 4730
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2435 3357 4513 1790
Size: 414 231 922 1156 2573
Fragments arranged by size:
2573 1156 922 414 231
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3027 3985 442
Size: 416 53 83 399 24 1610 958 1753
Fragments arranged by size:
1753 1610 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 3956 1239
Size: 1205 1512 2579
Fragments arranged by size:
2579 1512 1205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3421 4286 4584 3421
Size: 865 298 4133
Fragments arranged by size:
4133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3959 882
Size: 617 2460 2219
Fragments arranged by size:
2460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 493
Size: 711 1883 2702
Fragments arranged by size:
2702 1883 711
BsaBI GATnn'nnATC
Cuts at: 5290 5290
Size: 5296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3103 3485 4120 275
Size: 53 83 186 936 1570 382 635 1451
Fragments arranged by size:
1570 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3729 4127 4401 4548 4959 3729
Size: 398 274 147 411 4066
Fragments arranged by size:
4066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 1178
Size: 30 165 30 891 30 4150
Fragments arranged by size:
4150 891 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5296
BseYI C'CCAG_C
Cuts at: 2479 2563 4499 4995 2479
Size: 84 1936 496 2780
Fragments arranged by size:
2780 1936 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2210 1163
Size: 573 450 24 4249
Fragments arranged by size:
4249 573 450 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 2211 2746 3507 4535 1540
Size: 275 186 115 95 535 761 1028 2301
Fragments arranged by size:
2301 1028 761 535 275 186 115 95
BsiHKAI G_wGCw'C
Cuts at: 2460 3357 3442 4513 2460
Size: 897 85 1071 3243
Fragments arranged by size:
3243 1071 897 85
BsmI GAATG_Cn'
Cuts at: 2807 2900 2807
Size: 93 5203
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2312 2435 2460 2537 2615 3357
Size: 399 414 108 123 25 77 78 742
Cuts at: 3357 3442 4513 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 123 108 85
78 77 25
BspEI T'CCGG_A
Cuts at: 4959 4959
Size: 5296
BspHI T'CATG_A
Cuts at: 3185 4915 3185
Size: 1730 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2651 844
Size: 1807 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3183 1394
Size: 429 1360 3507
Fragments arranged by size:
3507 1360 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 2565 3791 3965 65
Size: 1183 1122 195 1226 174 1396
Fragments arranged by size:
1396 1226 1183 1122 195 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2206 3940 4127 1700
Size: 110 396 1734 187 2869
Fragments arranged by size:
2869 1734 396 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5296
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5296
BssSI C'ACGA_G
Cuts at: 2303 3356 4368 2303
Size: 1053 1012 3231
Fragments arranged by size:
3231 1053 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5296
BstBI TT'CG_AA
Cuts at: 4100 4100
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5296
BstYI r'GATC_y
Cuts at: 1972 2626 2993 3378 3395 4847 4933 4945 5291
Size: 654 367 385 17 1452 86 12 346
Cuts at: 5291 1972
Size: 1977
Fragments arranged by size:
1977 1452 654 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2110 513
Size: 552 939 106 3699
Fragments arranged by size:
3699 939 552 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4557
Fragments arranged by size:
4557 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3606 3626 2812
Size: 794 20 4482
Fragments arranged by size:
4482 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5261
DraI TTT'AAA
Cuts at: 2600 2600
Size: 5296
DraIII CAC_nnn'GTG
Cuts at: 2406 2406
Size: 5296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4303 809
Size: 507 2987 1802
Fragments arranged by size:
2987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2113 2208
Size: 54 1425 50 275 18 268 15 95
Cuts at: 2208 2256 2743 3632 5261 8
Size: 48 487 889 1629 43
Fragments arranged by size:
1629 1425 889 487 275 268 95 54
50 48 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2113 2208 2743 1537
Size: 275 301 95 535 4090
Fragments arranged by size:
4090 535 301 275 95
EarI CTCTTCn'nnn_
Cuts at: 1353 2132 2288 2448 3226 1353
Size: 779 156 160 778 3423
Fragments arranged by size:
3423 779 778 160 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2311 2485 3883 4257 4403 387
Size: 1064 138 722 174 1398 374 146 1280
Fragments arranged by size:
1398 1280 1064 722 374 174 146 138
EcoICRI GAG'CTC
Cuts at: 2458 2458
Size: 5296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2444 3359 3956 4743 5011 1239
