Restriction map for
pFN21AB7390
(Circular) (Six-base) MAPSORT of: pFN21AB7390.seq Check: 3681 from: 1 to: 5668
pFN21AB7390 5668 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2123 2123
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2218 2515 2761 3731 5115 5383 1632
Size: 586 297 246 970 1384 268 1917
Fragments arranged by size:
1917 1384 970 586 297 268 246
AfeI AGC'GCT
Cuts at: 2372 2372
Size: 5668
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 2460 4397 2460
Size: 1937 3731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2272 2770 3411 4983 2272
Size: 498 641 1572 2957
Fragments arranged by size:
2957 1572 641 498
ApaLI G'TGCA_C
Cuts at: 2072 3725 4881 2072
Size: 1653 1156 2859
Fragments arranged by size:
2859 1653 1156
ApoI r'AATT_y
Cuts at: 1493 2510 2977 3203 3355 5464 1493
Size: 1017 467 226 152 2109 1697
Fragments arranged by size:
2109 1697 1017 467 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 2915 2993 1870
Size: 73 813 159 78 4545
Fragments arranged by size:
4545 813 159 78 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2421 2948 2989 4444 618
Size: 325 217 627 634 527 41 1455 1842
Fragments arranged by size:
1842 1455 634 627 527 325 217 41
BanII G_rGCy'C
Cuts at: 1391 2099 2630 2916 2987 1391
Size: 708 531 286 71 4072
Fragments arranged by size:
4072 708 531 286 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2656 4279 136
Size: 107 122 71 631 605 984 1623 1525
Fragments arranged by size:
1623 1525 984 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2093 2252 3085 2093
Size: 159 833 4676
Fragments arranged by size:
4676 833 159
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2951 3729 4885 1790
Size: 286 875 778 1156 2573
Fragments arranged by size:
2573 1156 875 778 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2573 3399 4357
Size: 416 53 83 399 24 1156 826 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1156 958 826 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
Bpu10I CC'TnA_GC
Cuts at: 2342 2342
Size: 5668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2936 3475 3857 4492
Size: 53 83 186 936 1403 539 382 635
Cuts at: 4492 275
Size: 1451
Fragments arranged by size:
1451 1403 936 635 539 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2448 2478 1178
Size: 30 165 30 1045 30 4368
Fragments arranged by size:
4368 1045 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2638 2785 2866 2924 1186
Size: 1027 425 147 81 58 3930
Fragments arranged by size:
3930 1027 425 147 81 58
BseYI C'CCAG_C
Cuts at: 2158 2265 2367 2631 4871 5367 2158
Size: 107 102 264 2240 496 2459
Fragments arranged by size:
2459 2240 496 264 107 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2069 2076 2099 2630 2736 2892 3729 3814 4885
Size: 7 23 531 106 156 837 85 1071
Cuts at: 4885 2069
Size: 2852
Fragments arranged by size:
2852 1071 837 531 156 106 85 23
7
BsmI GAATG_Cn'
Cuts at: 2175 3179 3272 2175
Size: 1004 93 4571
Fragments arranged by size:
4571 1004 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2927 2927
Size: 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2076 2099 2630 2736 2892 2916
Size: 399 279 7 23 531 106 156 24
Cuts at: 2916 2951 2987 3729 3814 4885 1391
Size: 35 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 531 399 279 156 106
85 36 35 24 23 7
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2123 2755 3023 844
Size: 1279 632 268 3489
Fragments arranged by size:
3489 1279 632 268
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2412 4312 4499 1700
Size: 110 602 1900 187 2869
Fragments arranged by size:
2869 1900 602 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 2297 3728 4740 2297
Size: 1431 1012 3225
Fragments arranged by size:
3225 1431 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 2167 4472 2167
Size: 2305 3363
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstXI CCAn_nnnn'nTGG
Cuts at: 2878 2959 2878
Size: 81 5587
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2034 2943 513
Size: 552 969 909 3238
Fragments arranged by size:
3238 969 909 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 2642 3184 3978 3998 2032
Size: 610 542 794 20 3702
Fragments arranged by size:
3702 794 610 542 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2038 4675 809
Size: 507 722 2637 1802
Fragments arranged by size:
2637 1802 722 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2355 3115
Size: 54 1425 50 275 18 273 252 760
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 760 275 273 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2231 2237 2303 2393 2491 3598 1353
Size: 878 6 66 90 98 1107 3423
Fragments arranged by size:
3423 1107 878 98 90 66 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2097 2628 2097
Size: 531 5137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2218 2515 2761 3731 4328 5115 5383
Size: 393 586 297 246 970 597 787 268
Cuts at: 5383 1239
Size: 1524
Fragments arranged by size:
1524 970 787 597 586 393 297 268
246
EcoO109I rG'GnC_Cy
Cuts at: 2767 2874 2767
Size: 107 5561
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2374 2817 2903 4815 1678
Size: 696 443 86 1912 2531
Fragments arranged by size:
2531 1912 696 443 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2478 4309 4341 4383
Size: 32 135 32 874 32 1831 32 42
Cuts at: 4383 4415 1373
Size: 32 2626
Fragments arranged by size:
2626 1831 874 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 3012 3258 669
Size: 988 281 543 531 246 3079
Fragments arranged by size:
3079 988 543 531 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2917 4782 4966 1305
Size: 1612 1865 184 2007
Fragments arranged by size:
2007 1865 1612 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 10
Size: 54 1425 343 273 3573
Fragments arranged by size:
3573 1425 343 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3626 3985 4144 518
Size: 648 2460 359 159 2042
Fragments arranged by size:
2460 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5116
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NruI TCG'CGA
Cuts at: 2907 2907
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2878 1348
Size: 1530 4138
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 1108
Size: 1164 4504
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 2874 2767
Size: 107 5561
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PstI C_TGCA'G
Cuts at: 830 2498 3020 830
Size: 1668 522 3478
Fragments arranged by size:
3478 1668 522
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SacI G_AGCT'C
Cuts at: 2099 2630 2099
Size: 531 5137
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SexAI A'CCwGG_T
Cuts at: 2085 2870 2085
Size: 785 4883
SfcI C'TryA_G
Cuts at: 826 1045 2494 3016 4151 4832 5023 826
Size: 219 1449 522 1135 681 191 1471
Fragments arranged by size:
1471 1449 1135 681 522 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 3107 513
Size: 552 2042 3074
Fragments arranged by size:
3074 2042 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 2425 2605 3695 3712 3865 4050
Size: 1255 801 44 180 1090 17 153 185
Cuts at: 4050 4495 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1090 801 445 185 180 153
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 3914 5626
Size: 274 80 33 51 1443 342 1595 1712
Cuts at: 5626 96
Size: 138
Fragments arranged by size:
1712 1595 1443 342 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2448 2480 5628 5660 2448
Size: 32 3148 32 2456
Fragments arranged by size:
3148 2456 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2291 2543 2590 1316
Size: 975 252 47 4394
Fragments arranged by size:
4394 975 252 47
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2254 2875 2254
Size: 621 5047
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 2183 3797 2183
Size: 1614 4054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI