Restriction map for
pFN21AB7381
(Circular) (Six-base) MAPSORT of: pFN21AB7381.seq Check: 2851 from: 1 to: 6091
pFN21AB7381 6091 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 2693 2677
Size: 16 6075
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2416 3434 1937
Size: 479 1018 4594
Fragments arranged by size:
4594 1018 479
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 2914 3044 4154 5538 5806 1632
Size: 944 338 130 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 944 338 268 130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2232 4990 5967 1203
Size: 1029 2758 977 1327
Fragments arranged by size:
2758 1327 1029 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 2344 3834 5406 2344
Size: 1490 1572 3029
Fragments arranged by size:
3029 1572 1490
ApaI G_GGCC'C
Cuts at: 2797 2946 3113 3262 3371 2797
Size: 149 167 149 109 5517
Fragments arranged by size:
5517 167 149 149 109
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2763 3223 3400 3626 3778 5887 1493
Size: 1270 460 177 226 152 2109 1697
Fragments arranged by size:
2109 1697 1270 460 226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 2314 2936 3254 3416 1870
Size: 73 182 189 622 318 162 4545
Fragments arranged by size:
4545 622 318 189 182 162 73
BamHI G'GATC_C
Cuts at: 2974 3421 3788 2974
Size: 447 367 5277
Fragments arranged by size:
5277 447 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2365 2397 2699 3412 4867
Size: 325 217 627 578 32 302 713 1455
Cuts at: 4867 618
Size: 1842
Fragments arranged by size:
1842 1455 713 627 578 325 302 217
32
BanII G_rGCy'C
Cuts at: 1391 2126 2484 2657 2797 2875 2946 3032 3113
Size: 735 358 173 140 78 71 86 81
Cuts at: 3113 3262 3371 3410 1391
Size: 149 109 39 4072
Fragments arranged by size:
4072 735 358 173 149 140 109 86
81 78 71 39
BbeI G_GCGC'C
Cuts at: 2401 2401
Size: 6091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2825 928
Size: 971 926 4194
Fragments arranged by size:
4194 971 926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2200 2234 2305 2339 4282
Size: 34 50 34 179 34 71 34 1943
Cuts at: 4282 4316 1903
Size: 34 3678
Fragments arranged by size:
3678 1943 179 71 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2499 6086 2499
Size: 3587 2504
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2370 2704 2797 2946 3113 3262 3371 4152
Size: 580 334 93 149 167 149 109 781
Cuts at: 4152 5308 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 781 580 334 167 149 149
109 93
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 5110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 2432 3108
Size: 416 53 83 399 24 762 253 676
Cuts at: 3108 3822 4780 442
Size: 714 958 1753
Fragments arranged by size:
1753 958 762 714 676 416 399 253
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 2366 2841 4751 1239
Size: 901 226 475 1910 2579
Fragments arranged by size:
2579 1910 901 475 226
Bpu10I CC'TnA_GC
Cuts at: 2279 2483 2773 3201 2279
Size: 204 290 428 5169
Fragments arranged by size:
5169 428 290 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3054 4216 5081 5379 3054
Size: 1162 865 298 3766
Fragments arranged by size:
3766 1162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2398 3898 4280 4915
Size: 53 83 186 936 865 1500 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1500 1451 936 865 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 1178
Size: 30 165 30 1332 30 4504
Fragments arranged by size:
4504 1332 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6091
BseYI C'CCAG_C
Cuts at: 2045 3106 3298 5294 5790 2045
Size: 1061 192 1996 496 2346
Fragments arranged by size:
2346 1996 1061 496 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 2471 2921 1163
Size: 573 717 18 450 4333
Fragments arranged by size:
4333 717 573 450 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2332 2392 3541 4302 5330 1540
Size: 275 186 331 60 1149 761 1028 2301
Fragments arranged by size:
2301 1149 1028 761 331 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2462 2484 2657 4152 4237 5308 2462
Size: 22 173 1495 85 1071 3245
Fragments arranged by size:
3245 1495 1071 173 85 22
BsmI GAATG_Cn'
Cuts at: 2956 3602 3695 2956
Size: 646 93 5352
Fragments arranged by size:
5352 646 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2370 2462 2484 2657 2704 2797
Size: 399 336 244 92 22 173 47 93
Cuts at: 2797 2875 2946 3032 3113 3262 3371 3410 4152
Size: 78 71 86 81 149 109 39 742
Cuts at: 4152 4237 5308 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 336 244 173 149
109 93 92 86 85 81 78 71
47 39 22
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2677 2693 3094 3446 844
Size: 1833 16 401 352 3489
Fragments arranged by size:
3489 1833 401 352 16
BsrBI CCG'CTC
Cuts at: 1394 1823 2130 3190 3978 1394
Size: 429 307 1060 788 3507
Fragments arranged by size:
3507 1060 788 429 307
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 4586 4760 65
Size: 1183 1366 1972 174 1396
Fragments arranged by size:
1972 1396 1366 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 794
Size: 1646 4445
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2499 2974 3421 3788 4173 4190 5642 5728
Size: 527 475 447 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 527 475 447 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2834 2834
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2031 2536 513
Size: 552 966 505 4068
Fragments arranged by size:
4068 966 552 505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2882 3607 4401 4421 2882
Size: 725 794 20 4552
Fragments arranged by size:
4552 794 725 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 2389 3538
Size: 54 1425 50 275 18 499 60 1149
Cuts at: 3538 4427 6056 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1149 889 499 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2329 2389 3538 1537
Size: 275 517 60 1149 4090
Fragments arranged by size:
4090 1149 517 275 60
EarI CTCTTCn'nnn_
Cuts at: 1353 4021 1353
Size: 2668 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2655 2482
Size: 173 5918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2366 2576 2841 2914 3044 4154
Size: 393 508 226 210 265 73 130 1110
Cuts at: 4154 4751 5538 5806 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1110 787 597 508 393 268 265
226 210 130 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2967 2708
Size: 259 5832
EcoO109I rG'GnC_Cy
Cuts at: 2042 2092 2407 2713 2793 2794 2942 2999 3092
Size: 50 315 306 80 1 148 57 93
Cuts at: 3092 3110 3244 3258 3259 2042
Size: 18 134 14 1 4874
Fragments arranged by size:
4874 315 306 148 134 93 80 57
50 18 14 1 1
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2018 2084 2401 5238 1678
Size: 340 66 317 2837 2531
Fragments arranged by size:
2837 2531 340 317 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2524 2556 2560 2592 2812
Size: 32 135 32 952 32 4 32 220
Cuts at: 2812 2844 4732 4764 4806 4838 1373
Size: 32 1888 32 42 32 2626
Fragments arranged by size:
2626 1888 952 220 135 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2417 3435 3681 669
Size: 988 281 479 1018 246 3079
Fragments arranged by size:
3079 1018 988 479 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 2397 2397
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4269
Fragments arranged by size:
4269 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4049 4408 4567 518
Size: 648 2883 359 159 2042
Fragments arranged by size:
2883 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2118 2394 3507 4184 5332 5577 1606
Size: 415 97 276 1113 677 1148 245 2120
Fragments arranged by size:
2120 1148 1113 677 415 276 245 97
NarI GG'CG_CC
Cuts at: 2398 2398
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 2329 3538 2329
Size: 1209 4882
NspI r_CATG'y
Cuts at: 1132 1229 2236 3449 4994 1132
Size: 97 1007 1213 1545 2229
Fragments arranged by size:
2229 1545 1213 1007 97
PasI CC'CwG_GG
Cuts at: 1348 2979 3236 3304 1348
Size: 1631 257 68 4135
Fragments arranged by size:
4135 1631 257 68
PciI A'CATG_T
Cuts at: 2232 4990 2232
Size: 2758 3333
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 2910 2910
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2407 2713 3244 2092
Size: 315 306 531 4939
Fragments arranged by size:
4939 531 315 306
PshAI GACnn'nnGTC
Cuts at: 1613 2412 1613
Size: 799 5292
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2793 2942 3109 3258 3367 2793
Size: 149 167 149 109 5517
Fragments arranged by size:
5517 167 149 149 109
PstI C_TGCA'G
Cuts at: 830 2712 3091 3443 830
Size: 1882 379 352 3478
Fragments arranged by size:
3478 1882 379 352
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2021 2021
Size: 6091
SacI G_AGCT'C
Cuts at: 2484 2657 2484
Size: 173 5918
SalI G'TCGA_C
Cuts at: 1936 2415 3433 1936
Size: 479 1018 4594
Fragments arranged by size:
4594 1018 479
SanDI GG'GwC_CC
Cuts at: 2092 2092
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3091 3443 3091
Size: 352 5739
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SexAI A'CCwGG_T
Cuts at: 2695 2695
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 2708 3087 3439 4574 5255 5446 826
Size: 219 1663 379 352 1135 681 191 1471
Fragments arranged by size:
1663 1471 1135 681 379 352 219 191
SfoI GGC'GCC
Cuts at: 2399 2399
Size: 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3069 4195 5096 5358 820
Size: 197 926 1126 1126 901 262 1553
Fragments arranged by size:
1553 1126 1126 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2169 3121 3177 3288 3530 513
Size: 552 1104 952 56 111 242 3074
Fragments arranged by size:
3074 1104 952 552 242 111 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4118 4135 4288 4473 4918 325
Size: 1255 2538 17 153 185 445 1498
Fragments arranged by size:
2538 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 6049 96
Size: 274 80 33 51 1443 2360 1712 138
Fragments arranged by size:
2360 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 2297 1316
Size: 981 5110
XbaI T'CTAG_A
Cuts at: 3097 3427 3097
Size: 330 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SapI SfiI SrfI SwaI