Size: 393 574 238 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 597 574 393 268 238
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2294
Size: 5296
EcoO109I rG'GnC_Cy
Cuts at: 2200 2200
Size: 5296
EcoRI G'AATT_C
Cuts at: 2605 2983 2605
Size: 378 4918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2570 2570
Size: 5296
FspI TGC'GCA
Cuts at: 3802 3802
Size: 5296
HaeII r_GCGC'y
Cuts at: 1678 2012 2154 2182 2349 4443 1678
Size: 334 142 28 167 2094 2531
Fragments arranged by size:
2531 2094 334 167 142 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3937 3969 4011 4043 1373
Size: 32 135 32 2365 32 42 32 2626
Fragments arranged by size:
2626 2365 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2640 2886 669
Size: 988 281 702 246 3079
Fragments arranged by size:
3079 988 702 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5296
HpaI GTT'AAC
Cuts at: 2886 2886
Size: 5296
KpnI G_GTAC'C
Cuts at: 2621 2621
Size: 5296
MfeI C'AATT_G
Cuts at: 2517 2895 2517
Size: 378 4918
MluI A'CGCG_T
Cuts at: 5172 5172
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4410 4594 1305
Size: 3105 184 2007
Fragments arranged by size:
3105 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3474
Fragments arranged by size:
3474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2448 3254 3613 3772 518
Size: 648 1218 64 806 359 159 2042
Fragments arranged by size:
2042 1218 806 648 359 159 64
MspA1I CmG'CkG
Cuts at: 1606 2086 2112 2118 2448 2712 3389 4537 4782
Size: 480 26 6 330 264 677 1148 245
Cuts at: 4782 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 480 330 264 245 26
6
NaeI GCC'GGC
Cuts at: 2208 2208
Size: 5296
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3805
Fragments arranged by size:
3805 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5296
NgoMIV G'CCGG_C
Cuts at: 2206 2206
Size: 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5296
NotI GC'GGCC_GC
Cuts at: 2113 2743 2113
Size: 630 4666
NspI r_CATG'y
Cuts at: 1132 1229 2143 2654 4199 1132
Size: 97 914 511 1545 2229
Fragments arranged by size:
2229 1545 914 511 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5296
PciI A'CATG_T
Cuts at: 4195 4195
Size: 5296
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5296
PfoI T'CCnGG_A
Cuts at: 2460 2460
Size: 5296
PmeI GTTT'AAAC
Cuts at: 2600 2600
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4878 4910 3365
Size: 32 1481 32 3751
Fragments arranged by size:
3751 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5296
PsiI TTA'TAA
Cuts at: 2866 2866
Size: 5296
PspOMI G'GGCC_C
Cuts at: 2200 2200
Size: 5296
PstI C_TGCA'G
Cuts at: 830 2648 830
Size: 1818 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5296
PvuII CAG'CTG
Cuts at: 2448 2448
Size: 5296
SacI G_AGCT'C
Cuts at: 2460 2460
Size: 5296
SacII CC_GC'GG
Cuts at: 2113 2113
Size: 5296
SalI G'TCGA_C
Cuts at: 1936 2638 1936
Size: 702 4594
SapI GCTCTTCn'nnn_
Cuts at: 2132 2288 2448 2132
Size: 156 160 4980
Fragments arranged by size:
4980 160 156
SbfI CC_TGCA'GG
Cuts at: 2648 2648
Size: 5296
ScaI AGT'ACT
Cuts at: 3544 5256 3544
Size: 1712 3584
SfcI C'TryA_G
Cuts at: 826 1045 2644 3779 4460 4651 826
Size: 219 1599 1135 681 191 1471
Fragments arranged by size:
1599 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5296
SmaI CCC'GGG
Cuts at: 1872 2623 1872
Size: 751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3400 4301 4563 820
Size: 197 926 1457 901 262 1553
Fragments arranged by size:
1553 1457 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3087 493
Size: 2594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5296
SphI G_CATG'C
Cuts at: 1132 2654 1132
Size: 1522 3774
SspI AAT'ATT
Cuts at: 5 52 3220 5
Size: 47 3168 2081
Fragments arranged by size:
3168 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5296
StyI C'CwwG_G
Cuts at: 513 1065 2004 2735 513
Size: 552 939 731 3074
Fragments arranged by size:
3074 939 731 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3323 3340 3493 3678 4123 325
Size: 1255 1743 17 153 185 445 1498
Fragments arranged by size:
1743 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3542 5254 96
Size: 274 80 33 51 1443 1565 1712 138
Fragments arranged by size:
1712 1565 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2294 2326 5256 5288 2294
Size: 32 2930 32 2302
Fragments arranged by size:
2930 2302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5296
XbaI T'CTAG_A
Cuts at: 2632 2632
Size: 5296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5296
XmaI C'CCGG_G
Cuts at: 1870 2621 1870
Size: 751 4545
XmnI GAAnn'nnTTC
Cuts at: 3425 3425
Size: 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3104 276
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI