Restriction map for
pFN21AB7378
(Circular) (Six-base) MAPSORT of: pFN21AB7378.seq Check: 4406 from: 1 to: 5539
pFN21AB7378 5539 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2439 2506 2439
Size: 67 5472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3602 4986 5254 1632
Size: 1970 1384 268 1917
Fragments arranged by size:
1970 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 2379 3282 4854 2379
Size: 903 1572 3064
Fragments arranged by size:
3064 1572 903
ApaI G_GGCC'C
Cuts at: 2660 2660
Size: 5539
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2168 2303 2864 1870
Size: 73 131 94 135 561 4545
Fragments arranged by size:
4545 561 135 131 94 73
BamHI G'GATC_C
Cuts at: 2085 2869 3236 2085
Size: 784 367 4388
Fragments arranged by size:
4388 784 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2146 2164 2309 2339
Size: 325 217 627 319 40 18 145 30
Cuts at: 2339 2594 2860 4315 618
Size: 255 266 1455 1842
Fragments arranged by size:
1842 1455 627 325 319 266 255 217
145 40 30 18
BanII G_rGCy'C
Cuts at: 1391 2319 2660 2858 1391
Size: 928 341 198 4072
Fragments arranged by size:
4072 928 341 198
BbeI G_GCGC'C
Cuts at: 2110 2150 2168 2110
Size: 40 18 5481
Fragments arranged by size:
5481 40 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3730 3764 1903
Size: 34 50 34 1709 34 3678
Fragments arranged by size:
3678 1709 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 2691 1548
Size: 1143 4396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2247 2294 4150
Size: 107 122 71 631 605 575 47 1856
Cuts at: 4150 136
Size: 1525
Fragments arranged by size:
1856 1525 631 605 575 122 107 71
47
BglII A'GATC_T
Cuts at: 2788 5534 2788
Size: 2746 2793
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2312 2597 2660 3600 4756 1790
Size: 522 285 63 940 1156 2573
Fragments arranged by size:
2573 1156 940 522 285 63
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 4762
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2549 3270 4228
Size: 416 53 83 399 24 1132 721 958
Cuts at: 4228 442
Size: 1753
Fragments arranged by size:
1753 1132 958 721 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 4199 1239
Size: 1388 1572 2579
Fragments arranged by size:
2579 1572 1388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2565 3330 493
Size: 711 1361 765 2702
Fragments arranged by size:
2702 1361 765 711
BsaBI GATnn'nnATC
Cuts at: 2690 5533 2690
Size: 2843 2696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2107 2147 2165 3346
Size: 53 83 186 936 574 40 18 1181
Cuts at: 3346 3728 4363 275
Size: 382 635 1451
Fragments arranged by size:
1451 1181 936 635 574 382 186 83
53 40 18
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 1186
Size: 1438 4101
BseYI C'CCAG_C
Cuts at: 2291 2372 2818 4742 5238 2291
Size: 81 446 1924 496 2592
Fragments arranged by size:
2592 1924 496 446 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 1163
Size: 573 681 4285
Fragments arranged by size:
4285 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2104 2362 2989 3750 4778 1540
Size: 275 186 103 258 627 761 1028 2301
Fragments arranged by size:
2301 1028 761 627 275 258 186 103
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2319 2597 2660 2858 3600 3685
Size: 399 522 7 278 63 198 742 85
Cuts at: 3685 4756 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 522 399 278 198 85
63 7
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2439 2506 2894 844
Size: 1595 67 388 3489
Fragments arranged by size:
3489 1595 388 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2127 2246 4183 4370 1700
Size: 110 293 24 119 1937 187 2869
Fragments arranged by size:
2869 1937 293 187 119 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 2328 2382 2395 2415 2441 1925
Size: 403 54 13 20 26 5023
Fragments arranged by size:
5023 403 54 26 20 13
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2346 794
Size: 1552 3987
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 2426 1184
Size: 1242 4297
BstXI CCAn_nnnn'nTGG
Cuts at: 2291 2749 2291
Size: 458 5081
BstYI r'GATC_y
Cuts at: 1972 2085 2172 2788 2869 3236 3621 3638 5090
Size: 113 87 616 81 367 385 17 1452
Cuts at: 5090 5176 5188 5534 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 616 385 367 346 113 87
86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2589 2589
Size: 5539
BtgI C'CryG_G
Cuts at: 513 1065 2676 513
Size: 552 1611 3376
Fragments arranged by size:
3376 1611 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 2129 2986
Size: 54 1425 50 275 18 271 28 857
Cuts at: 2986 3875 5504 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 857 275 271 54 50
43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2101 2986 1537
Size: 275 289 885 4090
Fragments arranged by size:
4090 885 289 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 3602 4199 4986 5254 1239
Size: 393 995 975 597 787 268 1524
Fragments arranged by size:
1524 995 975 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2705 2435
Size: 270 5269
EcoO109I rG'GnC_Cy
Cuts at: 2656 2657 2656
Size: 1 5538
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2110 2150 2168 2183 2257 2303 4686 1678
Size: 432 40 18 15 74 46 2383 2531
Fragments arranged by size:
2531 2383 432 74 46 40 18 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 2747 2779 4180
Size: 32 135 32 776 32 367 32 1401
Cuts at: 4180 4212 4254 4286 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1401 776 367 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KasI G'GCGC_C
Cuts at: 2106 2146 2164 2106
Size: 40 18 5481
Fragments arranged by size:
5481 40 18
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 4653 4837 1305
Size: 1074 2274 184 2007
Fragments arranged by size:
2274 2007 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 3856 4015 518
Size: 648 2331 359 159 2042
Fragments arranged by size:
2331 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2056 2068 2208 2291 2955 3632 4780
Size: 426 24 12 140 83 664 677 1148
Cuts at: 4780 5025 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 664 426 245 140 83
24 12
NaeI GCC'GGC
Cuts at: 2105 2129 2248 2105
Size: 24 119 5396
Fragments arranged by size:
5396 119 24
NarI GG'CG_CC
Cuts at: 2107 2147 2165 2107
Size: 40 18 5481
Fragments arranged by size:
5481 40 18
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4987
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NgoMIV G'CCGG_C
Cuts at: 2103 2127 2246 2103
Size: 24 119 5396
Fragments arranged by size:
5396 119 24
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 2682 1108
Size: 1497 77 3965
Fragments arranged by size:
3965 1497 77
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PmlI CAC'GTG
Cuts at: 2565 2565
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PspOMI G'GGCC_C
Cuts at: 2656 2656
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2891 830
Size: 2061 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2887 4022 4703 4894 826
Size: 219 1842 1135 681 191 1471
Fragments arranged by size:
1842 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2108 2148 2166 2108
Size: 40 18 5481
Fragments arranged by size:
5481 40 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 2246 1700
Size: 546 4993
SmaI CCC'GGG
Cuts at: 1872 2170 2866 1872
Size: 298 696 4545
Fragments arranged by size:
4545 696 298
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2080 2978 513
Size: 552 1015 898 3074
Fragments arranged by size:
3074 1015 898 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 3566 3583 3736 3921 4366 325
Size: 1255 1032 954 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1032 954 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 2627 1316
Size: 1311 4228
XbaI T'CTAG_A
Cuts at: 2785 2875 2785
Size: 90 5449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2562 2562
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2168 2864 1870
Size: 298 696 4545
Fragments arranged by size:
4545 696 298
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI