Restriction map for
pFN21AB7392
(Circular) (Six-base) MAPSORT of: pFN21AB7392.seq Check: 7960 from: 1 to: 5725
pFN21AB7392 5725 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2144 3535 278
Size: 53 83 186 936 608 1391 2468
Fragments arranged by size:
2468 1391 936 608 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 2043 2549 3034 3260 3412 5521 1493
Size: 550 506 485 226 152 2109 1697
Fragments arranged by size:
2109 1697 550 506 485 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2313 2775 3050 1870
Size: 73 237 133 462 275 4545
Fragments arranged by size:
4545 462 275 237 133 73
AvrII C'CTAG_G
Cuts at: 2809 2809
Size: 5725
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2863 3046 4501 618
Size: 325 217 627 1076 183 1455 1842
Fragments arranged by size:
1842 1455 1076 627 325 217 183
BanII G_rGCy'C
Cuts at: 1391 2116 2295 2709 2914 3044 1391
Size: 725 179 414 205 130 4072
Fragments arranged by size:
4072 725 414 205 179 130
BbeI G_GCGC'C
Cuts at: 2867 2867
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2247 2886 928
Size: 971 348 639 3767
Fragments arranged by size:
3767 971 639 348
BbvCI CC'TCA_GC
Cuts at: 2874 2874
Size: 5725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 2100 1548
Size: 552 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 3786 4942 1790
Size: 1996 1156 2573
Fragments arranged by size:
2573 1996 1156
BmgBI CAC'GTC
Cuts at: 1318 2098 1318
Size: 780 4945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2960 4385 1239
Size: 1721 1425 2579
Fragments arranged by size:
2579 1721 1425
Bpu10I CC'TnA_GC
Cuts at: 2874 2874
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 3850 4715 5013 2288
Size: 1562 865 298 3000
Fragments arranged by size:
3000 1562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2864 3532 3914
Size: 53 83 186 936 608 723 668 382
Cuts at: 3914 4549 275
Size: 635 1451
Fragments arranged by size:
1451 936 723 668 635 608 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2665 4158 4556 4830 4977 5388 2665
Size: 1493 398 274 147 411 3002
Fragments arranged by size:
3002 1493 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2958 2988 1178
Size: 30 165 30 1555 30 3915
Fragments arranged by size:
3915 1555 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2222 2225 2228 2231 2303 1186
Size: 1027 9 3 3 3 72 4608
Fragments arranged by size:
4608 1027 72 9 3 3 3
BseYI C'CCAG_C
Cuts at: 4928 5424 4928
Size: 496 5229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2802 1163
Size: 573 1066 4086
Fragments arranged by size:
4086 1066 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 3786 3871 4942 2295
Size: 1491 85 1071 3078
Fragments arranged by size:
3078 1491 1071 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2295 2709 2914 3044 3786 3871
Size: 399 326 179 414 205 130 742 85
Cuts at: 3871 4942 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 326 205 179
130 85
BspEI T'CCGG_A
Cuts at: 2665 5388 2665
Size: 2723 3002
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3080 844
Size: 2236 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 3612 1394
Size: 429 497 1292 3507
Fragments arranged by size:
3507 1292 497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2952 4369 4556 1700
Size: 110 1142 1417 187 2869
Fragments arranged by size:
2869 1417 1142 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2285 3785 4797 2285
Size: 1500 1012 3213
Fragments arranged by size:
3213 1500 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2813 3055 3422 3807 3824 5276 5362 5374
Size: 841 242 367 385 17 1452 86 12
Cuts at: 5374 5720 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 841 385 367 346 242 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2600 513
Size: 552 1535 3638
Fragments arranged by size:
3638 1535 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 3241 4035 4055 3241
Size: 794 20 4911
Fragments arranged by size:
4911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2603 3172 4061
Size: 54 1425 50 275 18 773 569 889
Cuts at: 4061 5690 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 773 569 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2190 2199 2217 2223 2716 2942 3655
Size: 749 88 9 18 6 493 226 713
Cuts at: 3655 1353
Size: 3423
Fragments arranged by size:
3423 749 713 493 226 88 18 9
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2293 2293
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2960 3788 4385 5172 5440 1239
Size: 393 1328 828 597 787 268 1524
Fragments arranged by size:
1524 1328 828 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2043 3034 3412 2043
Size: 991 378 4356
Fragments arranged by size:
4356 991 378
EcoRV GAT'ATC
Cuts at: 2072 2072
Size: 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2063 2063
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2867 2874 4872 1678
Size: 1189 7 1998 2531
Fragments arranged by size:
2531 1998 1189 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2771 2803 3005
Size: 32 135 32 911 32 256 32 202
Cuts at: 3005 3037 4366 4398 4440 4472 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 911 256 202 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2863 2863
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2587 4839 5023 1305
Size: 1282 2252 184 2007
Fragments arranged by size:
2252 2007 1282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3903
Fragments arranged by size:
3903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 3683 4042 4201 518
Size: 648 1260 1257 359 159 2042
Fragments arranged by size:
2042 1260 1257 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2641 3141 3818 4966 5211 1606
Size: 486 549 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 549 500 486 245
NarI GG'CG_CC
Cuts at: 2864 2864
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 3083 4628 1132
Size: 97 1854 1545 2229
Fragments arranged by size:
2229 1854 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2027 2251 1108
Size: 919 224 4582
Fragments arranged by size:
4582 919 224
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
SacI G_AGCT'C
Cuts at: 2295 2295
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SapI GCTCTTCn'nnn_
Cuts at: 2942 2942
Size: 5725
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 2620 3973 5685 2620
Size: 1353 1712 2660
Fragments arranged by size:
2660 1712 1353
SfcI C'TryA_G
Cuts at: 826 1045 3073 4208 4889 5080 826
Size: 219 2028 1135 681 191 1471
Fragments arranged by size:
2028 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2865 2865
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 2315 3052 1872
Size: 443 737 4545
Fragments arranged by size:
4545 737 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 3829 4730 4992 820
Size: 197 926 360 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 360 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 2470 3649 5
Size: 47 2418 1179 2081
Fragments arranged by size:
2418 2081 1179 47
StuI AGG'CCT
Cuts at: 1517 2760 1517
Size: 1243 4482
StyI C'CwwG_G
Cuts at: 513 1065 2809 3164 513
Size: 552 1744 355 3074
Fragments arranged by size:
3074 1744 552 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2836 3752 3769 3922 4107 4552 325
Size: 1255 1256 916 17 153 185 445 1498
Fragments arranged by size:
1498 1256 1255 916 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2618 3971 5683
Size: 274 80 33 51 1443 641 1353 1712
Cuts at: 5683 96
Size: 138
Fragments arranged by size:
1712 1443 1353 641 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 2330 2380 3061 2330
Size: 50 681 4994
Fragments arranged by size:
4994 681 50
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 2313 3050 1870
Size: 443 737 4545
Fragments arranged by size:
4545 737 443
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2142 3533 276
Size: 53 83 186 936 608 1391 2468
Fragments arranged by size:
2468 1391 936 608 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI