Restriction map for
pFN21AB7398
(Circular) (Six-base) MAPSORT of: pFN21AB7398.seq Check: 3131 from: 1 to: 5680
pFN21AB7398 5680 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2278 2278
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 2633 2744 3001 2633
Size: 111 257 5312
Fragments arranged by size:
5312 257 111
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 2421 3743 5127 5395 1632
Size: 623 166 1322 1384 268 1917
Fragments arranged by size:
1917 1384 1322 623 268 166
AfeI AGC'GCT
Cuts at: 2394 2394
Size: 5680
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 2206 2524 4409 2206
Size: 318 1885 3477
Fragments arranged by size:
3477 1885 318
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2044 1184
Size: 860 4820
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2763 2796 2829 4234 160
Size: 872 1731 33 33 1405 1606
Fragments arranged by size:
1731 1606 1405 872 33 33
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 3005 1870
Size: 73 1062 4545
Fragments arranged by size:
4545 1062 73
AvrII C'CTAG_G
Cuts at: 2204 2963 2204
Size: 759 4921
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2860 2893 2860
Size: 33 5647
BamHI G'GATC_C
Cuts at: 2344 3010 3377 2344
Size: 666 367 4647
Fragments arranged by size:
4647 666 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2633 2744 3001 4456 618
Size: 325 217 627 846 111 257 1455 1842
Fragments arranged by size:
1842 1455 846 627 325 257 217 111
BanII G_rGCy'C
Cuts at: 1391 2999 1391
Size: 1608 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2051 2460 928
Size: 971 152 409 4148
Fragments arranged by size:
4148 971 409 152
BbvCI CC'TCA_GC
Cuts at: 2139 2373 2139
Size: 234 5446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2680 2714 3871 3905 1903
Size: 34 50 34 659 34 1157 34 3678
Fragments arranged by size:
3678 1157 659 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2781 2814 2847 3572 4782 2781
Size: 33 33 725 1210 3679
Fragments arranged by size:
3679 1210 725 33 33
BclI T'GATC_A
Cuts at: 1548 2620 1548
Size: 1072 4608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2916 4291 136
Size: 107 122 71 631 605 1244 1375 1525
Fragments arranged by size:
1525 1375 1244 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 2240 3097 2240
Size: 857 4823
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4897 1790
Size: 1951 1156 2573
Fragments arranged by size:
2573 1951 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2564 2640 3411
Size: 416 53 83 399 24 1147 76 771
Cuts at: 3411 4369 442
Size: 958 1753
Fragments arranged by size:
1753 1147 958 771 416 399 83 76
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2316 2049
Size: 267 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2404 2919 4340 1239
Size: 1165 515 1421 2579
Fragments arranged by size:
2579 1421 1165 515
Bpu10I CC'TnA_GC
Cuts at: 2139 2373 2139
Size: 234 5446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2601 4343 882
Size: 617 1102 1742 2219
Fragments arranged by size:
2219 1742 1102 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2077 2107 1178
Size: 30 165 30 674 30 4751
Fragments arranged by size:
4751 674 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2025 2117 2498 1186
Size: 839 92 381 4368
Fragments arranged by size:
4368 839 381 92
BseYI C'CCAG_C
Cuts at: 2751 2784 2817 2850 2935 4883 5379 2751
Size: 33 33 33 85 1948 496 3052
Fragments arranged by size:
3052 1948 496 85 33 33 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 1163
Size: 573 573 4534
Fragments arranged by size:
4534 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2999 3741 3826 4897 1391
Size: 399 1209 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2278 3035 844
Size: 1434 757 3489
Fragments arranged by size:
3489 1434 757
BsrBI CCG'CTC
Cuts at: 1394 1823 2195 3567 1394
Size: 429 372 1372 3507
Fragments arranged by size:
3507 1372 429 372
BsrDI GCAATG_nn'
Cuts at: 65 1248 4175 4349 65
Size: 1183 2927 174 1396
Fragments arranged by size:
2927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2344 3010 3377 3762 3779 5231 5317 5329
Size: 372 666 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 666 385 372 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2340 2709 2340
Size: 369 5311
BtgI C'CryG_G
Cuts at: 513 1065 2322 513
Size: 552 1257 3871
Fragments arranged by size:
3871 1257 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2452 2978 3196 3990 4010 2292
Size: 160 526 218 794 20 3962
Fragments arranged by size:
3962 794 526 218 160 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645
Size: 54 1425 50 275 18 1297 889 1629
Cuts at: 5645 8
Size: 43
Fragments arranged by size:
1629 1425 1297 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2926 3610 1353
Size: 1573 684 3423
Fragments arranged by size:
3423 1573 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2897 4267 4641 4787 387
Size: 1064 138 1308 1370 374 146 1280
Fragments arranged by size:
1370 1308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2404 2421 2919 3743 4340 5127
Size: 393 623 149 17 498 824 597 787
Cuts at: 5127 5395 1239
Size: 268 1524
Fragments arranged by size:
1524 824 787 623 597 498 393 268
149 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2274 2513 2274
Size: 239 5441
EcoO109I rG'GnC_Cy
Cuts at: 2669 2669
Size: 5680
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2396 4827 1678
Size: 718 2431 2531
Fragments arranged by size:
2531 2431 718
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2510 2542 4321
Size: 32 135 32 620 32 286 32 1779
Cuts at: 4321 4353 4395 4427 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1779 620 286 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 2637 2748 3005 2637
Size: 111 257 5312
Fragments arranged by size:
5312 257 111
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3638 3997 4156 518
Size: 648 2472 359 159 2042
Fragments arranged by size:
2472 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2751 2784 2817 2850 2939 3096 3773 4921
Size: 1145 33 33 33 89 157 677 1148
Cuts at: 4921 5166 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1145 677 245 157 89 33
33 33
NcoI C'CATG_G
Cuts at: 513 1065 2322 513
Size: 552 1257 3871
Fragments arranged by size:
3871 1257 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2628 2672 1348
Size: 1280 44 4356
Fragments arranged by size:
4356 1280 44
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2122 1108
Size: 1014 4666
PfoI T'CCnGG_A
Cuts at: 2773 2806 2839 2896 2773
Size: 33 33 57 5557
Fragments arranged by size:
5557 57 33 33
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2275 2287 3032 830
Size: 1445 12 745 3478
Fragments arranged by size:
3478 1445 745 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2751 2784 2817 2850 2939 2751
Size: 33 33 33 89 5492
Fragments arranged by size:
5492 89 33 33 33
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2926 2926
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 2275 3032 2275
Size: 757 4923
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2271 2283 3028 4163 4844 5035 826
Size: 219 1226 12 745 1135 681 191 1471
Fragments arranged by size:
1471 1226 1135 745 681 219 191 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 2761 2794 2827 2860 3604 5
Size: 47 2709 33 33 33 744 2081
Fragments arranged by size:
2709 2081 744 47 33 33 33
StuI AGG'CCT
Cuts at: 1517 2073 2487 1517
Size: 556 414 4710
Fragments arranged by size:
4710 556 414
StyI C'CwwG_G
Cuts at: 513 1065 2204 2322 2348 2496 2963 3119 513
Size: 552 1139 118 26 148 467 156 3074
Fragments arranged by size:
3074 1139 552 467 156 148 118 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2509 3707 3724 3877 4062 4507 325
Size: 1255 929 1198 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1198 929 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2077 2109 5640 5672 2077
Size: 32 3531 32 2085
Fragments arranged by size:
3531 2085 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2748 2781 2814 2847 2748
Size: 33 33 33 5581
Fragments arranged by size:
5581 33 33 33
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI