Restriction map for
pFN21AB7377
(Circular) (Six-base) MAPSORT of: pFN21AB7377.seq Check: 3239 from: 1 to: 6052
pFN21AB7377 6052 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2258 2695 3030 2258
Size: 437 335 5280
Fragments arranged by size:
5280 437 335
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 3395 1937
Size: 847 611 4594
Fragments arranged by size:
4594 847 611
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4115 5499 5767 1632
Size: 2483 1384 268 1917
Fragments arranged by size:
2483 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 4781 4781
Size: 6052
AleI CACnn'nnGTG
Cuts at: 1166 2655 2681 1166
Size: 1489 26 4537
Fragments arranged by size:
4537 1489 26
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6052
AlwNI CAG_nnn'CTG
Cuts at: 3058 3795 5367 3058
Size: 737 1572 3743
Fragments arranged by size:
3743 1572 737
ApaI G_GGCC'C
Cuts at: 2057 2057
Size: 6052
ApaLI G'TGCA_C
Cuts at: 2596 4109 5265 2596
Size: 1513 1156 3383
Fragments arranged by size:
3383 1513 1156
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2875 3280 3377 1870
Size: 73 932 405 97 4545
Fragments arranged by size:
4545 932 405 97 73
BamHI G'GATC_C
Cuts at: 2198 3382 3749 2198
Size: 1184 367 4501
Fragments arranged by size:
4501 1184 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 2646 2989 3373 4828
Size: 325 217 627 542 317 343 384 1455
Cuts at: 4828 618
Size: 1842
Fragments arranged by size:
1842 1455 627 542 384 343 325 317
217
BanII G_rGCy'C
Cuts at: 1391 2057 2064 2925 3371 1391
Size: 666 7 861 446 4072
Fragments arranged by size:
4072 861 666 446 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2704 3230 928
Size: 971 805 526 3750
Fragments arranged by size:
3750 971 805 526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4243 4277 1903
Size: 34 50 34 2222 34 3678
Fragments arranged by size:
3678 2222 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2496 3944 5154 2496
Size: 1448 1210 3394
Fragments arranged by size:
3394 1448 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4663 136
Size: 107 122 71 631 605 337 2654 1525
Fragments arranged by size:
2654 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2271 6047 2271
Size: 3776 2276
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2334 2600 2649 4113 5269 1790
Size: 267 277 266 49 1464 1156 2573
Fragments arranged by size:
2573 1464 1156 277 267 266 49
BmgBI CAC'GTC
Cuts at: 1318 2551 1318
Size: 1233 4819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2530 3783 4741
Size: 416 53 83 399 24 1113 1253 958
Cuts at: 4741 442
Size: 1753
Fragments arranged by size:
1753 1253 1113 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2113 2288 2794 2936 3227 4712 1239
Size: 809 65 175 506 142 291 1485 2579
Fragments arranged by size:
2579 1485 809 506 291 175 142 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3025 4177 5042 5340 3025
Size: 1152 865 298 3737
Fragments arranged by size:
3737 1152 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4715 882
Size: 617 3216 2219
Fragments arranged by size:
3216 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2611 3843 493
Size: 711 1407 1232 2702
Fragments arranged by size:
2702 1407 1232 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 3859 4241 4876
Size: 53 83 186 936 912 1414 382 635
Cuts at: 4876 275
Size: 1451
Fragments arranged by size:
1451 1414 936 912 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4485 4883 5157 5304 5715 4485
Size: 398 274 147 411 4822
Fragments arranged by size:
4822 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2813 2843 1178
Size: 30 165 30 1410 30 4387
Fragments arranged by size:
4387 1410 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2341 2933 2999 1186
Size: 1155 592 66 4239
Fragments arranged by size:
4239 1155 592 66
BseYI C'CCAG_C
Cuts at: 2050 2308 2825 2998 3127 3344 5255 5751 2050
Size: 258 517 173 129 217 1911 496 2351
Fragments arranged by size:
2351 1911 517 496 258 217 173 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2191 3053 1163
Size: 573 455 862 4162
Fragments arranged by size:
4162 862 573 455
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2600 4113 4198 5269 2106
Size: 494 1513 85 1071 2889
Fragments arranged by size:
2889 1513 1071 494 85
BsmI GAATG_Cn'
Cuts at: 2777 3563 3656 2777
Size: 786 93 5173
Fragments arranged by size:
5173 786 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2064 2106 2334 2600 2649 2925
Size: 399 267 7 42 228 266 49 276
Cuts at: 2925 3371 4113 4198 5269 1391
Size: 446 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 276 267 266
228 85 49 42 7
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2258 2313 2695 3030 3407 844
Size: 1414 55 382 335 377 3489
Fragments arranged by size:
3489 1414 382 377 335 55
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 4547 4721 65
Size: 1183 1345 1954 174 1396
Fragments arranged by size:
1954 1396 1345 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4696 4883 1700
Size: 110 2886 187 2869
Fragments arranged by size:
2886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 2681 4112 5124 2681
Size: 1431 1012 3609
Fragments arranged by size:
3609 1431 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2514 794
Size: 1720 4332
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6052
BstXI CCAn_nnnn'nTGG
Cuts at: 2657 2657
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2198 2271 3382 3749 4134 4151 5603 5689
Size: 226 73 1111 367 385 17 1452 86
Cuts at: 5689 5701 6047 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1111 385 367 346 226 86
73 17 12
BstZ17I GTA'TAC
Cuts at: 2785 2785
Size: 6052
BtgI C'CryG_G
Cuts at: 513 1065 2076 2404 2641 2740 513
Size: 552 1011 328 237 99 3825
Fragments arranged by size:
3825 1011 552 328 237 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 2045 2046 3568 4362 4382 2045
Size: 1 1522 794 20 3715
Fragments arranged by size:
3715 1522 794 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DraIII CAC_nnn'GTG
Cuts at: 2169 2524 2169
Size: 355 5697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2458 5059 809
Size: 507 1142 2601 1802
Fragments arranged by size:
2601 1802 1142 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2397 2790
Size: 54 1425 50 275 18 360 207 393
Cuts at: 2790 3499 4388 6017 8
Size: 709 889 1629 43
Fragments arranged by size:
1629 1425 889 709 393 360 275 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 3982 1353
Size: 1233 1396 3423
Fragments arranged by size:
3423 1396 1233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2855 4639 5013 5159 387
Size: 1064 138 1266 1784 374 146 1280
Fragments arranged by size:
1784 1280 1266 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2113 2288 2794 2936 3227 4115
Size: 393 416 65 175 506 142 291 888
Cuts at: 4115 4712 5499 5767 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 506 416 393 291
268 175 142 65
EcoO109I rG'GnC_Cy
Cuts at: 2283 2467 2627 2670 3284 2283
Size: 184 160 43 614 5051
Fragments arranged by size:
5051 614 184 160 43
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2914 2740
Size: 174 5878
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 5199 1678
Size: 3521 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4693 4725 4767 4799 1373
Size: 32 135 32 3121 32 42 32 2626
Fragments arranged by size:
3121 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3396 3642 669
Size: 988 281 1458 246 3079
Fragments arranged by size:
3079 1458 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2745 5166 5350 1305
Size: 1440 2421 184 2007
Fragments arranged by size:
2421 2007 1440 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2792 10
Size: 54 1425 343 567 393 3270
Fragments arranged by size:
3270 1425 567 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2655 2681 2792 4010 4369 4528 518
Size: 648 1489 26 111 1218 359 159 2042
Fragments arranged by size:
2042 1489 1218 648 359 159 111 26
MspA1I CmG'CkG
Cuts at: 1606 2175 2537 2850 3127 3468 4145 5293 5538
Size: 569 362 313 277 341 677 1148 245
Cuts at: 5538 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 569 362 341 313 277
245
NcoI C'CATG_G
Cuts at: 513 1065 2076 2404 513
Size: 552 1011 328 4161
Fragments arranged by size:
4161 1011 552 328
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2363 2396 3410 4955 1132
Size: 97 1134 33 1014 1545 2229
Fragments arranged by size:
2229 1545 1134 1014 97 33
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 5343
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 1108
Size: 969 5083
PfoI T'CCnGG_A
Cuts at: 2901 2901
Size: 6052
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 3284 2467
Size: 817 5235
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PspOMI G'GGCC_C
Cuts at: 2053 2053
Size: 6052
PstI C_TGCA'G
Cuts at: 830 2255 2706 3404 830
Size: 1425 451 698 3478
Fragments arranged by size:
3478 1425 698 451
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2175 3127 2175
Size: 952 5100
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SanDI GG'GwC_CC
Cuts at: 3284 3284
Size: 6052
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SexAI A'CCwGG_T
Cuts at: 2883 2883
Size: 6052
SfcI C'TryA_G
Cuts at: 826 1045 2251 2702 3400 4535 5216 5407 826
Size: 219 1206 451 698 1135 681 191 1471
Fragments arranged by size:
1471 1206 1135 698 681 451 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 2877 3379 1872
Size: 1005 502 4545
Fragments arranged by size:
4545 1005 502
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3040 4156 5057 5319 820
Size: 197 926 1097 1116 901 262 1553
Fragments arranged by size:
1553 1116 1097 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 2753 1517
Size: 1236 4816
StyI C'CwwG_G
Cuts at: 513 1065 2076 2404 3491 513
Size: 552 1011 328 1087 3074
Fragments arranged by size:
3074 1087 1011 552 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2512 4079 4096 4249 4434 4879 325
Size: 1255 932 1567 17 153 185 445 1498
Fragments arranged by size:
1567 1498 1255 932 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4298 6010 96
Size: 274 80 33 51 1443 2321 1712 138
Fragments arranged by size:
2321 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 2995 3027 6012 6044 2190
Size: 32 773 32 2985 32 2198
Fragments arranged by size:
2985 2198 773 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6052
XmaI C'CCGG_G
Cuts at: 1870 2875 3377 1870
Size: 1005 502 4545
Fragments arranged by size:
4545 1005 502
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI XcmI