Restriction map for
pFN21AB7376
(Circular) (Six-base) MAPSORT of: pFN21AB7376.seq Check: 2693 from: 1 to: 5695
pFN21AB7376 5695 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2103 2348 3758 5142 5410 1632
Size: 471 245 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1384 471 268 245
AfeI AGC'GCT
Cuts at: 2205 2205
Size: 5695
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2272 3438 5010 2272
Size: 1166 1572 2957
Fragments arranged by size:
2957 1572 1166
ApaLI G'TGCA_C
Cuts at: 2965 3752 4908 2965
Size: 787 1156 3752
Fragments arranged by size:
3752 1156 787
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2729 3020 1870
Size: 73 786 291 4545
Fragments arranged by size:
4545 786 291 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 4471 618
Size: 325 217 627 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3014 1391
Size: 1623 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 928
Size: 971 332 4392
Fragments arranged by size:
4392 971 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 2952 1548
Size: 1404 4291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 4306 136
Size: 107 122 71 631 605 526 2108 1525
Fragments arranged by size:
2108 1525 631 605 526 122 107 71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2969 3756 4912 1790
Size: 1179 787 1156 2573
Fragments arranged by size:
2573 1179 1156 787
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2791 4355 1239
Size: 1085 467 1564 2579
Fragments arranged by size:
2579 1564 1085 467
Bpu10I CC'TnA_GC
Cuts at: 2894 2894
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2895 3820 4685 4983 2165
Size: 730 925 865 298 2877
Fragments arranged by size:
2877 925 865 730 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2725 2966 4358 882
Size: 617 1226 241 1392 2219
Fragments arranged by size:
2219 1392 1226 617 241
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2588 2747 2861 1186
Size: 1207 195 159 114 4020
Fragments arranged by size:
4020 1207 195 159 114
BseYI C'CCAG_C
Cuts at: 2118 4898 5394 2118
Size: 2780 496 2419
Fragments arranged by size:
2780 2419 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2969 3756 3841 4912 2969
Size: 787 85 1071 3752
Fragments arranged by size:
3752 1071 787 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
BsmBI CGTCTCn'nnnn_
Cuts at: 2158 2539 2158
Size: 381 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2969 3014 3756 3841 4912 1391
Size: 399 1179 45 742 85 1071 2174
Fragments arranged by size:
2174 1179 1071 742 399 85 45
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2257 3050 844
Size: 1413 793 3489
Fragments arranged by size:
3489 1413 793
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2653 4339 4526 1700
Size: 110 843 1686 187 2869
Fragments arranged by size:
2869 1686 843 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2028 2736 3025 3392 3777 3794 5246 5332
Size: 56 708 289 367 385 17 1452 86
Cuts at: 5332 5344 5690 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 708 385 367 346 289 86
56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2219 2219
Size: 5695
BtgI C'CryG_G
Cuts at: 513 1065 2004 2538 2617 513
Size: 552 939 534 79 3591
Fragments arranged by size:
3591 939 552 534 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2969 3211 4005 4025 2969
Size: 242 794 20 4639
Fragments arranged by size:
4639 794 242 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2887 2999 2887
Size: 112 5583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 3142 4031
Size: 54 1425 50 275 18 711 601 889
Cuts at: 4031 5660 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 711 601 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2940 3625 1353
Size: 860 727 685 3423
Fragments arranged by size:
3423 860 727 685
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2651 4282 4656 4802 387
Size: 1064 138 1062 1631 374 146 1280
Fragments arranged by size:
1631 1280 1064 1062 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2324 2348 2791 3758 4355 5142
Size: 393 471 221 24 443 967 597 787
Cuts at: 5142 5410 1239
Size: 268 1524
Fragments arranged by size:
1524 967 787 597 471 443 393 268
221 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 2594 2636 2744 2570
Size: 24 42 108 5521
Fragments arranged by size:
5521 108 42 24
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2207 2525 4842 1678
Size: 529 318 2317 2531
Fragments arranged by size:
2531 2317 529 318
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373
Size: 32 135 32 2764 32 42 32 2626
Fragments arranged by size:
2764 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2767 748
Size: 2019 3676
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3653 4012 4171 518
Size: 648 2487 359 159 2042
Fragments arranged by size:
2487 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2134 2496 2540 2619 2685 2709 2826
Size: 516 12 362 44 79 66 24 117
Cuts at: 2826 3111 3788 4936 5181 1606
Size: 285 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 516 362 285 245 117
79 66 44 24 12
NaeI GCC'GGC
Cuts at: 2655 2655
Size: 5695
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 2653 2653
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2366 2407 3053 4598 1132
Size: 97 1137 41 646 1545 2229
Fragments arranged by size:
2229 1545 1137 646 97 41
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PfoI T'CCnGG_A
Cuts at: 2772 2772
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2170 1613
Size: 557 5138
PsiI TTA'TAA
Cuts at: 2069 3265 2069
Size: 1196 4499
PstI C_TGCA'G
Cuts at: 830 2139 3047 830
Size: 1309 908 3478
Fragments arranged by size:
3478 1309 908
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2122 2134 2709 2826 2122
Size: 12 575 117 4991
Fragments arranged by size:
4991 575 117 12
SacII CC_GC'GG
Cuts at: 2541 2620 2541
Size: 79 5616
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SapI GCTCTTCn'nnn_
Cuts at: 2213 2213
Size: 5695
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2135 3043 4178 4859 5050 826
Size: 219 1090 908 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 908 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2910 3799 4700 4962 820
Size: 197 926 201 766 889 901 262 1553
Fragments arranged by size:
1553 926 901 889 766 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 2366 3053 1132
Size: 1234 687 3774
Fragments arranged by size:
3774 1234 687
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 2642 2892 1517
Size: 1083 42 250 4320
Fragments arranged by size:
4320 1083 250 42
StyI C'CwwG_G
Cuts at: 513 1065 2004 2447 3134 513
Size: 552 939 443 687 3074
Fragments arranged by size:
3074 939 687 552 443
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 2795 3941
Size: 274 80 33 51 1443 255 563 1146
Cuts at: 3941 5653 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1146 563 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2713 2713
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI