Restriction map for
pFN21AB7383
(Circular) (Six-base) MAPSORT of: pFN21AB7383.seq Check: 9871 from: 1 to: 5755
pFN21AB7383 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2482 2878 2482
Size: 396 5359
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2287 3098 1937
Size: 350 811 4594
Fragments arranged by size:
4594 811 350
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3054 3882 4255 1563
Size: 1491 828 373 3063
Fragments arranged by size:
3063 1491 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2664 3818 5202 5470 1632
Size: 596 436 1154 1384 268 1917
Fragments arranged by size:
1917 1384 1154 596 436 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2162 3064 3290 3442 5551 1493
Size: 669 902 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 669 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 2999 3080 1870
Size: 73 987 69 81 4545
Fragments arranged by size:
4545 987 81 73 69
BamHI G'GATC_C
Cuts at: 2243 3085 3452 2243
Size: 842 367 4546
Fragments arranged by size:
4546 842 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2216 2322 2432 928
Size: 971 317 106 110 4251
Fragments arranged by size:
4251 971 317 110 106
BbvCI CC'TCA_GC
Cuts at: 2833 2845 2902 2959 2971 3030 2833
Size: 12 57 57 12 59 5558
Fragments arranged by size:
5558 59 57 57 12 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2695 4366 136
Size: 107 122 71 631 605 1023 1671 1525
Fragments arranged by size:
1671 1525 1023 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2033 3172 2033
Size: 1139 4616
Bme1580I G_kGCm'C
Cuts at: 1790 3816 4972 1790
Size: 2026 1156 2573
Fragments arranged by size:
2573 2026 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4415 1239
Size: 3176 2579
Bpu10I CC'TnA_GC
Cuts at: 2017 2786 2833 2845 2902 2959 2971 3030 2017
Size: 769 47 12 57 57 12 59 4742
Fragments arranged by size:
4742 769 59 57 57 47 12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2846 2903 2972 3031 3880 4745 5043 2777
Size: 69 57 69 59 849 865 298 3489
Fragments arranged by size:
3489 865 849 298 69 69 59 57
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 2856 2913 2982 3041 5749 2856
Size: 57 69 59 2708 2862
Fragments arranged by size:
2862 2708 69 59 57
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2739 1186
Size: 1553 4202
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2164 1163
Size: 573 428 4754
Fragments arranged by size:
4754 573 428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2475 3816 3901 4972 2475
Size: 1341 85 1071 3258
Fragments arranged by size:
3258 1341 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 2229 2229
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2475 3074 3816 3901 4972 1391
Size: 399 685 599 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 685 599 399 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 2401 3644 5374 2401
Size: 1243 1730 2782
Fragments arranged by size:
2782 1730 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2482 2878 3110 844
Size: 1638 396 232 3489
Fragments arranged by size:
3489 1638 396 232
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 3642 1394
Size: 429 214 1605 3507
Fragments arranged by size:
3507 1605 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 2254 96
Size: 2158 3597
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2575 794
Size: 1781 3974
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2243 3085 3452 3837 3854 5306 5392 5404
Size: 271 842 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 842 385 367 346 271 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2247 513
Size: 552 1182 4021
Fragments arranged by size:
4021 1182 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2667 3271 4065 4085 2667
Size: 604 794 20 4337
Fragments arranged by size:
4337 794 604 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 2721 3059 2721
Size: 338 5417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2664 3818 4415 5202 5470 1239
Size: 393 596 436 1154 597 787 268 1524
Fragments arranged by size:
1524 1154 787 597 596 436 393 268
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 5755
FspI TGC'GCA
Cuts at: 2568 4261 2568
Size: 1693 4062
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2381 2595 2627 4396
Size: 32 135 32 777 32 214 32 1769
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1769 777 214 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2395 3099 3345 669
Size: 988 281 457 704 246 3079
Fragments arranged by size:
3079 988 704 457 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2777 4869 5053 1305
Size: 996 476 2092 184 2007
Fragments arranged by size:
2092 2007 996 476 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2546 2552 3713 4072 4231 518
Size: 648 975 405 6 1161 359 159 2042
Fragments arranged by size:
2042 1161 975 648 405 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2711 2849 2906 2975 3034 3171 3848 4996
Size: 1105 138 57 69 59 137 677 1148
Cuts at: 4996 5241 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1105 677 245 138 137 69
59 57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PfoI T'CCnGG_A
Cuts at: 2310 2310
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2091 1613
Size: 478 5277
PsiI TTA'TAA
Cuts at: 2160 3325 2160
Size: 1165 4590
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2711 2711
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2599 3103 4238 4919 5110 826
Size: 219 1554 504 1135 681 191 1471
Fragments arranged by size:
1554 1471 1135 681 504 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2792 2861 2918 2987 3046 3859
Size: 197 926 849 69 57 69 59 813
Cuts at: 3859 4760 5022 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 849 813 262 197 69
69 59 57
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2123 3194 513
Size: 552 1058 1071 3074
Fragments arranged by size:
3074 1071 1058 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2254 4001 5713
Size: 274 80 33 51 1443 277 1747 1712
Cuts at: 5713 96
Size: 138
Fragments arranged by size:
1747 1712 1443 277 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2809 2841 2935 2967 3006 3038 5715 5747 2809
Size: 32 94 32 39 32 2677 32 2817
Fragments arranged by size:
2817 2677 94 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2096 2096
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 2556 3884 2556
Size: 1328 4427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI