Restriction map for
pFN21AB7387
(Circular) (Six-base) MAPSORT of: pFN21AB7387.seq Check: 1753 from: 1 to: 5995
pFN21AB7387 5995 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2749 3085 4058 5442 5710 1632
Size: 1117 336 973 1384 268 1917
Fragments arranged by size:
1917 1384 1117 973 336 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 2379 3738 5310 2379
Size: 1359 1572 3064
Fragments arranged by size:
3064 1572 1359
ApaLI G'TGCA_C
Cuts at: 3195 4052 5208 3195
Size: 857 1156 3982
Fragments arranged by size:
3982 1156 857
ApoI r'AATT_y
Cuts at: 1493 2516 2562 2587 2883 3304 3530 3682 5791
Size: 1023 46 25 296 421 226 152 2109
Cuts at: 5791 1493
Size: 1697
Fragments arranged by size:
2109 1697 1023 421 296 226 152 46
25
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3228 3320 1870
Size: 73 1285 92 4545
Fragments arranged by size:
4545 1285 92 73
AvrII C'CTAG_G
Cuts at: 2392 2392
Size: 5995
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2165 2198 2165
Size: 33 5962
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4771 618
Size: 325 217 627 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3314 1391
Size: 1923 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3248 3282 4186 4220 1903
Size: 34 1311 34 904 34 3678
Fragments arranged by size:
3678 1311 904 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2254 2853 3887 5097 2254
Size: 599 1034 1210 3152
Fragments arranged by size:
3152 1210 1034 599
BclI T'GATC_A
Cuts at: 1548 2719 1548
Size: 1171 4824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2045 5990 2045
Size: 3945 2050
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 3199 4056 5212 1790
Size: 1409 857 1156 2573
Fragments arranged by size:
2573 1409 1156 857
BmgBI CAC'GTC
Cuts at: 1318 2143 1318
Size: 825 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2186 2739 3726
Size: 416 53 83 399 24 769 553 987
Cuts at: 3726 4684 442
Size: 958 1753
Fragments arranged by size:
1753 987 958 769 553 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2997 4655 1239
Size: 932 826 1658 2579
Fragments arranged by size:
2579 1658 932 826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 4120 4985 5283 2525
Size: 1595 865 298 3237
Fragments arranged by size:
3237 1595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4658 882
Size: 617 3159 2219
Fragments arranged by size:
3159 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2653 3786 493
Size: 711 1449 1133 2702
Fragments arranged by size:
2702 1449 1133 711
BsaBI GATnn'nnATC
Cuts at: 2265 2331 2418 5989 2265
Size: 66 87 3571 2271
Fragments arranged by size:
3571 2271 87 66
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3802 4184 4819 275
Size: 53 83 186 936 2269 382 635 1451
Fragments arranged by size:
2269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3059 4428 4826 5100 5247 5658 3059
Size: 1369 398 274 147 411 3396
Fragments arranged by size:
3396 1369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5770
Fragments arranged by size:
5770 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2111 1186
Size: 838 87 5070
Fragments arranged by size:
5070 838 87
BseYI C'CCAG_C
Cuts at: 5198 5694 5198
Size: 496 5499
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3199 4056 4141 5212 3199
Size: 857 85 1071 3982
Fragments arranged by size:
3982 1071 857 85
BsmI GAATG_Cn'
Cuts at: 2941 3506 3599 2941
Size: 565 93 5337
Fragments arranged by size:
5337 565 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2133 2133
Size: 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3199 3314 4056 4141 5212 1391
Size: 399 1409 115 742 85 1071 2174
Fragments arranged by size:
2174 1409 1071 742 399 115 85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3350 844
Size: 2506 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2920 4490 4664 65
Size: 1183 1672 1570 174 1396
Fragments arranged by size:
1672 1570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4639 4826 1700
Size: 110 2829 187 2869
Fragments arranged by size:
2869 2829 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 2045 3325 3692 4077 4094 5546 5632 5644
Size: 73 1280 367 385 17 1452 86 12
Cuts at: 5644 5990 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1280 385 367 346 86 73
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 513
Size: 552 939 75 4429
Fragments arranged by size:
4429 939 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2902 2937 529
Size: 35 2338 35 3587
Fragments arranged by size:
3587 2338 35 35
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 3442 4331
Size: 54 1425 50 275 18 246 1366 889
Cuts at: 4331 5960 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1366 889 275 246 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3925 1353
Size: 2572 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2749 2997 3085 4058 4655 5442
Size: 393 539 578 248 88 973 597 787
Cuts at: 5442 5710 1239
Size: 268 1524
Fragments arranged by size:
1524 973 787 597 578 539 393 268
248 88
EcoRI G'AATT_C
Cuts at: 2562 2587 2883 3304 3682 2562
Size: 25 296 421 378 4875
Fragments arranged by size:
4875 421 378 296 25
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 5142 1678
Size: 3464 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 2623 2655 2848
Size: 32 135 32 705 32 314 32 193
Cuts at: 2848 2880 3100 3132 4636 4668 4710 4742 1373
Size: 32 220 32 1504 32 42 32 2626
Fragments arranged by size:
2626 1504 705 314 220 193 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2304 3339 3585 669
Size: 988 281 366 1035 246 3079
Fragments arranged by size:
3079 1035 988 366 281 246
HindIII A'AGCT_T
Cuts at: 748 3012 748
Size: 2264 3731
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 5109 5293 1305
Size: 891 2913 184 2007
Fragments arranged by size:
2913 2007 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 3953 4312 4471 518
Size: 648 1105 1682 359 159 2042
Fragments arranged by size:
2042 1682 1105 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 3411 4088 5236 5481 1606
Size: 504 1301 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 677 504 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3353 4898 1132
Size: 97 2124 1545 2229
Fragments arranged by size:
2229 2124 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2737 3347 830
Size: 1907 610 3478
Fragments arranged by size:
3478 1907 610
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2110 2110
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2158 2733 3343 4478 5159 5350 826
Size: 219 1113 575 610 1135 681 191 1471
Fragments arranged by size:
1471 1135 1113 681 610 575 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 4099 5000 5262 820
Size: 197 926 561 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 561 262 197
SnaBI TAC'GTA
Cuts at: 493 2653 3786 493
Size: 2160 1133 2702
Fragments arranged by size:
2702 2160 1133
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 2320 3919 5
Size: 47 2268 1599 2081
Fragments arranged by size:
2268 2081 1599 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 4750
StyI C'CwwG_G
Cuts at: 513 1065 2004 2392 3434 513
Size: 552 939 388 1042 3074
Fragments arranged by size:
3074 1042 939 552 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2224 4022 4039 4192 4377 4822 325
Size: 1255 644 1798 17 153 185 445 1498
Fragments arranged by size:
1798 1498 1255 644 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4241 5953 96
Size: 274 80 33 51 1443 2264 1712 138
Fragments arranged by size:
2264 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 2566 2762 2930 4124 2566
Size: 196 168 1194 4437
Fragments arranged by size:
4437 1194 196 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI